CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis
Objective: To examine the effect of connective tissue growth factor (CTGF)-mediated ERK signaling pathway on the inflammatory response and intestinal flora in ulcerative colitis (UC). Methods: CTGF expression was determined through immunohistochemistry in UC and colon polyp patients. Dextran sulfate...
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doaj-77ca44e954cf45c7a335e1d75c8e33532021-05-20T07:35:51ZengElsevierBiomedicine & Pharmacotherapy0753-33222019-03-0111114291437CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitisZhen-Mei Song0Fang Liu1Yan-Ming Chen2Yi-Jing Liu3Xiao-Di Wang4Shi-Yu Du5Department of Gastroenterology, China-Japan Friendship Hospital, Beijing, 100029, PR ChinaDepartment of Gastroenterology, China-Japan Friendship Hospital, Beijing, 100029, PR ChinaDepartment of Gastroenterology, China-Japan Friendship Hospital, Beijing, 100029, PR ChinaDepartment of Gastroenterology, China-Japan Friendship Hospital, Beijing, 100029, PR ChinaDepartment of Gastroenterology, China-Japan Friendship Hospital, Beijing, 100029, PR ChinaCorresponding author at: Department of Gastroenterology, China-Japan Friendship Hospital, No. 2, Yinghua East Street, Chaoyang District, Beijing, 100029, PR China.; Department of Gastroenterology, China-Japan Friendship Hospital, Beijing, 100029, PR ChinaObjective: To examine the effect of connective tissue growth factor (CTGF)-mediated ERK signaling pathway on the inflammatory response and intestinal flora in ulcerative colitis (UC). Methods: CTGF expression was determined through immunohistochemistry in UC and colon polyp patients. Dextran sulfate sodium (DSS) was used to construct UC models. Wild-type (WT) and CTGF-deficient (CTGF−/−) mice were randomly divided into WT/CTGF−/− + saline, WT/CTGF−/− + DSS, and WT/CTGF−/− + DSS + U0126 (ERK pathway inhibitor) groups. HE staining was conducted to observe the pathological changes in intestinal mucosa. The quantity of intestinal flora was tested in the feces. ELISA, qRT-PCR, and Western blotting were used to detect related-molecules expressions. Results: CTGF was up-regulated in the intestinal mucosa of UC patients in relation to the severity and grade. Moreover, UC patients showed enhanced the expressions of p-ERK/ERK and pro-inflammatory factors (IL-1β, IL-6, TNF-α, MPO), increased the quantity of Bacteriodes fragilis (B. fragilis) and Escherichia coli (E. coli), and decreased Bifidobacterium and Lactobacillus. CTGF and pERK/ERK expressions were increased in DSS-induced WT mice, but the pERK expression was lower in CTGF−/− + DSS group than that in the WT + DSS group. U0126 decreased the expressions of pro-inflammatory factors and improved the intestinal flora in WT mice induced with DSS. No significant differences were found in the above indexes between CTGF−/− + DSS group and WT + DSS + U0126 group. Conclusion: Inhibiting CTGF could improve inflammatory response and intestinal flora to partially reverse DSS-induced UC via blocking ERK signaling pathway.http://www.sciencedirect.com/science/article/pii/S0753332218358499Ulcerative colitisCTGFEKRInflammatory factorsIntestinal flora |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhen-Mei Song Fang Liu Yan-Ming Chen Yi-Jing Liu Xiao-Di Wang Shi-Yu Du |
spellingShingle |
Zhen-Mei Song Fang Liu Yan-Ming Chen Yi-Jing Liu Xiao-Di Wang Shi-Yu Du CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis Biomedicine & Pharmacotherapy Ulcerative colitis CTGF EKR Inflammatory factors Intestinal flora |
author_facet |
Zhen-Mei Song Fang Liu Yan-Ming Chen Yi-Jing Liu Xiao-Di Wang Shi-Yu Du |
author_sort |
Zhen-Mei Song |
title |
CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis |
title_short |
CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis |
title_full |
CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis |
title_fullStr |
CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis |
title_full_unstemmed |
CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis |
title_sort |
ctgf-mediated erk signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2019-03-01 |
description |
Objective: To examine the effect of connective tissue growth factor (CTGF)-mediated ERK signaling pathway on the inflammatory response and intestinal flora in ulcerative colitis (UC). Methods: CTGF expression was determined through immunohistochemistry in UC and colon polyp patients. Dextran sulfate sodium (DSS) was used to construct UC models. Wild-type (WT) and CTGF-deficient (CTGF−/−) mice were randomly divided into WT/CTGF−/− + saline, WT/CTGF−/− + DSS, and WT/CTGF−/− + DSS + U0126 (ERK pathway inhibitor) groups. HE staining was conducted to observe the pathological changes in intestinal mucosa. The quantity of intestinal flora was tested in the feces. ELISA, qRT-PCR, and Western blotting were used to detect related-molecules expressions. Results: CTGF was up-regulated in the intestinal mucosa of UC patients in relation to the severity and grade. Moreover, UC patients showed enhanced the expressions of p-ERK/ERK and pro-inflammatory factors (IL-1β, IL-6, TNF-α, MPO), increased the quantity of Bacteriodes fragilis (B. fragilis) and Escherichia coli (E. coli), and decreased Bifidobacterium and Lactobacillus. CTGF and pERK/ERK expressions were increased in DSS-induced WT mice, but the pERK expression was lower in CTGF−/− + DSS group than that in the WT + DSS group. U0126 decreased the expressions of pro-inflammatory factors and improved the intestinal flora in WT mice induced with DSS. No significant differences were found in the above indexes between CTGF−/− + DSS group and WT + DSS + U0126 group. Conclusion: Inhibiting CTGF could improve inflammatory response and intestinal flora to partially reverse DSS-induced UC via blocking ERK signaling pathway. |
topic |
Ulcerative colitis CTGF EKR Inflammatory factors Intestinal flora |
url |
http://www.sciencedirect.com/science/article/pii/S0753332218358499 |
work_keys_str_mv |
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