The Transcription Unit Architecture of Streptomyces lividans TK24
Streptomyces lividans is an attractive host for production of heterologous proteins and secondary metabolites of other Streptomyces species. To fully harness the industrial potential of S. lividans, understanding its metabolism and genetic regulatory elements is essential. This study aimed to determ...
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doaj-77c4e108e7b3465c9ff56ff2ce529e392020-11-24T21:36:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-09-011010.3389/fmicb.2019.02074467303The Transcription Unit Architecture of Streptomyces lividans TK24Yongjae Lee0Namil Lee1Yujin Jeong2Soonkyu Hwang3Woori Kim4Suhyung Cho5Bernhard O. Palsson6Bernhard O. Palsson7Bernhard O. Palsson8Byung-Kwan Cho9Byung-Kwan Cho10Systems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaSystems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaSystems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaSystems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaSystems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaSystems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaSystems Biology Research Group, Department of Bioengineering, University of California, San Diego, San Diego, CA, United StatesDepartment of Pediatrics, University of California, San Diego,San Diego, CA, United StatesNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, DenmarkSystems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaIntelligent Synthetic Biology Center, Daejeon, South KoreaStreptomyces lividans is an attractive host for production of heterologous proteins and secondary metabolites of other Streptomyces species. To fully harness the industrial potential of S. lividans, understanding its metabolism and genetic regulatory elements is essential. This study aimed to determine its transcription unit (TU) architecture and elucidate its diverse regulatory elements, including promoters, ribosome binding sites, 5′-untranslated regions, and transcription terminators. Total 1,978 transcription start sites and 1,640 transcript 3′-end positions were identified, which were integrated to determine 1,300 TUs, consistent with transcriptomic profiles. The conserved promoter sequences were found as 5′-TANNNT and 5′-TGAC, representing the −10 and −35 elements, respectively. Analysis of transcript 3′-end positions revealed the presence of distinctive terminator sequences and the RNA stem structure responsible for the determination of the 3′-boundary of a transcript. Functionally related genes are likely to be regulated simultaneously by using similar promoters and being transcribed as a poly-cistronic TU. Poly-cistronic TUs were further processed or alternatively transcribed into multiple TUs to fine-regulate individual genes in response to environmental conditions. The TU information and regulatory elements identified will serve as invaluable resources for understanding the complex regulatory mechanisms of S. lividans and to elevate its industrial potential.https://www.frontiersin.org/article/10.3389/fmicb.2019.02074/fullStreptomycestranscriptiontranscription start sitetranscription terminationtranscription unit |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yongjae Lee Namil Lee Yujin Jeong Soonkyu Hwang Woori Kim Suhyung Cho Bernhard O. Palsson Bernhard O. Palsson Bernhard O. Palsson Byung-Kwan Cho Byung-Kwan Cho |
spellingShingle |
Yongjae Lee Namil Lee Yujin Jeong Soonkyu Hwang Woori Kim Suhyung Cho Bernhard O. Palsson Bernhard O. Palsson Bernhard O. Palsson Byung-Kwan Cho Byung-Kwan Cho The Transcription Unit Architecture of Streptomyces lividans TK24 Frontiers in Microbiology Streptomyces transcription transcription start site transcription termination transcription unit |
author_facet |
Yongjae Lee Namil Lee Yujin Jeong Soonkyu Hwang Woori Kim Suhyung Cho Bernhard O. Palsson Bernhard O. Palsson Bernhard O. Palsson Byung-Kwan Cho Byung-Kwan Cho |
author_sort |
Yongjae Lee |
title |
The Transcription Unit Architecture of Streptomyces lividans TK24 |
title_short |
The Transcription Unit Architecture of Streptomyces lividans TK24 |
title_full |
The Transcription Unit Architecture of Streptomyces lividans TK24 |
title_fullStr |
The Transcription Unit Architecture of Streptomyces lividans TK24 |
title_full_unstemmed |
The Transcription Unit Architecture of Streptomyces lividans TK24 |
title_sort |
transcription unit architecture of streptomyces lividans tk24 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-09-01 |
description |
Streptomyces lividans is an attractive host for production of heterologous proteins and secondary metabolites of other Streptomyces species. To fully harness the industrial potential of S. lividans, understanding its metabolism and genetic regulatory elements is essential. This study aimed to determine its transcription unit (TU) architecture and elucidate its diverse regulatory elements, including promoters, ribosome binding sites, 5′-untranslated regions, and transcription terminators. Total 1,978 transcription start sites and 1,640 transcript 3′-end positions were identified, which were integrated to determine 1,300 TUs, consistent with transcriptomic profiles. The conserved promoter sequences were found as 5′-TANNNT and 5′-TGAC, representing the −10 and −35 elements, respectively. Analysis of transcript 3′-end positions revealed the presence of distinctive terminator sequences and the RNA stem structure responsible for the determination of the 3′-boundary of a transcript. Functionally related genes are likely to be regulated simultaneously by using similar promoters and being transcribed as a poly-cistronic TU. Poly-cistronic TUs were further processed or alternatively transcribed into multiple TUs to fine-regulate individual genes in response to environmental conditions. The TU information and regulatory elements identified will serve as invaluable resources for understanding the complex regulatory mechanisms of S. lividans and to elevate its industrial potential. |
topic |
Streptomyces transcription transcription start site transcription termination transcription unit |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02074/full |
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