Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts

Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts

Myoblast activation is a triggering event for muscle remodeling. We assessed the stimulatory effects of propolis, a beehive product, on myoblasts. After an 8 h treatment with 100 μg/mL of Brazilian propolis ethanol extract, expression of various chemokines, including CCL-2 and CCL-5, and cytokines,...

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Main Authors: Kohei Washio, Mao Kobayashi, Natsuko Saito, Misato Amagasa, Hiroshi Kitamura
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:Evidence-Based Complementary and Alternative Medicine
Online Access:http://dx.doi.org/10.1155/2015/349751
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spelling doaj-77af445b5443479b80ba574feb845ac32020-11-25T00:07:13ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882015-01-01201510.1155/2015/349751349751Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 MyoblastsKohei Washio0Mao Kobayashi1Natsuko Saito2Misato Amagasa3Hiroshi Kitamura4Laboratory for Veterinary Physiology, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu 069-8501, JapanLaboratory for Veterinary Physiology, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu 069-8501, JapanLaboratory for Veterinary Physiology, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu 069-8501, JapanLaboratory for Veterinary Physiology, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu 069-8501, JapanLaboratory for Veterinary Physiology, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu 069-8501, JapanMyoblast activation is a triggering event for muscle remodeling. We assessed the stimulatory effects of propolis, a beehive product, on myoblasts. After an 8 h treatment with 100 μg/mL of Brazilian propolis ethanol extract, expression of various chemokines, including CCL-2 and CCL-5, and cytokines, such as IL-6, increased. This propolis-induced cytokine production appears to depend on NF-κB activation, because the IKK inhibitor BMS-345541 repressed mRNA levels of CCL-2 by ~66%, CCL-5 by ~81%, and IL-6 by ~69% after propolis treatment. Supernatant from propolis-conditioned C2C12 cells upregulated RAW264 macrophage migration. The supernatant also stimulated RAW264 cells to produce angiogenic factors, including VEGF-A and MMP-12. Brazilian green propolis therefore causes myoblasts to secrete cytokines and chemokines, which might contribute to tissue remodeling of skeletal muscle.http://dx.doi.org/10.1155/2015/349751
collection DOAJ
language English
format Article
sources DOAJ
author Kohei Washio
Mao Kobayashi
Natsuko Saito
Misato Amagasa
Hiroshi Kitamura
spellingShingle Kohei Washio
Mao Kobayashi
Natsuko Saito
Misato Amagasa
Hiroshi Kitamura
Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts
Evidence-Based Complementary and Alternative Medicine
author_facet Kohei Washio
Mao Kobayashi
Natsuko Saito
Misato Amagasa
Hiroshi Kitamura
author_sort Kohei Washio
title Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts
title_short Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts
title_full Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts
title_fullStr Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts
title_full_unstemmed Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts
title_sort propolis ethanol extract stimulates cytokine and chemokine production through nf-κb activation in c2c12 myoblasts
publisher Hindawi Limited
series Evidence-Based Complementary and Alternative Medicine
issn 1741-427X
1741-4288
publishDate 2015-01-01
description Myoblast activation is a triggering event for muscle remodeling. We assessed the stimulatory effects of propolis, a beehive product, on myoblasts. After an 8 h treatment with 100 μg/mL of Brazilian propolis ethanol extract, expression of various chemokines, including CCL-2 and CCL-5, and cytokines, such as IL-6, increased. This propolis-induced cytokine production appears to depend on NF-κB activation, because the IKK inhibitor BMS-345541 repressed mRNA levels of CCL-2 by ~66%, CCL-5 by ~81%, and IL-6 by ~69% after propolis treatment. Supernatant from propolis-conditioned C2C12 cells upregulated RAW264 macrophage migration. The supernatant also stimulated RAW264 cells to produce angiogenic factors, including VEGF-A and MMP-12. Brazilian green propolis therefore causes myoblasts to secrete cytokines and chemokines, which might contribute to tissue remodeling of skeletal muscle.
url http://dx.doi.org/10.1155/2015/349751
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AT natsukosaito propolisethanolextractstimulatescytokineandchemokineproductionthroughnfkbactivationinc2c12myoblasts
AT misatoamagasa propolisethanolextractstimulatescytokineandchemokineproductionthroughnfkbactivationinc2c12myoblasts
AT hiroshikitamura propolisethanolextractstimulatescytokineandchemokineproductionthroughnfkbactivationinc2c12myoblasts
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