The effect of changes in nutritional components and environmental factors on growth pattern and biofilm formation by Mycobacterium marinum CCUG 20998
Introduction: Mycobacterium marinum is a slow growing nontuberculosis mycobacterium. This organism can cause disseminated granulomatous infections in fish, called ‘fish tuberculosis’. Changes in nutritional components in culture medium and environmental conditions may lead to some...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
University of Isfahan
2015-06-01
|
Series: | Biological Journal of Microorganism |
Subjects: | |
Online Access: | http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-443-1&slc_lang=en&sid=1 |
Summary: | Introduction: Mycobacterium marinum is a slow growing
nontuberculosis mycobacterium. This organism can cause disseminated
granulomatous infections in fish, called ‘fish tuberculosis’. Changes in
nutritional components in culture medium and environmental conditions may lead
to some changes in growth patterns and biofilm formation having new
characteristics. The aim of this study was the evaluation of changes in medium
components and environmental factors on the growth pattern and biofilm
formation of M. marinum CCUG 20998. Materials and methods: M. marinum CCUG 20998 was grown in 7H9
broth medium containing different concentrations of Tween 80 (0.1, 0.2, 0.3,
0.4, 0.5 and 0.6 % v/v), Oleic Albumin Dextrose Catalase supplement (OADC), (4,
8, 12, and 16 % v/v) and glycerol (30, 35, 40, 45, and 50 % v/v). The changes
in growth patterns and biofilm formation were determined by using measurement
of optical density and staining by crystal violet. Results: The obtained results
showed that maximum growth was at 0.4 % of Tween 80. Oleic Albumin Dextrose
Catalase supplement at different concentrations did not show any significant
effect on the growth pattern. Decreasing the glycerol concentration
significantly decreased the growth rate. The maximum biofilm formation was
obtained in 35 % glycerol without Tween 80 and OADC in culture medium. Discussion and conclusion: According to the obtained results, Tween 80 and glycerol in the
formulation of Middlebrook 7H9 Broth are very critical for M. marinum
CCUG 20998 growth. In contrast, Oleic Albumin Dextrose Catalase supplement can
be omitted from the medium formulation due to showing no significant effect on
the growth pattern. |
---|---|
ISSN: | 2322-5173 2322-5181 |