Enterovirus 71 viral capsid protein linear epitopes: Identification and characterization
<p>Abstract</p> <p>Background</p> <p>To characterize the human humoral immune response against enterovirus 71 (EV71) infection and map human epitopes on the viral capsid proteins.</p> <p>Methods</p> <p>A series of 256 peptides spanning the capsid...
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doaj-7766e164d02c41d882f193ea2a8636d22020-11-25T00:18:55ZengBMCVirology Journal1743-422X2012-01-01912610.1186/1743-422X-9-26Enterovirus 71 viral capsid protein linear epitopes: Identification and characterizationGao FanWang Yi-PingMao Qun-YingYao XinLiu ShuangLi Feng-XiangZhu Feng-CaiYang Jing-YuLiang Zheng-LunLu Feng-MinWang Jun-Zhi<p>Abstract</p> <p>Background</p> <p>To characterize the human humoral immune response against enterovirus 71 (EV71) infection and map human epitopes on the viral capsid proteins.</p> <p>Methods</p> <p>A series of 256 peptides spanning the capsid proteins (VP1, VP2, VP3) of BJ08 strain (genomic C4) were synthesized. An indirect enzyme-linked immunosorbent assay (ELISA) was carried out to detect anti-EV71 IgM and IgG in sera of infected children in acute or recovery phase. The partially overlapped peptides contained 12 amino acids and were coated in the plate as antigen (0.1 μg/μl). Sera from rabbits immunized with inactivated BJ08 virus were also used to screen the peptide panel.</p> <p>Results</p> <p>A total of 10 human anti-EV71 IgM epitopes (vp1-14 in VP1; vp2-6, 21, 40 and 50 in VP2 and vp3-10, 12, 15, 24 and 75 in VP3) were identified in acute phase sera. In contrast, only one anti-EV71 IgG epitope in VP1 (vp1-15) was identified in sera of recovery stage. Four rabbit anti-EV71 IgG epitopes (vp1-14, 31, 54 and 71) were identified and mapped to VP1.</p> <p>Conclusion</p> <p>These data suggested that human IgM epitopes were mainly mapped to VP2 and VP3 with multi-epitope responses occurred at acute infection, while the only IgG epitope located on protein VP1 was activated in recovery phase sera. The dynamic changes of humoral immune response at different stages of infection may have public health significance in evaluation of EV71 vaccine immunogenicity and the clinical application of diagnostic reagents.</p> http://www.virologyj.com/content/9/1/26EV71Capsid proteinEpitopesHumoral immune response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gao Fan Wang Yi-Ping Mao Qun-Ying Yao Xin Liu Shuang Li Feng-Xiang Zhu Feng-Cai Yang Jing-Yu Liang Zheng-Lun Lu Feng-Min Wang Jun-Zhi |
spellingShingle |
Gao Fan Wang Yi-Ping Mao Qun-Ying Yao Xin Liu Shuang Li Feng-Xiang Zhu Feng-Cai Yang Jing-Yu Liang Zheng-Lun Lu Feng-Min Wang Jun-Zhi Enterovirus 71 viral capsid protein linear epitopes: Identification and characterization Virology Journal EV71 Capsid protein Epitopes Humoral immune response |
author_facet |
Gao Fan Wang Yi-Ping Mao Qun-Ying Yao Xin Liu Shuang Li Feng-Xiang Zhu Feng-Cai Yang Jing-Yu Liang Zheng-Lun Lu Feng-Min Wang Jun-Zhi |
author_sort |
Gao Fan |
title |
Enterovirus 71 viral capsid protein linear epitopes: Identification and characterization |
title_short |
Enterovirus 71 viral capsid protein linear epitopes: Identification and characterization |
title_full |
Enterovirus 71 viral capsid protein linear epitopes: Identification and characterization |
title_fullStr |
Enterovirus 71 viral capsid protein linear epitopes: Identification and characterization |
title_full_unstemmed |
Enterovirus 71 viral capsid protein linear epitopes: Identification and characterization |
title_sort |
enterovirus 71 viral capsid protein linear epitopes: identification and characterization |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2012-01-01 |
description |
<p>Abstract</p> <p>Background</p> <p>To characterize the human humoral immune response against enterovirus 71 (EV71) infection and map human epitopes on the viral capsid proteins.</p> <p>Methods</p> <p>A series of 256 peptides spanning the capsid proteins (VP1, VP2, VP3) of BJ08 strain (genomic C4) were synthesized. An indirect enzyme-linked immunosorbent assay (ELISA) was carried out to detect anti-EV71 IgM and IgG in sera of infected children in acute or recovery phase. The partially overlapped peptides contained 12 amino acids and were coated in the plate as antigen (0.1 μg/μl). Sera from rabbits immunized with inactivated BJ08 virus were also used to screen the peptide panel.</p> <p>Results</p> <p>A total of 10 human anti-EV71 IgM epitopes (vp1-14 in VP1; vp2-6, 21, 40 and 50 in VP2 and vp3-10, 12, 15, 24 and 75 in VP3) were identified in acute phase sera. In contrast, only one anti-EV71 IgG epitope in VP1 (vp1-15) was identified in sera of recovery stage. Four rabbit anti-EV71 IgG epitopes (vp1-14, 31, 54 and 71) were identified and mapped to VP1.</p> <p>Conclusion</p> <p>These data suggested that human IgM epitopes were mainly mapped to VP2 and VP3 with multi-epitope responses occurred at acute infection, while the only IgG epitope located on protein VP1 was activated in recovery phase sera. The dynamic changes of humoral immune response at different stages of infection may have public health significance in evaluation of EV71 vaccine immunogenicity and the clinical application of diagnostic reagents.</p> |
topic |
EV71 Capsid protein Epitopes Humoral immune response |
url |
http://www.virologyj.com/content/9/1/26 |
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