Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5
Abstract Background Studies have demonstrated that long noncoding RNAs (lncRNAs) have essential impacts on the development of atherosclerosis (AS). This study aimed to identify the role and functional mechanism of lncRNA CASC2 in the development and migration of vascular smooth muscle cells (VSMCs)....
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doaj-77f096c7b25445049001eb4fa16365592020-11-25T02:56:03ZengBMCMolecular Medicine1076-15511528-36582020-07-012611910.1186/s10020-020-00200-3Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5Chenjing Wang0Jin Zhao1Xiaodong Nan2Zhong Guo3Shuangsheng Huang4Xiaokun Wang5Feng Sun6Shijie Ma7School of Basic Medical Sciences, Northwest Minzu University Health Science CenterSchool of Basic Medical Sciences, Northwest Minzu University Health Science CenterDepartment of intensive care unit, Gansu Provincial Corps Hospital of Chinese People’s Armed Police ForceSchool of Basic Medical Sciences, Northwest Minzu University Health Science CenterSchool of Basic Medical Sciences, Northwest Minzu University Health Science CenterSchool of Basic Medical Sciences, Northwest Minzu University Health Science CenterSchool of Basic Medical Sciences, Northwest Minzu University Health Science CenterSchool of Basic Medical Sciences, Northwest Minzu University Health Science CenterAbstract Background Studies have demonstrated that long noncoding RNAs (lncRNAs) have essential impacts on the development of atherosclerosis (AS). This study aimed to identify the role and functional mechanism of lncRNA CASC2 in the development and migration of vascular smooth muscle cells (VSMCs). Method The serum of 40 pairs of AS patients and healthy volunteers were collected and the expression of CASC2 was evaluated. qRT-PCR and western blotting were carried out to examine the expression levels of at mRNA and protein level, repectively. Cell proliferation assay, colony formation assay, transwell migration assay, dual-luciferase reporter assay, and wound healing assay were conducted to evaluate cell proliferation, colony formation, migration, transcription, targeting, and self-restoration. Results The expression levels of CASC2 were decreased, while the expression levels of miR-532-3p were elevated in AS patient samples and VSMCs. Overexpression of CASC2 inhibited the proliferation and migration of VSMCs and enhanced cell apoptosis. CASC2 inhibited the expression of miR-532-3p, and inversely upregulated the expression of PAPD5, which was a target of miR-532-3p. In addition, knockdown of miR-532-3p-mimic and PAPD5 could attenuate the impact of overexpression of CASC2 on proliferation, migration, and apoptosis in ox-LDL-VSMCs. Conclusion CASC2 suppressed cell reproduction and promoted cell apoptosis by regulating the miR-532-3p/PAPD5 axis in ox-LDL-mediated VSMCs. This might be important for AS therapeutics.http://link.springer.com/article/10.1186/s10020-020-00200-3CASC2AtherosclerosismiR-532-3pPAPD5 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chenjing Wang Jin Zhao Xiaodong Nan Zhong Guo Shuangsheng Huang Xiaokun Wang Feng Sun Shijie Ma |
spellingShingle |
Chenjing Wang Jin Zhao Xiaodong Nan Zhong Guo Shuangsheng Huang Xiaokun Wang Feng Sun Shijie Ma Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5 Molecular Medicine CASC2 Atherosclerosis miR-532-3p PAPD5 |
author_facet |
Chenjing Wang Jin Zhao Xiaodong Nan Zhong Guo Shuangsheng Huang Xiaokun Wang Feng Sun Shijie Ma |
author_sort |
Chenjing Wang |
title |
Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5 |
title_short |
Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5 |
title_full |
Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5 |
title_fullStr |
Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5 |
title_full_unstemmed |
Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5 |
title_sort |
long noncoding rna casc2 inhibits ox-ldl-mediated vascular smooth muscle cells proliferation and migration via the regulation of mir-532-3p/papd5 |
publisher |
BMC |
series |
Molecular Medicine |
issn |
1076-1551 1528-3658 |
publishDate |
2020-07-01 |
description |
Abstract Background Studies have demonstrated that long noncoding RNAs (lncRNAs) have essential impacts on the development of atherosclerosis (AS). This study aimed to identify the role and functional mechanism of lncRNA CASC2 in the development and migration of vascular smooth muscle cells (VSMCs). Method The serum of 40 pairs of AS patients and healthy volunteers were collected and the expression of CASC2 was evaluated. qRT-PCR and western blotting were carried out to examine the expression levels of at mRNA and protein level, repectively. Cell proliferation assay, colony formation assay, transwell migration assay, dual-luciferase reporter assay, and wound healing assay were conducted to evaluate cell proliferation, colony formation, migration, transcription, targeting, and self-restoration. Results The expression levels of CASC2 were decreased, while the expression levels of miR-532-3p were elevated in AS patient samples and VSMCs. Overexpression of CASC2 inhibited the proliferation and migration of VSMCs and enhanced cell apoptosis. CASC2 inhibited the expression of miR-532-3p, and inversely upregulated the expression of PAPD5, which was a target of miR-532-3p. In addition, knockdown of miR-532-3p-mimic and PAPD5 could attenuate the impact of overexpression of CASC2 on proliferation, migration, and apoptosis in ox-LDL-VSMCs. Conclusion CASC2 suppressed cell reproduction and promoted cell apoptosis by regulating the miR-532-3p/PAPD5 axis in ox-LDL-mediated VSMCs. This might be important for AS therapeutics. |
topic |
CASC2 Atherosclerosis miR-532-3p PAPD5 |
url |
http://link.springer.com/article/10.1186/s10020-020-00200-3 |
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