Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women
Abstract The aim of the present study is to investigate the functional role of TNF-α single-nucleotide polymorphisms/haplotypes in an association with reproductive tract infections (RTIs) in symptomatic and asymptomatic women. A total of 850 consecutive subjects consisting of 400 cases and 450 healt...
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doaj-77af4cbb5155449f865e0691be50b7ad2021-01-17T12:34:16ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111110.1038/s41598-020-79963-yFunctional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian womenVineeta Sharma0Subash Chandra Sonkar1Pallavi Singhal2Anoop Kumar3Rakesh Kumar Singh4V. G. Ramachandran5Roopa Hariprasad6Daman Saluja7Mausumi Bharadwaj8Division of Molecular Genetics and Biochemistry, National Institute of Cancer Prevention and Research (ICMR)Multidisciplinary Research Unit (MRU), Maulana Azad Medical College, University of DelhiDivision of Molecular Genetics and Biochemistry, National Institute of Cancer Prevention and Research (ICMR)National Institute of BiologicalsDirectorate of Census OperationsDepartment of Microbiology, University College of Medical Science, Delhi UniversityDivision of Clinical Oncology, National Institute of Cancer Prevention and Research (ICMR)Dr. B.R. Ambedkar Center for Biomedical Research (ACBR), University of Delhi (North Campus)Division of Molecular Genetics and Biochemistry, National Institute of Cancer Prevention and Research (ICMR)Abstract The aim of the present study is to investigate the functional role of TNF-α single-nucleotide polymorphisms/haplotypes in an association with reproductive tract infections (RTIs) in symptomatic and asymptomatic women. A total of 850 consecutive subjects consisting of 400 cases and 450 healthy controls, were screened for RTIs, along with their risk factors and associated symptoms. The propensity score matching was performed to reduce the confounding bias arise owing to covariates and to balance the data between two groups. A total of 211 pairs (1:1) have been created. Genotyping of rs1800629 (-308) and rs361525 (-238) SNPs of TNF-α was done by PCR–RFLP followed by sequencing. The functional implication of TNF-α SNPs in an association with RTIs was also checked by using ELISA. The frequency of -238A allele and -308A allele was found to be twofold (P < 0.0001) and threefold (P < 0.0001) higher in the presence of RTIs. AA haplotype emerged as a major player in an association with RTIs and elevated TNF-α expression. The present study revealed the functional role of rs1800629 (-308) and rs361525 (-238) of TNF-α in an association with RTIs. This information may be used to establish biomarkers for an inflammatory response during the persistence of RTIs.https://doi.org/10.1038/s41598-020-79963-y |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vineeta Sharma Subash Chandra Sonkar Pallavi Singhal Anoop Kumar Rakesh Kumar Singh V. G. Ramachandran Roopa Hariprasad Daman Saluja Mausumi Bharadwaj |
spellingShingle |
Vineeta Sharma Subash Chandra Sonkar Pallavi Singhal Anoop Kumar Rakesh Kumar Singh V. G. Ramachandran Roopa Hariprasad Daman Saluja Mausumi Bharadwaj Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women Scientific Reports |
author_facet |
Vineeta Sharma Subash Chandra Sonkar Pallavi Singhal Anoop Kumar Rakesh Kumar Singh V. G. Ramachandran Roopa Hariprasad Daman Saluja Mausumi Bharadwaj |
author_sort |
Vineeta Sharma |
title |
Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women |
title_short |
Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women |
title_full |
Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women |
title_fullStr |
Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women |
title_full_unstemmed |
Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women |
title_sort |
functional impact of allelic variations/haplotypes of tnf-α on reproductive tract infections in indian women |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-01-01 |
description |
Abstract The aim of the present study is to investigate the functional role of TNF-α single-nucleotide polymorphisms/haplotypes in an association with reproductive tract infections (RTIs) in symptomatic and asymptomatic women. A total of 850 consecutive subjects consisting of 400 cases and 450 healthy controls, were screened for RTIs, along with their risk factors and associated symptoms. The propensity score matching was performed to reduce the confounding bias arise owing to covariates and to balance the data between two groups. A total of 211 pairs (1:1) have been created. Genotyping of rs1800629 (-308) and rs361525 (-238) SNPs of TNF-α was done by PCR–RFLP followed by sequencing. The functional implication of TNF-α SNPs in an association with RTIs was also checked by using ELISA. The frequency of -238A allele and -308A allele was found to be twofold (P < 0.0001) and threefold (P < 0.0001) higher in the presence of RTIs. AA haplotype emerged as a major player in an association with RTIs and elevated TNF-α expression. The present study revealed the functional role of rs1800629 (-308) and rs361525 (-238) of TNF-α in an association with RTIs. This information may be used to establish biomarkers for an inflammatory response during the persistence of RTIs. |
url |
https://doi.org/10.1038/s41598-020-79963-y |
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