Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice
Abstract Yogurt from Xinjiang, China, is a traditional Chinese fermented food rich in beneficial microorganisms, such as Lactobacillus plantarum KSFY06. In this study, the effect of KSFY06 on oxidative aging was investigated using live animal experiments. Molecular biological methods were used to an...
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doaj-7727fe77875a4791be67074712eec1ee2020-11-25T02:10:44ZengWileyFood Science & Nutrition2048-71772020-01-018137938910.1002/fsn3.1318Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming miceFang Li0Guangbin Huang1Fang Tan2Ruokun Yi3Xianrong Zhou4Jianfei Mu5Xin Zhao6Chongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing ChinaDepartment of Trauma Surgery Emergency Medical Center of Chongqing The Affiliated Central Hospital of Chongqing University Chongqing ChinaDepartment of Public Health Our Lady of Fatima University Valenzuela PhilippinesChongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing ChinaChongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing ChinaChongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing ChinaChongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing ChinaAbstract Yogurt from Xinjiang, China, is a traditional Chinese fermented food rich in beneficial microorganisms, such as Lactobacillus plantarum KSFY06. In this study, the effect of KSFY06 on oxidative aging was investigated using live animal experiments. Molecular biological methods were used to analyze the serum and tissues of mice with oxidative aging induced by d‐galactose, which showed that KSFY06 can inhibit the decline of heart, liver, spleen, and kidney caused by aging. The KSFY06 strain increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), catalase (CAT), and glutathione (GSH) in serum and liver of aging mice, while the content of malondialdehyde (MDA) is reduced. Pathological observation showed that KSFY06 alleviated damage to the liver, spleen, and skin of oxidative aging mice. qPCR showed that, at high dose (2 × 109 cfu/kg per day), KSFY06 upregulates copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), catalase (CAT) mRNA expression, and its downstream inducible nitric oxide synthase (iNOS) mRNA expression in liver and spleen tissues induced by d‐gal. To a certain extent, these findings indicate that L. plantarum KSFY06 is able to protect against oxidative stress in the d‐gal‐induced aging model. In conclusion, L. plantarum KSFY06 may provide a potential research value in the prevention or alleviation of related diseases caused by oxidative stress.https://doi.org/10.1002/fsn3.1318agingLactobacillus plantarum KSFY06miceoxidation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fang Li Guangbin Huang Fang Tan Ruokun Yi Xianrong Zhou Jianfei Mu Xin Zhao |
spellingShingle |
Fang Li Guangbin Huang Fang Tan Ruokun Yi Xianrong Zhou Jianfei Mu Xin Zhao Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice Food Science & Nutrition aging Lactobacillus plantarum KSFY06 mice oxidation |
author_facet |
Fang Li Guangbin Huang Fang Tan Ruokun Yi Xianrong Zhou Jianfei Mu Xin Zhao |
author_sort |
Fang Li |
title |
Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice |
title_short |
Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice |
title_full |
Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice |
title_fullStr |
Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice |
title_full_unstemmed |
Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice |
title_sort |
lactobacillus plantarum ksfy06 on d‐galactose‐induced oxidation and aging in kunming mice |
publisher |
Wiley |
series |
Food Science & Nutrition |
issn |
2048-7177 |
publishDate |
2020-01-01 |
description |
Abstract Yogurt from Xinjiang, China, is a traditional Chinese fermented food rich in beneficial microorganisms, such as Lactobacillus plantarum KSFY06. In this study, the effect of KSFY06 on oxidative aging was investigated using live animal experiments. Molecular biological methods were used to analyze the serum and tissues of mice with oxidative aging induced by d‐galactose, which showed that KSFY06 can inhibit the decline of heart, liver, spleen, and kidney caused by aging. The KSFY06 strain increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), catalase (CAT), and glutathione (GSH) in serum and liver of aging mice, while the content of malondialdehyde (MDA) is reduced. Pathological observation showed that KSFY06 alleviated damage to the liver, spleen, and skin of oxidative aging mice. qPCR showed that, at high dose (2 × 109 cfu/kg per day), KSFY06 upregulates copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), catalase (CAT) mRNA expression, and its downstream inducible nitric oxide synthase (iNOS) mRNA expression in liver and spleen tissues induced by d‐gal. To a certain extent, these findings indicate that L. plantarum KSFY06 is able to protect against oxidative stress in the d‐gal‐induced aging model. In conclusion, L. plantarum KSFY06 may provide a potential research value in the prevention or alleviation of related diseases caused by oxidative stress. |
topic |
aging Lactobacillus plantarum KSFY06 mice oxidation |
url |
https://doi.org/10.1002/fsn3.1318 |
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