Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells
Trypanosoma cruzi, the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi, which were previo...
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doaj-771b5aec3ae940a48d7a5dc8087f4e5b2020-11-25T02:41:57ZengTaylor & Francis GroupJournal of Extracellular Vesicles2001-30782018-12-017110.1080/20013078.2018.14637791463779Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cellsKleber Silva Ribeiro0Camilla Ioshida Vasconcellos1Rodrigo Pedro Soares2Maria Tays Mendes3Cameron C. Ellis4Marcela Aguilera-Flores5Igor Correia de Almeida6Sergio Schenkman7Leo Kei Iwai8Ana Claudia Torrecilhas9UNIFESPUNIFESPInstituto René Rachou/FIOCRUZ – MGUniversity of Texas at El Paso (UTEP)University of Texas at El Paso (UTEP)University of Texas at El Paso (UTEP)University of Texas at El Paso (UTEP)UNIFESPInstituto ButantanUNIFESPTrypanosoma cruzi, the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi, which were previously shown to differentially modulate host innate and acquired immune responses. Tissue culture-derived trypomastigotes of both strains secreted extracellular vesicles (EVs), as demonstrated by electron scanning microscopy. EVs were purified by exclusion chromatography or ultracentrifugation and quantitated using nanoparticle tracking analysis. Trypomastigotes from YuYu strain released higher number of EVs than those from Y strain, enriched with virulence factors trans-sialidase (TS) and cruzipain. Proteomic analysis confirmed the increased abundance of proteins coded by the TS gene family, mucin-like glycoproteins, and some typical exosomal proteins in the YuYu strain, which also showed considerable differences between purified EVs and vesicle-free fraction as compared to the Y strain. To evaluate whether such differences were related to parasite infectivity, J774 macrophages and LLC-MK2 kidney cells were preincubated with purified EVs from both strains and then infected with Y strain trypomastigotes. EVs released by YuYu strain caused a lower infection but higher intracellular proliferation in J774 macrophages than EVs from Y strain. In contrast, YuYu strain-derived EVs caused higher infection of LLC-MK2 cells than Y strain-derived EVs. In conclusion, quantitative and qualitative differences in EVs and secreted proteins from different T. cruzi strains may correlate with infectivity/virulence during the host–parasite interaction.http://dx.doi.org/10.1080/20013078.2018.1463779Extracellular vesiclesT. cruzi host interaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kleber Silva Ribeiro Camilla Ioshida Vasconcellos Rodrigo Pedro Soares Maria Tays Mendes Cameron C. Ellis Marcela Aguilera-Flores Igor Correia de Almeida Sergio Schenkman Leo Kei Iwai Ana Claudia Torrecilhas |
spellingShingle |
Kleber Silva Ribeiro Camilla Ioshida Vasconcellos Rodrigo Pedro Soares Maria Tays Mendes Cameron C. Ellis Marcela Aguilera-Flores Igor Correia de Almeida Sergio Schenkman Leo Kei Iwai Ana Claudia Torrecilhas Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells Journal of Extracellular Vesicles Extracellular vesicles T. cruzi host interaction |
author_facet |
Kleber Silva Ribeiro Camilla Ioshida Vasconcellos Rodrigo Pedro Soares Maria Tays Mendes Cameron C. Ellis Marcela Aguilera-Flores Igor Correia de Almeida Sergio Schenkman Leo Kei Iwai Ana Claudia Torrecilhas |
author_sort |
Kleber Silva Ribeiro |
title |
Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells |
title_short |
Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells |
title_full |
Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells |
title_fullStr |
Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells |
title_full_unstemmed |
Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells |
title_sort |
proteomic analysis reveals different composition of extracellular vesicles released by two trypanosoma cruzi strains associated with their distinct interaction with host cells |
publisher |
Taylor & Francis Group |
series |
Journal of Extracellular Vesicles |
issn |
2001-3078 |
publishDate |
2018-12-01 |
description |
Trypanosoma cruzi, the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi, which were previously shown to differentially modulate host innate and acquired immune responses. Tissue culture-derived trypomastigotes of both strains secreted extracellular vesicles (EVs), as demonstrated by electron scanning microscopy. EVs were purified by exclusion chromatography or ultracentrifugation and quantitated using nanoparticle tracking analysis. Trypomastigotes from YuYu strain released higher number of EVs than those from Y strain, enriched with virulence factors trans-sialidase (TS) and cruzipain. Proteomic analysis confirmed the increased abundance of proteins coded by the TS gene family, mucin-like glycoproteins, and some typical exosomal proteins in the YuYu strain, which also showed considerable differences between purified EVs and vesicle-free fraction as compared to the Y strain. To evaluate whether such differences were related to parasite infectivity, J774 macrophages and LLC-MK2 kidney cells were preincubated with purified EVs from both strains and then infected with Y strain trypomastigotes. EVs released by YuYu strain caused a lower infection but higher intracellular proliferation in J774 macrophages than EVs from Y strain. In contrast, YuYu strain-derived EVs caused higher infection of LLC-MK2 cells than Y strain-derived EVs. In conclusion, quantitative and qualitative differences in EVs and secreted proteins from different T. cruzi strains may correlate with infectivity/virulence during the host–parasite interaction. |
topic |
Extracellular vesicles T. cruzi host interaction |
url |
http://dx.doi.org/10.1080/20013078.2018.1463779 |
work_keys_str_mv |
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