The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2
ABSTRACT The standard aqueous stem bark extract is consumed as herbal drink and used in the pharmaceutical formulations to treat patients suffering from various disease conditions in Cuba. This study was carried out to evaluate the modulatory effect of standard aqueous bark extract of M. indica on G...
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2016-03-01
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doaj-77ed0b6ef46146d399dd69643b5dacf92020-11-24T22:15:16ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902016-03-0188119720910.1590/0001-3765201620140574S0001-37652016000100197The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2BHADRAPURA LAKKAPPA DHANANJAYASUDHARSHAN SHIVALINGAIAHABSTRACT The standard aqueous stem bark extract is consumed as herbal drink and used in the pharmaceutical formulations to treat patients suffering from various disease conditions in Cuba. This study was carried out to evaluate the modulatory effect of standard aqueous bark extract of M. indica on Group IA sPLA2. M. indica extract, dose dependently inhibited the GIA sPLA2 (NN-XIa-PLA2) activity with an IC50 value 8.1 µg/ml. M. indica extract effectively inhibited the indirect hemolytic activity up to 98% at ~40 µg/ml concentration and at various concentrations (0-50 µg/ml), it dose dependently inhibited the edema formation. When examined as a function of increased substrate and calcium concentration, there was no relieve of inhibitory effect on the GIA sPLA2. Furthermore, the inhibition was irreversible as evidenced from binding studies. It is observed that the aqueous extract ofM. indica effectively inhibits sPLA2 and it is associated inflammatory activities, which substantiate their anti-inflammatory properties. The mode of inhibition could be due to direct interaction of components present in the extract, with sPLA2 enzyme. Further studies on understanding the principal constituents, responsible for the anti-inflammatory activity would be interesting to develop this into potent anti-inflammatory agent.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100197&lng=en&tlng=enfosfolipase A2 secretóriaanti-inflamatóriopró-inflamatórioinibiçãodrogasplantas |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
BHADRAPURA LAKKAPPA DHANANJAYA SUDHARSHAN SHIVALINGAIAH |
spellingShingle |
BHADRAPURA LAKKAPPA DHANANJAYA SUDHARSHAN SHIVALINGAIAH The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2 Anais da Academia Brasileira de Ciências fosfolipase A2 secretória anti-inflamatório pró-inflamatório inibição drogas plantas |
author_facet |
BHADRAPURA LAKKAPPA DHANANJAYA SUDHARSHAN SHIVALINGAIAH |
author_sort |
BHADRAPURA LAKKAPPA DHANANJAYA |
title |
The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2 |
title_short |
The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2 |
title_full |
The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2 |
title_fullStr |
The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2 |
title_full_unstemmed |
The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2 |
title_sort |
anti-inflammatory activity of standard aqueous stem bark extract of mangifera indica l. as evident in inhibition of group ia spla2 |
publisher |
Academia Brasileira de Ciências |
series |
Anais da Academia Brasileira de Ciências |
issn |
1678-2690 |
publishDate |
2016-03-01 |
description |
ABSTRACT The standard aqueous stem bark extract is consumed as herbal drink and used in the pharmaceutical formulations to treat patients suffering from various disease conditions in Cuba. This study was carried out to evaluate the modulatory effect of standard aqueous bark extract of M. indica on Group IA sPLA2. M. indica extract, dose dependently inhibited the GIA sPLA2 (NN-XIa-PLA2) activity with an IC50 value 8.1 µg/ml. M. indica extract effectively inhibited the indirect hemolytic activity up to 98% at ~40 µg/ml concentration and at various concentrations (0-50 µg/ml), it dose dependently inhibited the edema formation. When examined as a function of increased substrate and calcium concentration, there was no relieve of inhibitory effect on the GIA sPLA2. Furthermore, the inhibition was irreversible as evidenced from binding studies. It is observed that the aqueous extract ofM. indica effectively inhibits sPLA2 and it is associated inflammatory activities, which substantiate their anti-inflammatory properties. The mode of inhibition could be due to direct interaction of components present in the extract, with sPLA2 enzyme. Further studies on understanding the principal constituents, responsible for the anti-inflammatory activity would be interesting to develop this into potent anti-inflammatory agent. |
topic |
fosfolipase A2 secretória anti-inflamatório pró-inflamatório inibição drogas plantas |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100197&lng=en&tlng=en |
work_keys_str_mv |
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