Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico Gómez
The Acinetobacter calcoaceticus-baumannii (Acb) complex is regarded as a group of phenotypically indistinguishable opportunistic pathogens responsible for mainly causing hospital-acquired pneumonia and bacteremia. The aim of this study was to determine the frequency of isolation of the species that...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-10-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.576673/full |
id |
doaj-77d8c11e66e64ccab89da92ebbd1353e |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jetsi Mancilla-Rojano Jetsi Mancilla-Rojano Sara A. Ochoa Juan Pablo Reyes-Grajeda Víctor Flores Oscar Medina-Contreras Karina Espinosa-Mazariego Israel Parra-Ortega Daniela De La Rosa-Zamboni María del Carmen Castellanos-Cruz José Arellano-Galindo Miguel A. Cevallos Rigoberto Hernández-Castro Juan Xicohtencatl-Cortes Ariadnna Cruz-Córdova |
spellingShingle |
Jetsi Mancilla-Rojano Jetsi Mancilla-Rojano Sara A. Ochoa Juan Pablo Reyes-Grajeda Víctor Flores Oscar Medina-Contreras Karina Espinosa-Mazariego Israel Parra-Ortega Daniela De La Rosa-Zamboni María del Carmen Castellanos-Cruz José Arellano-Galindo Miguel A. Cevallos Rigoberto Hernández-Castro Juan Xicohtencatl-Cortes Ariadnna Cruz-Córdova Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico Gómez Frontiers in Microbiology Acinetobacter baumannii Acinetobacter calcoaceticus-Acinetobacter baumannii complex intensive care unit resistance molecular typing |
author_facet |
Jetsi Mancilla-Rojano Jetsi Mancilla-Rojano Sara A. Ochoa Juan Pablo Reyes-Grajeda Víctor Flores Oscar Medina-Contreras Karina Espinosa-Mazariego Israel Parra-Ortega Daniela De La Rosa-Zamboni María del Carmen Castellanos-Cruz José Arellano-Galindo Miguel A. Cevallos Rigoberto Hernández-Castro Juan Xicohtencatl-Cortes Ariadnna Cruz-Córdova |
author_sort |
Jetsi Mancilla-Rojano |
title |
Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico Gómez |
title_short |
Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico Gómez |
title_full |
Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico Gómez |
title_fullStr |
Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico Gómez |
title_full_unstemmed |
Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico Gómez |
title_sort |
molecular epidemiology of acinetobacter calcoaceticus-acinetobacter baumannii complex isolated from children at the hospital infantil de méxico federico gómez |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-10-01 |
description |
The Acinetobacter calcoaceticus-baumannii (Acb) complex is regarded as a group of phenotypically indistinguishable opportunistic pathogens responsible for mainly causing hospital-acquired pneumonia and bacteremia. The aim of this study was to determine the frequency of isolation of the species that constitute the Acb complex, as well as their susceptibility to antibiotics, and their distribution at the Hospital Infantil de Mexico Federico Gomez (HIMFG). A total of 88 strains previously identified by Vitek 2®, 40 as Acinetobacter baumannii and 48 as Acb complex were isolated from 52 children from 07, January 2015 to 28, September 2017. A. baumannii accounted for 89.77% (79/88) of the strains; Acinetobacter pittii, 6.82% (6/88); and Acinetobacter nosocomialis, 3.40% (3/88). Most strains were recovered mainly from patients in the intensive care unit (ICU) and emergency wards. Blood cultures (BC) provided 44.32% (39/88) of strains. The 13.63% (12/88) of strains were associated with primary bacteremia, 3.4% (3/88) with secondary bacteremia, and 2.3% (2/88) with pneumonia. In addition, 44.32% (39/88) were multidrug-resistant (MDR) strains and, 11.36% (10/88) were extensively drug-resistant (XDR). All strains amplified the blaOXA-51 gene; 51.13% (45/88), the blaOXA-23 gene; 4.54% (4/88), the blaOXA-24 gene; and 2.27% (2/88), the blaOXA-58 gene. Plasmid profiles showed that the strains had 1–6 plasmids. The strains were distributed in 52 pulsotypes, and 24 showed identical restriction patterns, with a correlation coefficient of 1.0. Notably, some strains with the same pulsotype were isolated from different patients, wards, or years, suggesting the persistence of more than one clone. Twenty-seven sequence types (STs) were determined for the strains based on a Pasteur multilocus sequence typing (MLST) scheme using massive sequencing; the most prevalent was ST 156 (27.27%, 24/88). The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas I-Fb system provided amplification in A. baumannii and A. pittii strains (22.73%, 20/88). This study identified an increased number of MDR strains and the relationship among strains through molecular typing. The data suggest that more than one strain could be causing an infection in some patient. The implementation of molecular epidemiology allowed the characterization of a set of strains and identification of different attributes associated with its distribution in a specific environment. |
topic |
Acinetobacter baumannii Acinetobacter calcoaceticus-Acinetobacter baumannii complex intensive care unit resistance molecular typing |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.576673/full |
work_keys_str_mv |
AT jetsimancillarojano molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT jetsimancillarojano molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT saraaochoa molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT juanpabloreyesgrajeda molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT victorflores molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT oscarmedinacontreras molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT karinaespinosamazariego molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT israelparraortega molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT danieladelarosazamboni molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT mariadelcarmencastellanoscruz molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT josearellanogalindo molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT miguelacevallos molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT rigobertohernandezcastro molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT juanxicohtencatlcortes molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez AT ariadnnacruzcordova molecularepidemiologyofacinetobactercalcoaceticusacinetobacterbaumanniicomplexisolatedfromchildrenatthehospitalinfantildemexicofedericogomez |
_version_ |
1724463386386759680 |
spelling |
doaj-77d8c11e66e64ccab89da92ebbd1353e2020-11-25T03:56:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-10-011110.3389/fmicb.2020.576673576673Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico GómezJetsi Mancilla-Rojano0Jetsi Mancilla-Rojano1Sara A. Ochoa2Juan Pablo Reyes-Grajeda3Víctor Flores4Oscar Medina-Contreras5Karina Espinosa-Mazariego6Israel Parra-Ortega7Daniela De La Rosa-Zamboni8María del Carmen Castellanos-Cruz9José Arellano-Galindo10Miguel A. Cevallos11Rigoberto Hernández-Castro12Juan Xicohtencatl-Cortes13Ariadnna Cruz-Córdova14Laboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, MexicoFacultad de Medicina, Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, CDMX, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, MexicoSubdirección de Desarrollo de Aplicaciones Clínicas, Instituto Nacional de Medicina Genómica, Ciudad de México, MexicoUnidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Irapuato, MexicoUnidad de Investigación Epidemiológica en Endocrinología y Nutrición, Hospital Infantil de México Federico Gómez, CDMX, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, MexicoDepartamento de Laboratorio Clínico, Laboratorio Central, Hospital Infantil de México Federico Gómez, CDMX, MexicoDepartamento de Epidemiología Hospitalaria, Hospital Infantil de México Federico Gómez, CDMX, MexicoDepartamento de Laboratorio Clínico, Laboratorio Central, Hospital Infantil de México Federico Gómez, CDMX, MexicoUnidad de investigación en Enfermedades Infecciosas, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, MexicoCentro de Ciencias Genómicas, Programa de Genómica Evolutiva, Universidad Nacional Autónoma de México, Cuernavaca, Mexico0Departamento de Ecología de Agentes Patógenos, Hospital General Dr. Manuel Gea González, CDMX, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, MexicoThe Acinetobacter calcoaceticus-baumannii (Acb) complex is regarded as a group of phenotypically indistinguishable opportunistic pathogens responsible for mainly causing hospital-acquired pneumonia and bacteremia. The aim of this study was to determine the frequency of isolation of the species that constitute the Acb complex, as well as their susceptibility to antibiotics, and their distribution at the Hospital Infantil de Mexico Federico Gomez (HIMFG). A total of 88 strains previously identified by Vitek 2®, 40 as Acinetobacter baumannii and 48 as Acb complex were isolated from 52 children from 07, January 2015 to 28, September 2017. A. baumannii accounted for 89.77% (79/88) of the strains; Acinetobacter pittii, 6.82% (6/88); and Acinetobacter nosocomialis, 3.40% (3/88). Most strains were recovered mainly from patients in the intensive care unit (ICU) and emergency wards. Blood cultures (BC) provided 44.32% (39/88) of strains. The 13.63% (12/88) of strains were associated with primary bacteremia, 3.4% (3/88) with secondary bacteremia, and 2.3% (2/88) with pneumonia. In addition, 44.32% (39/88) were multidrug-resistant (MDR) strains and, 11.36% (10/88) were extensively drug-resistant (XDR). All strains amplified the blaOXA-51 gene; 51.13% (45/88), the blaOXA-23 gene; 4.54% (4/88), the blaOXA-24 gene; and 2.27% (2/88), the blaOXA-58 gene. Plasmid profiles showed that the strains had 1–6 plasmids. The strains were distributed in 52 pulsotypes, and 24 showed identical restriction patterns, with a correlation coefficient of 1.0. Notably, some strains with the same pulsotype were isolated from different patients, wards, or years, suggesting the persistence of more than one clone. Twenty-seven sequence types (STs) were determined for the strains based on a Pasteur multilocus sequence typing (MLST) scheme using massive sequencing; the most prevalent was ST 156 (27.27%, 24/88). The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas I-Fb system provided amplification in A. baumannii and A. pittii strains (22.73%, 20/88). This study identified an increased number of MDR strains and the relationship among strains through molecular typing. The data suggest that more than one strain could be causing an infection in some patient. The implementation of molecular epidemiology allowed the characterization of a set of strains and identification of different attributes associated with its distribution in a specific environment.https://www.frontiersin.org/article/10.3389/fmicb.2020.576673/fullAcinetobacter baumanniiAcinetobacter calcoaceticus-Acinetobacter baumannii complexintensive care unitresistancemolecular typing |