Micropropagation of Micromeria juliana (L.) Benth. ex Rchb. (Lamiaceae)

• Main Authors: Tošić, S., Nikolić, S., Jovanović, M., Zlatković, B., Stojičić, D.
• Format: Article
• Language: English
• Published: University of Niš 2015-09-01
• Series: Biologica Nyssana
• Subjects: axillary bud induction; shoot culture; biomass production
• Online Access: http://journal.pmf.ni.ac.rs/bionys/index.php/bionys/article/view/127/87

Micromeria juliana belongs to family Lamiaceae, whose representatives are characterized by a significant level of essential oils and antioxidant components. Several species of genus Micromeria contain biologically active substances and are therefore used in folk medicine, food industry and cosmetic industry. Methods of their tissue culture may provide rapid mass multiplication of plants for various purposes, including research on production, accumulation and metabolism of important secondary metabolites. The goal of this paper was to determine the protocol for regeneration of M. juliana plants through use of nodal explants on nutritive substrate with various growth regulators. The greatest number of axillary buds was formed in explants grown on MS nutritive medium with 3 μM benzyladenine (BA) and 0.57 μM indole-3-acetic acid (IAA). The explants grown at the medium without any growth regulators and the medium with auxin have shown spontaneous root formation.