Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells

The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of...

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Main Authors:	Bo-jiang Li, Ping-hua Li, Rui-hua Huang, Wen-xing Sun, Han Wang, Qi-fa Li, Jie Chen, Wang-jun Wu, Hong-lin Liu
Format:	Article
Language:	English
Published:	Asian-Australasian Association of Animal Production Societies 2015-08-01
Series:	Asian-Australasian Journal of Animal Sciences
Subjects:	Pig Skeletal Muscle Satellite Cell Isolation Culture Characterization
Online Access:	http://www.ajas.info/upload/pdf/ajas-28-8-1171.pdf

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spelling	doaj-776186290ed5432293d003921c43c87f2020-11-25T00:06:34ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172015-08-012881171117710.5713/ajas.14.084823244Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite CellsBo-jiang Li0Ping-hua Li1Rui-hua Huang2Wen-xing Sun3Han Wang4Qi-fa Li5Jie Chen6Wang-jun Wu7Hong-lin Liu8 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaThe objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.http://www.ajas.info/upload/pdf/ajas-28-8-1171.pdfPigSkeletal Muscle Satellite CellIsolationCultureCharacterization
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Bo-jiang Li Ping-hua Li Rui-hua Huang Wen-xing Sun Han Wang Qi-fa Li Jie Chen Wang-jun Wu Hong-lin Liu
spellingShingle	Bo-jiang Li Ping-hua Li Rui-hua Huang Wen-xing Sun Han Wang Qi-fa Li Jie Chen Wang-jun Wu Hong-lin Liu Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells Asian-Australasian Journal of Animal Sciences Pig Skeletal Muscle Satellite Cell Isolation Culture Characterization
author_facet	Bo-jiang Li Ping-hua Li Rui-hua Huang Wen-xing Sun Han Wang Qi-fa Li Jie Chen Wang-jun Wu Hong-lin Liu
author_sort	Bo-jiang Li
title	Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
title_short	Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
title_full	Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
title_fullStr	Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
title_full_unstemmed	Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
title_sort	isolation, culture and identification of porcine skeletal muscle satellite cells
publisher	Asian-Australasian Association of Animal Production Societies
series	Asian-Australasian Journal of Animal Sciences
issn	1011-2367 1976-5517
publishDate	2015-08-01
description	The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.
topic	Pig Skeletal Muscle Satellite Cell Isolation Culture Characterization
url	http://www.ajas.info/upload/pdf/ajas-28-8-1171.pdf
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Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells

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