Effects of N Fertilization During Cultivation and Lactobacillus plantarum Inoculation at Ensiling on Chemical Composition and Bacterial Community of Mulberry Silage
As unconventional forage source, mulberry (Morus alba L.) has been cultivated to alleviate animal feed shortages. This study aimed to investigate the effects of N fertilization during cultivation and Lactobacillus plantarum inoculation at ensiling on the chemical composition and bacterial community...
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doaj-77cdb093b6d34a59b774504221bd76ae2021-10-07T06:49:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-10-011210.3389/fmicb.2021.735767735767Effects of N Fertilization During Cultivation and Lactobacillus plantarum Inoculation at Ensiling on Chemical Composition and Bacterial Community of Mulberry SilagePing Li0Ping Li1Minghong You2Zhouhe Du3Yongxiang Lu4Chunyan Zuo5Man Zhao6Honglin Wang7Xu Yan8Chao Chen9College of Animal Science, Guizhou University, Guiyang, ChinaGrass and Forage Research Institute, Sichuan Academy of Grassland Sciences, Chengdu, ChinaGrass and Forage Research Institute, Sichuan Academy of Grassland Sciences, Chengdu, ChinaSericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong, ChinaGrass and Forage Research Institute, Sichuan Academy of Grassland Sciences, Chengdu, ChinaSericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong, ChinaGrass and Forage Research Institute, Sichuan Academy of Grassland Sciences, Chengdu, ChinaSericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong, ChinaSericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaAs unconventional forage source, mulberry (Morus alba L.) has been cultivated to alleviate animal feed shortages. This study aimed to investigate the effects of N fertilization during cultivation and Lactobacillus plantarum inoculation at ensiling on the chemical composition and bacterial community of mulberry silage. Mulberry was separately cultivated under two N fertilization rates (N1, 390 kg/ha/year; N2, 485 kg/ha/year) in 2016–2019, harvested on 30 April (the first-cut) and 15 June (the second-cut) in 2019, and then chopped for producing small bag silage. The silage was treated without (control) or with L. plantarum (LP, a recommended application rate of 105 cfu/g on fresh matter basis). After storage of 60 days in dark room at ambient temperature, silage was sampled for analysis of chemical and microbial compositions. Higher (P < 0.05) final pH value and acetic acid content and lower (P < 0.05) lactic acid content were found in silage of mulberry under N2 fertilization, resulting in more dry matter loss than that under N1 fertilization. Compared with control, inoculation of LP at ensiling increased (P < 0.05) lactic acid content and decreased (P < 0.05) final pH value, acetic acid and propionic acid contents of silage, by advancing the dominance of Lactobacillus and reducing the abundance of Enterococcus and Enterobacter. In particular, inoculation of LP at ensiling decreased (P < 0.05) dry matter loss and butyric acid content of first-cut silage. In conclusion, inoculation of LP at ensiling could reduce the undesirable effects from high N fertilization rate during cultivation on silage quality of mulberry harvested at different growing seasons.https://www.frontiersin.org/articles/10.3389/fmicb.2021.735767/fullmulberryN fertilizationsilagehigh throughput sequencingbacterial community |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ping Li Ping Li Minghong You Zhouhe Du Yongxiang Lu Chunyan Zuo Man Zhao Honglin Wang Xu Yan Chao Chen |
spellingShingle |
Ping Li Ping Li Minghong You Zhouhe Du Yongxiang Lu Chunyan Zuo Man Zhao Honglin Wang Xu Yan Chao Chen Effects of N Fertilization During Cultivation and Lactobacillus plantarum Inoculation at Ensiling on Chemical Composition and Bacterial Community of Mulberry Silage Frontiers in Microbiology mulberry N fertilization silage high throughput sequencing bacterial community |
author_facet |
Ping Li Ping Li Minghong You Zhouhe Du Yongxiang Lu Chunyan Zuo Man Zhao Honglin Wang Xu Yan Chao Chen |
author_sort |
Ping Li |
title |
Effects of N Fertilization During Cultivation and Lactobacillus plantarum Inoculation at Ensiling on Chemical Composition and Bacterial Community of Mulberry Silage |
title_short |
Effects of N Fertilization During Cultivation and Lactobacillus plantarum Inoculation at Ensiling on Chemical Composition and Bacterial Community of Mulberry Silage |
title_full |
Effects of N Fertilization During Cultivation and Lactobacillus plantarum Inoculation at Ensiling on Chemical Composition and Bacterial Community of Mulberry Silage |
title_fullStr |
Effects of N Fertilization During Cultivation and Lactobacillus plantarum Inoculation at Ensiling on Chemical Composition and Bacterial Community of Mulberry Silage |
title_full_unstemmed |
Effects of N Fertilization During Cultivation and Lactobacillus plantarum Inoculation at Ensiling on Chemical Composition and Bacterial Community of Mulberry Silage |
title_sort |
effects of n fertilization during cultivation and lactobacillus plantarum inoculation at ensiling on chemical composition and bacterial community of mulberry silage |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-10-01 |
description |
As unconventional forage source, mulberry (Morus alba L.) has been cultivated to alleviate animal feed shortages. This study aimed to investigate the effects of N fertilization during cultivation and Lactobacillus plantarum inoculation at ensiling on the chemical composition and bacterial community of mulberry silage. Mulberry was separately cultivated under two N fertilization rates (N1, 390 kg/ha/year; N2, 485 kg/ha/year) in 2016–2019, harvested on 30 April (the first-cut) and 15 June (the second-cut) in 2019, and then chopped for producing small bag silage. The silage was treated without (control) or with L. plantarum (LP, a recommended application rate of 105 cfu/g on fresh matter basis). After storage of 60 days in dark room at ambient temperature, silage was sampled for analysis of chemical and microbial compositions. Higher (P < 0.05) final pH value and acetic acid content and lower (P < 0.05) lactic acid content were found in silage of mulberry under N2 fertilization, resulting in more dry matter loss than that under N1 fertilization. Compared with control, inoculation of LP at ensiling increased (P < 0.05) lactic acid content and decreased (P < 0.05) final pH value, acetic acid and propionic acid contents of silage, by advancing the dominance of Lactobacillus and reducing the abundance of Enterococcus and Enterobacter. In particular, inoculation of LP at ensiling decreased (P < 0.05) dry matter loss and butyric acid content of first-cut silage. In conclusion, inoculation of LP at ensiling could reduce the undesirable effects from high N fertilization rate during cultivation on silage quality of mulberry harvested at different growing seasons. |
topic |
mulberry N fertilization silage high throughput sequencing bacterial community |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.735767/full |
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