Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers
This study analyzed the genetic diversity and structure of two Bulgarian indigenous sheep (Ovis aries) breeds – Kotel and Teteven, each presented by 4 flocks, using 15 microsatellite markers. A total of 195 alleles were identified. Among the markers used, the most informative were INRA5 and OarFCB20...
Main Author: | |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2021-01-01
|
Series: | Biotechnology & Biotechnological Equipment |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/13102818.2021.1903339 |
id |
doaj-773f681502954e94bb74b6e314195f38 |
---|---|
record_format |
Article |
spelling |
doaj-773f681502954e94bb74b6e314195f382021-04-21T16:14:21ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302021-01-0135157658510.1080/13102818.2021.19033391903339Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markersYanka Mihailova0Department of Animal Science, Faculty of Agronomy, Agricultural University PlovdivThis study analyzed the genetic diversity and structure of two Bulgarian indigenous sheep (Ovis aries) breeds – Kotel and Teteven, each presented by 4 flocks, using 15 microsatellite markers. A total of 195 alleles were identified. Among the markers used, the most informative were INRA5 and OarFCB20 with PIC values of 0.87. Both breeds showed a high level of genetic diversity with average values of He and Ho, respectively 0.74 and 0.76. The observed high level of genetic diversity is combined with the absence of heterozygous deficiency reported as Fis values of 0.04 and 0.07, respectively for Kotel and Teteven breeds. Low level of genetic differentiation between the two breeds was observed, as evidenced by the low value of Fst of 0.09, and analysis of variance (ANOVA) data showing that only 9% of the total variation is due to variation between breeds, whereas 91% is due to variation within breeds. Furthermore, we revealed a breeding practice that comprises an exchange of animals between the breeds which reflects in the clustering obtained by STRUCTURE analysis. According to it, Kotel breed is a more homogenous population and forms cluster 1, whereas Teteven breed is more heterogenous and consists of two subpopulations that form two distinct clusters. The results obtained in the present study confirm the high potential of microsatellite markers in studies related to both breed affiliation and purity and can serve as a basis for the implementation of future management and conservation programs of these indigenous breeds.http://dx.doi.org/10.1080/13102818.2021.1903339sheep breeds (o. aries)microsatellite markers (ssr)genetic diversitygenetic structure |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanka Mihailova |
spellingShingle |
Yanka Mihailova Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers Biotechnology & Biotechnological Equipment sheep breeds (o. aries) microsatellite markers (ssr) genetic diversity genetic structure |
author_facet |
Yanka Mihailova |
author_sort |
Yanka Mihailova |
title |
Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers |
title_short |
Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers |
title_full |
Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers |
title_fullStr |
Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers |
title_full_unstemmed |
Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers |
title_sort |
genetic diversity and structure of 2 indigenous sheep breeds (kotel and teteven) in bulgaria using microsatellite markers |
publisher |
Taylor & Francis Group |
series |
Biotechnology & Biotechnological Equipment |
issn |
1310-2818 1314-3530 |
publishDate |
2021-01-01 |
description |
This study analyzed the genetic diversity and structure of two Bulgarian indigenous sheep (Ovis aries) breeds – Kotel and Teteven, each presented by 4 flocks, using 15 microsatellite markers. A total of 195 alleles were identified. Among the markers used, the most informative were INRA5 and OarFCB20 with PIC values of 0.87. Both breeds showed a high level of genetic diversity with average values of He and Ho, respectively 0.74 and 0.76. The observed high level of genetic diversity is combined with the absence of heterozygous deficiency reported as Fis values of 0.04 and 0.07, respectively for Kotel and Teteven breeds. Low level of genetic differentiation between the two breeds was observed, as evidenced by the low value of Fst of 0.09, and analysis of variance (ANOVA) data showing that only 9% of the total variation is due to variation between breeds, whereas 91% is due to variation within breeds. Furthermore, we revealed a breeding practice that comprises an exchange of animals between the breeds which reflects in the clustering obtained by STRUCTURE analysis. According to it, Kotel breed is a more homogenous population and forms cluster 1, whereas Teteven breed is more heterogenous and consists of two subpopulations that form two distinct clusters. The results obtained in the present study confirm the high potential of microsatellite markers in studies related to both breed affiliation and purity and can serve as a basis for the implementation of future management and conservation programs of these indigenous breeds. |
topic |
sheep breeds (o. aries) microsatellite markers (ssr) genetic diversity genetic structure |
url |
http://dx.doi.org/10.1080/13102818.2021.1903339 |
work_keys_str_mv |
AT yankamihailova geneticdiversityandstructureof2indigenoussheepbreedskotelandteteveninbulgariausingmicrosatellitemarkers |
_version_ |
1721516113193336832 |