Characteristics of an antioxidant activity of Yersinia pestis with different plasmid spectrum

• Main Authors: O. V. Yuryeva, V. I. Dubrovina, K. M. Korytov, G. B. Mukhturgin, T. A. Ivanova, S. A. Vityazeva, E. G. Tokmakova, S. V. Balakhonov
• Format: Article
• Language: Russian
• Published: Scientific Сentre for Family Health and Human Reproduction Problems 2016-09-01
• Series: Acta Biomedica Scientifica
• Subjects: yersinia pestis; plasmids; superoxide dismutase; peroxide destroying activity
• Online Access: https://www.actabiomedica.ru/jour/article/view/295
• ISSN: 2541-9420; 2587-9596

Experimental data concerning complex study of antioxidant activity of Y. pestis with different plasmid spectrum (wild-type Yersinia pestis subsp. pestis, Yersinia pestis subsp. altaica and their isogenic variants) are represented in the article. Superoxide dismutase activity of the tested Y. pestis strains was from 6.0 to 9.0 x 109 microbe cells. Significant differences between the Y. pestis strains with different plasmid composition were not detected by this parameter. Our results, consistent with the data of other authors, tell that superoxide dismutase activity is a thermo-inducible feature and does not depend on a plasmid spectrum. High peroxide destroying activity was also detected in all tested Y. pestis strains. The differences between the strains regarding their common peroxide destroying activity were found. This parameter of the plague microbe strains lacking pYP plasmid was at least 3 times lower than common peroxide destroying activity in strains with this plasmid in the genome. In our opinion these revealed differences were caused by characteristics of plasmid spectrum. The common peroxide destroying activity's degree of Y. pestis strains can be associated with the presence of pYP plasmid in the genome. The isogenic variants of Y. pestis strains lacking one of the plasmids had smaller pathogenic activity. This fact points to the need for further study of these strains.