miRNAs as radio‐response biomarkers for breast cancer stem cells
In breast cancer (BC), the presence of cancer stem cells (CSCs) has been related to relapse, metastasis, and radioresistance. Radiotherapy (RT) is an extended BC treatment, but is not always effective. CSCs have several mechanisms of radioresistance in place, and some miRNAs are involved in the cell...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wiley
2020-03-01
|
Series: | Molecular Oncology |
Subjects: | |
Online Access: | https://doi.org/10.1002/1878-0261.12635 |
id |
doaj-772894d2de15446087d8be1b59bd8801 |
---|---|
record_format |
Article |
spelling |
doaj-772894d2de15446087d8be1b59bd88012020-11-25T03:43:04ZengWileyMolecular Oncology1574-78911878-02612020-03-0114355657010.1002/1878-0261.12635miRNAs as radio‐response biomarkers for breast cancer stem cellsCarmen Griñán‐Lisón0María Auxiliadora Olivares‐Urbano1Gema Jiménez2Elena López‐Ruiz3Coral delVal4Cynthia Morata‐Tarifa5José Manuel Entrena6Amanda Rocío González‐Ramírez7Houria Boulaiz8Mercedes Zurita Herrera9María Isabel Núñez10Juan Antonio Marchal11Biopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research (CIBM) University of Granada Granada SpainDepartment of Radiology and Physical Medicine University of Granada SpainBiopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research (CIBM) University of Granada Granada SpainBiopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research (CIBM) University of Granada Granada SpainDepartment of Artificial Intelligence University of Granada SpainBiopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research (CIBM) University of Granada Granada SpainBiopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research (CIBM) University of Granada Granada SpainInstituto de Investigación Biosanitaria ibs.GRANADA SpainBiopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research (CIBM) University of Granada Granada SpainRadiation Oncology Department Virgen de las Nieves University Hospital Granada SpainBiopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research (CIBM) University of Granada Granada SpainBiopathology and Regenerative Medicine Institute (IBIMER) Centre for Biomedical Research (CIBM) University of Granada Granada SpainIn breast cancer (BC), the presence of cancer stem cells (CSCs) has been related to relapse, metastasis, and radioresistance. Radiotherapy (RT) is an extended BC treatment, but is not always effective. CSCs have several mechanisms of radioresistance in place, and some miRNAs are involved in the cellular response to ionizing radiation (IR). Here, we studied how IR affects the expression of miRNAs related to stemness in different molecular BC subtypes. Exposition of BC cells to radiation doses of 2, 4, or 6 Gy affected their phenotype, functional characteristics, pluripotency gene expression, and in vivo tumorigenic capacity. This held true for various molecular subtypes of BC cells (classified by ER, PR and HER‐2 status), and for BC cells either plated in monolayer, or being in suspension as mammospheres. However, the effect of IR on the expression of eight stemness‐ and radioresistance‐related miRNAs (miR‐210, miR‐10b, miR‐182, miR‐142, miR‐221, miR‐21, miR‐93, miR‐15b) varied, depending on cell line subpopulation and clinicopathological features of BC patients. Therefore, clinicopathological features and, potentially also, chemotherapy regimen should be both taken into consideration, for determining a potential miRNA signature by liquid biopsy in BC patients treated with RT. Personalized and precision RT dosage regimes could improve the prognosis, treatment, and survival of BC patients.https://doi.org/10.1002/1878-0261.12635biomarkersbreast cancerCSCsmiRNAsradiationradiotherapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carmen Griñán‐Lisón María Auxiliadora Olivares‐Urbano Gema Jiménez Elena López‐Ruiz Coral delVal Cynthia Morata‐Tarifa José Manuel Entrena Amanda Rocío González‐Ramírez Houria Boulaiz Mercedes Zurita Herrera María Isabel Núñez Juan Antonio Marchal |
spellingShingle |
Carmen Griñán‐Lisón María Auxiliadora Olivares‐Urbano Gema Jiménez Elena López‐Ruiz Coral delVal Cynthia Morata‐Tarifa José Manuel Entrena Amanda Rocío González‐Ramírez Houria Boulaiz Mercedes Zurita Herrera María Isabel Núñez Juan Antonio Marchal miRNAs as radio‐response biomarkers for breast cancer stem cells Molecular Oncology biomarkers breast cancer CSCs miRNAs radiation radiotherapy |
author_facet |
Carmen Griñán‐Lisón María Auxiliadora Olivares‐Urbano Gema Jiménez Elena López‐Ruiz Coral delVal Cynthia Morata‐Tarifa José Manuel Entrena Amanda Rocío González‐Ramírez Houria Boulaiz Mercedes Zurita Herrera María Isabel Núñez Juan Antonio Marchal |
author_sort |
Carmen Griñán‐Lisón |
title |
miRNAs as radio‐response biomarkers for breast cancer stem cells |
title_short |
miRNAs as radio‐response biomarkers for breast cancer stem cells |
title_full |
miRNAs as radio‐response biomarkers for breast cancer stem cells |
title_fullStr |
miRNAs as radio‐response biomarkers for breast cancer stem cells |
title_full_unstemmed |
miRNAs as radio‐response biomarkers for breast cancer stem cells |
title_sort |
mirnas as radio‐response biomarkers for breast cancer stem cells |
publisher |
Wiley |
series |
Molecular Oncology |
issn |
1574-7891 1878-0261 |
publishDate |
2020-03-01 |
description |
In breast cancer (BC), the presence of cancer stem cells (CSCs) has been related to relapse, metastasis, and radioresistance. Radiotherapy (RT) is an extended BC treatment, but is not always effective. CSCs have several mechanisms of radioresistance in place, and some miRNAs are involved in the cellular response to ionizing radiation (IR). Here, we studied how IR affects the expression of miRNAs related to stemness in different molecular BC subtypes. Exposition of BC cells to radiation doses of 2, 4, or 6 Gy affected their phenotype, functional characteristics, pluripotency gene expression, and in vivo tumorigenic capacity. This held true for various molecular subtypes of BC cells (classified by ER, PR and HER‐2 status), and for BC cells either plated in monolayer, or being in suspension as mammospheres. However, the effect of IR on the expression of eight stemness‐ and radioresistance‐related miRNAs (miR‐210, miR‐10b, miR‐182, miR‐142, miR‐221, miR‐21, miR‐93, miR‐15b) varied, depending on cell line subpopulation and clinicopathological features of BC patients. Therefore, clinicopathological features and, potentially also, chemotherapy regimen should be both taken into consideration, for determining a potential miRNA signature by liquid biopsy in BC patients treated with RT. Personalized and precision RT dosage regimes could improve the prognosis, treatment, and survival of BC patients. |
topic |
biomarkers breast cancer CSCs miRNAs radiation radiotherapy |
url |
https://doi.org/10.1002/1878-0261.12635 |
work_keys_str_mv |
AT carmengrinanlison mirnasasradioresponsebiomarkersforbreastcancerstemcells AT mariaauxiliadoraolivaresurbano mirnasasradioresponsebiomarkersforbreastcancerstemcells AT gemajimenez mirnasasradioresponsebiomarkersforbreastcancerstemcells AT elenalopezruiz mirnasasradioresponsebiomarkersforbreastcancerstemcells AT coraldelval mirnasasradioresponsebiomarkersforbreastcancerstemcells AT cynthiamoratatarifa mirnasasradioresponsebiomarkersforbreastcancerstemcells AT josemanuelentrena mirnasasradioresponsebiomarkersforbreastcancerstemcells AT amandarociogonzalezramirez mirnasasradioresponsebiomarkersforbreastcancerstemcells AT houriaboulaiz mirnasasradioresponsebiomarkersforbreastcancerstemcells AT mercedeszuritaherrera mirnasasradioresponsebiomarkersforbreastcancerstemcells AT mariaisabelnunez mirnasasradioresponsebiomarkersforbreastcancerstemcells AT juanantoniomarchal mirnasasradioresponsebiomarkersforbreastcancerstemcells |
_version_ |
1724521471995281408 |