Structural Basis of the Substrate Specificity and Enzyme Catalysis of a Papaver somniferum Tyrosine Decarboxylase
Tyrosine decarboxylase (TyDC), a type II pyridoxal 5′-phosphate decarboxylase, catalyzes the decarboxylation of tyrosine. Due to a generally high sequence identity to other aromatic amino acid decarboxylases (AAADs), primary sequence information is not enough to understand substrate specificities wi...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2017-02-01
|
Series: | Frontiers in Molecular Biosciences |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fmolb.2017.00005/full |
id |
doaj-77622dba21464275a251911f8d417ef8 |
---|---|
record_format |
Article |
spelling |
doaj-77622dba21464275a251911f8d417ef82020-11-24T22:16:53ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2017-02-01410.3389/fmolb.2017.00005247298Structural Basis of the Substrate Specificity and Enzyme Catalysis of a Papaver somniferum Tyrosine DecarboxylaseHuai Guan0Huai Guan1Huai Guan2Shuaibao Song3Shuaibao Song4Shuaibao Song5Howard Robinson6Jing Liang7Haizhen Ding8Jianyong Li9Qian Han10Qian Han11Qian Han12Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan UniversityHainan, ChinaHainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityHainan, ChinaLaboratory of Tropical Veterinary Medicine and Vector Biology, Hainan UniversityHaikou, Hainan, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education, Hainan UniversityHainan, ChinaHainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityHainan, ChinaLaboratory of Tropical Veterinary Medicine and Vector Biology, Hainan UniversityHaikou, Hainan, ChinaBiology Department, Brookhaven National Laboratory, UptonNew York, NY, USADepartment of Biochemistry, Virginia TechBlacksburg, VA, USADepartment of Biochemistry, Virginia TechBlacksburg, VA, USADepartment of Biochemistry, Virginia TechBlacksburg, VA, USAKey Laboratory of Tropical Biological Resources of Ministry of Education, Hainan UniversityHainan, ChinaHainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityHainan, ChinaLaboratory of Tropical Veterinary Medicine and Vector Biology, Hainan UniversityHaikou, Hainan, ChinaTyrosine decarboxylase (TyDC), a type II pyridoxal 5′-phosphate decarboxylase, catalyzes the decarboxylation of tyrosine. Due to a generally high sequence identity to other aromatic amino acid decarboxylases (AAADs), primary sequence information is not enough to understand substrate specificities with structural information. In this study, we selected a typical TyDC from Papaver somniferum as a model to study the structural basis of AAAD substrate specificities. Analysis of the native P. somniferum TyDC crystal structure and subsequent molecular docking and dynamics simulation provide some structural bases that explain substrate specificity for tyrosine. The result confirmed the previous proposed mechanism for the enzyme selectivity of indolic and phenolic substrates. Additionally, this study yields the first crystal structure for a plant type II pyridoxal-5'-phosphate decarboxylase.http://journal.frontiersin.org/article/10.3389/fmolb.2017.00005/fullaromatic amino acid decarboxylasetyrosine decarboxylasesdecarboxylasecrystal structurePapaver somniferumsubstrate specificity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huai Guan Huai Guan Huai Guan Shuaibao Song Shuaibao Song Shuaibao Song Howard Robinson Jing Liang Haizhen Ding Jianyong Li Qian Han Qian Han Qian Han |
spellingShingle |
Huai Guan Huai Guan Huai Guan Shuaibao Song Shuaibao Song Shuaibao Song Howard Robinson Jing Liang Haizhen Ding Jianyong Li Qian Han Qian Han Qian Han Structural Basis of the Substrate Specificity and Enzyme Catalysis of a Papaver somniferum Tyrosine Decarboxylase Frontiers in Molecular Biosciences aromatic amino acid decarboxylase tyrosine decarboxylases decarboxylase crystal structure Papaver somniferum substrate specificity |
author_facet |
Huai Guan Huai Guan Huai Guan Shuaibao Song Shuaibao Song Shuaibao Song Howard Robinson Jing Liang Haizhen Ding Jianyong Li Qian Han Qian Han Qian Han |
author_sort |
Huai Guan |
title |
Structural Basis of the Substrate Specificity and Enzyme Catalysis of a Papaver somniferum Tyrosine Decarboxylase |
title_short |
Structural Basis of the Substrate Specificity and Enzyme Catalysis of a Papaver somniferum Tyrosine Decarboxylase |
title_full |
Structural Basis of the Substrate Specificity and Enzyme Catalysis of a Papaver somniferum Tyrosine Decarboxylase |
title_fullStr |
Structural Basis of the Substrate Specificity and Enzyme Catalysis of a Papaver somniferum Tyrosine Decarboxylase |
title_full_unstemmed |
Structural Basis of the Substrate Specificity and Enzyme Catalysis of a Papaver somniferum Tyrosine Decarboxylase |
title_sort |
structural basis of the substrate specificity and enzyme catalysis of a papaver somniferum tyrosine decarboxylase |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Biosciences |
issn |
2296-889X |
publishDate |
2017-02-01 |
description |
Tyrosine decarboxylase (TyDC), a type II pyridoxal 5′-phosphate decarboxylase, catalyzes the decarboxylation of tyrosine. Due to a generally high sequence identity to other aromatic amino acid decarboxylases (AAADs), primary sequence information is not enough to understand substrate specificities with structural information. In this study, we selected a typical TyDC from Papaver somniferum as a model to study the structural basis of AAAD substrate specificities. Analysis of the native P. somniferum TyDC crystal structure and subsequent molecular docking and dynamics simulation provide some structural bases that explain substrate specificity for tyrosine. The result confirmed the previous proposed mechanism for the enzyme selectivity of indolic and phenolic substrates. Additionally, this study yields the first crystal structure for a plant type II pyridoxal-5'-phosphate decarboxylase. |
topic |
aromatic amino acid decarboxylase tyrosine decarboxylases decarboxylase crystal structure Papaver somniferum substrate specificity |
url |
http://journal.frontiersin.org/article/10.3389/fmolb.2017.00005/full |
work_keys_str_mv |
AT huaiguan structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT huaiguan structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT huaiguan structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT shuaibaosong structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT shuaibaosong structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT shuaibaosong structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT howardrobinson structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT jingliang structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT haizhending structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT jianyongli structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT qianhan structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT qianhan structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase AT qianhan structuralbasisofthesubstratespecificityandenzymecatalysisofapapaversomniferumtyrosinedecarboxylase |
_version_ |
1725787864296325120 |