Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China
A total of 154 non-duplicate Salmonella isolates were recovered from 1,105 rectal swabs collected from three large-scale chicken farms (78/325, 24.0%), three large-scale duck farms (56/600, 9.3%) and three large-scale pig farms (20/180, 11.1%) between April and July 2016. Seven serotypes were identi...
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doaj-77e9003b31334ec88edbcad148fbfc562020-11-24T23:59:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-07-01810.3389/fmicb.2017.01300270759Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, ChinaXiaonan Zhao0Jie Yang1Baozhen Zhang2Shuhong Sun3Weishan Chang4College of Animal Science and Technology, Shandong Agricultural UniversityTai'an, ChinaCollege of Animal Science and Technology, Shandong Agricultural UniversityTai'an, ChinaLaboratory for Reproduction Health Institute of Biomedicine and Biotechnology Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen University TownShenzhen, ChinaCollege of Animal Science and Technology, Shandong Agricultural UniversityTai'an, ChinaCollege of Animal Science and Technology, Shandong Agricultural UniversityTai'an, ChinaA total of 154 non-duplicate Salmonella isolates were recovered from 1,105 rectal swabs collected from three large-scale chicken farms (78/325, 24.0%), three large-scale duck farms (56/600, 9.3%) and three large-scale pig farms (20/180, 11.1%) between April and July 2016. Seven serotypes were identified among the 154 isolates, with the most common serotype in chickens and ducks being Salmonella enteritidis and in pigs Salmonella typhimurium. Antimicrobial susceptibility testing revealed that high antimicrobial resistance rates were observed for tetracycline (72.0%) and ampicillin (69.4%) in all sources. Class 1 integrons were detected in 16.9% (26/154) of these isolates and contained gene cassettes aadA2, aadA1, drfA1-aadA1, drfA12-aadA2, and drfA17-aadA5. Three β-lactamase genes were detected among the 154 isolates, and most of the isolates carried blaTEM−1(55/154), followed by blaPSE−1(14/154) and blaCTX−M−55 (11/154). Three plasmid-mediated quinolone resistance genes were detected among the 154 isolates, and most of the isolates carried qnrA (113/154), followed by qnrB (99/154) and qnrS (10/154). Fifty-four isolates carried floR among the 154 isolates. Multilocus sequence typing (MLST) analysis showed that nine sequence types (STs) were identified; ST11 was the most frequent genotype in chickens and ducks, and ST19 was identified in pigs. Our findings indicated that Salmonella was widespread, and the overuse of antibiotics in animals should be reduced considerably in developing countries.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01300/fullSalmonellaantimicrobial susceptibilityclass 1 integronantimicrobial resistance geneMLST |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaonan Zhao Jie Yang Baozhen Zhang Shuhong Sun Weishan Chang |
spellingShingle |
Xiaonan Zhao Jie Yang Baozhen Zhang Shuhong Sun Weishan Chang Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China Frontiers in Microbiology Salmonella antimicrobial susceptibility class 1 integron antimicrobial resistance gene MLST |
author_facet |
Xiaonan Zhao Jie Yang Baozhen Zhang Shuhong Sun Weishan Chang |
author_sort |
Xiaonan Zhao |
title |
Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China |
title_short |
Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China |
title_full |
Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China |
title_fullStr |
Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China |
title_full_unstemmed |
Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China |
title_sort |
characterization of integrons and resistance genes in salmonella isolates from farm animals in shandong province, china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-07-01 |
description |
A total of 154 non-duplicate Salmonella isolates were recovered from 1,105 rectal swabs collected from three large-scale chicken farms (78/325, 24.0%), three large-scale duck farms (56/600, 9.3%) and three large-scale pig farms (20/180, 11.1%) between April and July 2016. Seven serotypes were identified among the 154 isolates, with the most common serotype in chickens and ducks being Salmonella enteritidis and in pigs Salmonella typhimurium. Antimicrobial susceptibility testing revealed that high antimicrobial resistance rates were observed for tetracycline (72.0%) and ampicillin (69.4%) in all sources. Class 1 integrons were detected in 16.9% (26/154) of these isolates and contained gene cassettes aadA2, aadA1, drfA1-aadA1, drfA12-aadA2, and drfA17-aadA5. Three β-lactamase genes were detected among the 154 isolates, and most of the isolates carried blaTEM−1(55/154), followed by blaPSE−1(14/154) and blaCTX−M−55 (11/154). Three plasmid-mediated quinolone resistance genes were detected among the 154 isolates, and most of the isolates carried qnrA (113/154), followed by qnrB (99/154) and qnrS (10/154). Fifty-four isolates carried floR among the 154 isolates. Multilocus sequence typing (MLST) analysis showed that nine sequence types (STs) were identified; ST11 was the most frequent genotype in chickens and ducks, and ST19 was identified in pigs. Our findings indicated that Salmonella was widespread, and the overuse of antibiotics in animals should be reduced considerably in developing countries. |
topic |
Salmonella antimicrobial susceptibility class 1 integron antimicrobial resistance gene MLST |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01300/full |
work_keys_str_mv |
AT xiaonanzhao characterizationofintegronsandresistancegenesinsalmonellaisolatesfromfarmanimalsinshandongprovincechina AT jieyang characterizationofintegronsandresistancegenesinsalmonellaisolatesfromfarmanimalsinshandongprovincechina AT baozhenzhang characterizationofintegronsandresistancegenesinsalmonellaisolatesfromfarmanimalsinshandongprovincechina AT shuhongsun characterizationofintegronsandresistancegenesinsalmonellaisolatesfromfarmanimalsinshandongprovincechina AT weishanchang characterizationofintegronsandresistancegenesinsalmonellaisolatesfromfarmanimalsinshandongprovincechina |
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