Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs
Nipah virus (NiV) is an emergent pathogen capable of causing acute respiratory illness and fatal encephalitis in pigs and humans. A high fatality rate and broad host tropism makes NiV a serious public and animal health concern. There is therefore an urgent need for a NiV vaccines to protect animals...
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doaj-77d9cdd0f6ef4f9aa01a5e119bae59532020-11-25T02:28:23ZengMDPI AGVaccines2076-393X2020-03-018111510.3390/vaccines8010115vaccines8010115Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in PigsMiriam Pedrera0Francesca Macchi1Rebecca K. McLean2Valentina Franceschi3Nazia Thakur4Luca Russo5Lobna Medfai6Shawn Todd7Elma Z. Tchilian8Jean-Christophe Audonnet9Keith Chappell10Ariel Isaacs11Daniel Watterson12Paul R. Young13Glenn A. Marsh14Dalan Bailey15Simon P. Graham16Gaetano Donofrio17The Pirbright Institute, Ash Road, Pirbright GU24 0NF, UKDepartment of Medical-Veterinary Science, University of Parma, 43126 Parma, ItalyThe Pirbright Institute, Ash Road, Pirbright GU24 0NF, UKDepartment of Medical-Veterinary Science, University of Parma, 43126 Parma, ItalyThe Pirbright Institute, Ash Road, Pirbright GU24 0NF, UKDepartment of Medical-Veterinary Science, University of Parma, 43126 Parma, ItalyThe Pirbright Institute, Ash Road, Pirbright GU24 0NF, UKCSIRO Health and Biosecurity, Australian Animal Health Laboratory, Geelong, VIC 3219, AustraliaThe Pirbright Institute, Ash Road, Pirbright GU24 0NF, UKBoehringer Ingelheim Animal Health, Bâtiment 700 R&D, 813 Cours du 3ème Millénaire, 69800 Saint Priest, FranceAustralian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, QLD 4072, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, QLD 4072, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, QLD 4072, AustraliaAustralian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, QLD 4072, AustraliaCSIRO Health and Biosecurity, Australian Animal Health Laboratory, Geelong, VIC 3219, AustraliaThe Pirbright Institute, Ash Road, Pirbright GU24 0NF, UKThe Pirbright Institute, Ash Road, Pirbright GU24 0NF, UKDepartment of Medical-Veterinary Science, University of Parma, 43126 Parma, ItalyNipah virus (NiV) is an emergent pathogen capable of causing acute respiratory illness and fatal encephalitis in pigs and humans. A high fatality rate and broad host tropism makes NiV a serious public and animal health concern. There is therefore an urgent need for a NiV vaccines to protect animals and humans. In this study we investigated the immunogenicity of bovine herpesvirus (BoHV-4) vectors expressing either NiV attachment (G) or fusion (F) glycoproteins, BoHV-4-A-CMV-NiV-GΔTK or BoHV-4-A-CMV-NiV-FΔTK, respectively in pigs. The vaccines were benchmarked against a canarypox (ALVAC) vector expressing NiV G, previously demonstrated to induce protective immunity in pigs. Both BoHV-4 vectors induced robust antigen-specific antibody responses. BoHV-4-A-CMV-NiV-GΔTK stimulated NiV-neutralizing antibody titers comparable to ALVAC NiV G and greater than those induced by BoHV-4-A-CMV-NiV-FΔTK. In contrast, only BoHV-4-A-CMV-NiV-FΔTK immunized pigs had antibodies capable of significantly neutralizing NiV G and F-mediated cell fusion. All three vectored vaccines evoked antigen-specific CD4 and CD8 T cell responses, which were particularly strong in BoHV-4-A-CMV-NiV-GΔTK immunized pigs and to a lesser extent BoHV-4-A-CMV-NiV-FΔTK. These findings emphasize the potential of BoHV-4 vectors for inducing antibody and cell-mediated immunity in pigs and provide a solid basis for the further evaluation of these vectored NiV vaccine candidates.https://www.mdpi.com/2076-393X/8/1/115nipah virusbovine herpes virus 4vaccinepigimmunogenicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miriam Pedrera Francesca Macchi Rebecca K. McLean Valentina Franceschi Nazia Thakur Luca Russo Lobna Medfai Shawn Todd Elma Z. Tchilian Jean-Christophe Audonnet Keith Chappell Ariel Isaacs Daniel Watterson Paul R. Young Glenn A. Marsh Dalan Bailey Simon P. Graham Gaetano Donofrio |
spellingShingle |
Miriam Pedrera Francesca Macchi Rebecca K. McLean Valentina Franceschi Nazia Thakur Luca Russo Lobna Medfai Shawn Todd Elma Z. Tchilian Jean-Christophe Audonnet Keith Chappell Ariel Isaacs Daniel Watterson Paul R. Young Glenn A. Marsh Dalan Bailey Simon P. Graham Gaetano Donofrio Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs Vaccines nipah virus bovine herpes virus 4 vaccine pig immunogenicity |
author_facet |
Miriam Pedrera Francesca Macchi Rebecca K. McLean Valentina Franceschi Nazia Thakur Luca Russo Lobna Medfai Shawn Todd Elma Z. Tchilian Jean-Christophe Audonnet Keith Chappell Ariel Isaacs Daniel Watterson Paul R. Young Glenn A. Marsh Dalan Bailey Simon P. Graham Gaetano Donofrio |
author_sort |
Miriam Pedrera |
title |
Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs |
title_short |
Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs |
title_full |
Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs |
title_fullStr |
Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs |
title_full_unstemmed |
Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs |
title_sort |
bovine herpesvirus-4-vectored delivery of nipah virus glycoproteins enhances t cell immunogenicity in pigs |
publisher |
MDPI AG |
series |
Vaccines |
issn |
2076-393X |
publishDate |
2020-03-01 |
description |
Nipah virus (NiV) is an emergent pathogen capable of causing acute respiratory illness and fatal encephalitis in pigs and humans. A high fatality rate and broad host tropism makes NiV a serious public and animal health concern. There is therefore an urgent need for a NiV vaccines to protect animals and humans. In this study we investigated the immunogenicity of bovine herpesvirus (BoHV-4) vectors expressing either NiV attachment (G) or fusion (F) glycoproteins, BoHV-4-A-CMV-NiV-GΔTK or BoHV-4-A-CMV-NiV-FΔTK, respectively in pigs. The vaccines were benchmarked against a canarypox (ALVAC) vector expressing NiV G, previously demonstrated to induce protective immunity in pigs. Both BoHV-4 vectors induced robust antigen-specific antibody responses. BoHV-4-A-CMV-NiV-GΔTK stimulated NiV-neutralizing antibody titers comparable to ALVAC NiV G and greater than those induced by BoHV-4-A-CMV-NiV-FΔTK. In contrast, only BoHV-4-A-CMV-NiV-FΔTK immunized pigs had antibodies capable of significantly neutralizing NiV G and F-mediated cell fusion. All three vectored vaccines evoked antigen-specific CD4 and CD8 T cell responses, which were particularly strong in BoHV-4-A-CMV-NiV-GΔTK immunized pigs and to a lesser extent BoHV-4-A-CMV-NiV-FΔTK. These findings emphasize the potential of BoHV-4 vectors for inducing antibody and cell-mediated immunity in pigs and provide a solid basis for the further evaluation of these vectored NiV vaccine candidates. |
topic |
nipah virus bovine herpes virus 4 vaccine pig immunogenicity |
url |
https://www.mdpi.com/2076-393X/8/1/115 |
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