Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma
Abstract Background Clear cell renal cell carcinoma (ccRCC) is the most common form of adult kidney cancer. Ubiquitin-specific protease (USP)44 has been reported to be involved in various cancers. We investigated the function, role and molecular mechanism of USP44 in ccRCC. Methods Data obtained fro...
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doaj-77b9cc41a883477e9529c8c6c2cca08e2020-11-25T02:57:58ZengBMCBMC Cancer1471-24072020-03-0120111210.1186/s12885-020-6713-yUbiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinomaJiangqiao Zhou0Tianyu Wang1Tao Qiu2Zhongbao Chen3Xiaoxiong Ma4Long Zhang5Jilin Zou6Department of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan UniversityDepartment of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan UniversityDepartment of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan UniversityDepartment of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan UniversityDepartment of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan UniversityDepartment of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan UniversityDepartment of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan UniversityAbstract Background Clear cell renal cell carcinoma (ccRCC) is the most common form of adult kidney cancer. Ubiquitin-specific protease (USP)44 has been reported to be involved in various cancers. We investigated the function, role and molecular mechanism of USP44 in ccRCC. Methods Data obtained from the Cancer Genome Atlas Data Portal and Gene Expression Omnibus database were analyzed to uncover the clinical relevance of USP44 expression and tumor development. USP44 function in the proliferation and migration of tumor cells was assessed by cellular and molecular analyses using ccRCC lines (786-O cells and Caki-1 cells). Results USP44 showed low expression in ccRCC cancer tissues compared with that in normal tissue. USP44 expression was negatively correlated with tumor stage, tumor grade, and patient survival. USP44 overexpression inhibited the proliferation and migration of 786-O cells and Caki-1 cells significantly. USP44 overexpression also prohibited cell proliferation by upregulating expression of P21, downregulating cyclin-D1 expression, and inhibiting cell migration by downregulating expression of matrix metalloproteinase (MMP)2 and MMP9. USP44 knockdown enhanced the proliferation and migration of 786-O cells and Caki-1 cells. USP44 function in inhibiting the proliferation and migration of 786-O cells and Caki-1 cells was associated with phosphorylation of Jun N-terminal kinase (JNK). Conclusion USP44 may be a marker in predicting ccRCC progression. Inhibition by USP44 of the proliferation and migration of 786-O cells and Caki-1 cells is dependent upon the JNK pathway.http://link.springer.com/article/10.1186/s12885-020-6713-yUSP44Clear cell renal cell carcinomaProliferationJNK |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiangqiao Zhou Tianyu Wang Tao Qiu Zhongbao Chen Xiaoxiong Ma Long Zhang Jilin Zou |
spellingShingle |
Jiangqiao Zhou Tianyu Wang Tao Qiu Zhongbao Chen Xiaoxiong Ma Long Zhang Jilin Zou Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma BMC Cancer USP44 Clear cell renal cell carcinoma Proliferation JNK |
author_facet |
Jiangqiao Zhou Tianyu Wang Tao Qiu Zhongbao Chen Xiaoxiong Ma Long Zhang Jilin Zou |
author_sort |
Jiangqiao Zhou |
title |
Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma |
title_short |
Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma |
title_full |
Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma |
title_fullStr |
Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma |
title_full_unstemmed |
Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma |
title_sort |
ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of jnk pathway in clear cell renal cell carcinoma |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2020-03-01 |
description |
Abstract Background Clear cell renal cell carcinoma (ccRCC) is the most common form of adult kidney cancer. Ubiquitin-specific protease (USP)44 has been reported to be involved in various cancers. We investigated the function, role and molecular mechanism of USP44 in ccRCC. Methods Data obtained from the Cancer Genome Atlas Data Portal and Gene Expression Omnibus database were analyzed to uncover the clinical relevance of USP44 expression and tumor development. USP44 function in the proliferation and migration of tumor cells was assessed by cellular and molecular analyses using ccRCC lines (786-O cells and Caki-1 cells). Results USP44 showed low expression in ccRCC cancer tissues compared with that in normal tissue. USP44 expression was negatively correlated with tumor stage, tumor grade, and patient survival. USP44 overexpression inhibited the proliferation and migration of 786-O cells and Caki-1 cells significantly. USP44 overexpression also prohibited cell proliferation by upregulating expression of P21, downregulating cyclin-D1 expression, and inhibiting cell migration by downregulating expression of matrix metalloproteinase (MMP)2 and MMP9. USP44 knockdown enhanced the proliferation and migration of 786-O cells and Caki-1 cells. USP44 function in inhibiting the proliferation and migration of 786-O cells and Caki-1 cells was associated with phosphorylation of Jun N-terminal kinase (JNK). Conclusion USP44 may be a marker in predicting ccRCC progression. Inhibition by USP44 of the proliferation and migration of 786-O cells and Caki-1 cells is dependent upon the JNK pathway. |
topic |
USP44 Clear cell renal cell carcinoma Proliferation JNK |
url |
http://link.springer.com/article/10.1186/s12885-020-6713-y |
work_keys_str_mv |
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