Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
Abstract Background Glioma is the most common primary central nervous system tumor derived from glial cells. Kininogen-1 (KNG1) can exert antiangiogenic properties and inhibit proliferation of endothelial cells. The effect of KNG1 on the glioma is rarely reported, so our purpose in to explore its me...
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doaj-77f2d17b259041d5b6898007bedc640d2020-11-25T02:03:07ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662018-08-0137111510.1186/s13046-018-0833-0Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cellsJinfang Xu0Jun Fang1Zhonghao Cheng2Longlong Fan3Weiwei Hu4Feng Zhou5Hong Shen6Department of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of MedicineDepartment of Radiotherapy, Zhejiang Cancer HospitalDepartment of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of MedicineDepartment of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of MedicineDepartment of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of MedicineDepartment of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of MedicineDepartment of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of MedicineAbstract Background Glioma is the most common primary central nervous system tumor derived from glial cells. Kininogen-1 (KNG1) can exert antiangiogenic properties and inhibit proliferation of endothelial cells. The effect of KNG1 on the glioma is rarely reported, so our purpose in to explore its mechanism in glioma cells. Methods The differentially expressed genes (DEGs) were identified based on The Cancer Genome Atlas (TCGA) database. The KNG1-vector was transfected into the two glioma cells. The viability, apoptosis and cell cycle of glioma cells and microvessel density (MVD) were detected by cell counting kit-8 assay, flow cytometry and immunohistochemistry, respectively. The expression were measured by quantitative real-time PCR and Western blot, respectively. A tumor mouse model was established to determine apoptosis rate of brain tissue by terminal deoxynucleotidyl transfer-mediated dUTP nick end labeling (TUNEL) analysis. Results KNG1 was identified as the core gene and lowly expressed in the glioma cells. Overexpression of KNG1 inhibited cell viability and angiogenesis of glioma cells. Overexpression of KNG1 promoted the apoptosis and G1 phase cell cycle arrest of glioma cells. Moreover, the expressions of VEGF, cyclinD1, ki67, caspase-3/9 and XIAP were regulated by overexpression of KNG1. In addition, overexpression of KNG1 inhibited the activity of PI3K/Akt. Furthermore, overexpression of KNG1 decreased the tumor growth and promoted the apoptosis of decreased by overexpression of KNG1 in vivo. . Conclusions Overexpression of KNG1 suppresses glioma progression by inhibiting the proliferation and promoting apoptosis of glioma cells, providing a therapeutic strategy for the malignant glioma.http://link.springer.com/article/10.1186/s13046-018-0833-0KNG1GliomaApoptosisAngiogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jinfang Xu Jun Fang Zhonghao Cheng Longlong Fan Weiwei Hu Feng Zhou Hong Shen |
spellingShingle |
Jinfang Xu Jun Fang Zhonghao Cheng Longlong Fan Weiwei Hu Feng Zhou Hong Shen Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells Journal of Experimental & Clinical Cancer Research KNG1 Glioma Apoptosis Angiogenesis |
author_facet |
Jinfang Xu Jun Fang Zhonghao Cheng Longlong Fan Weiwei Hu Feng Zhou Hong Shen |
author_sort |
Jinfang Xu |
title |
Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells |
title_short |
Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells |
title_full |
Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells |
title_fullStr |
Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells |
title_full_unstemmed |
Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells |
title_sort |
overexpression of the kininogen-1 inhibits proliferation and induces apoptosis of glioma cells |
publisher |
BMC |
series |
Journal of Experimental & Clinical Cancer Research |
issn |
1756-9966 |
publishDate |
2018-08-01 |
description |
Abstract Background Glioma is the most common primary central nervous system tumor derived from glial cells. Kininogen-1 (KNG1) can exert antiangiogenic properties and inhibit proliferation of endothelial cells. The effect of KNG1 on the glioma is rarely reported, so our purpose in to explore its mechanism in glioma cells. Methods The differentially expressed genes (DEGs) were identified based on The Cancer Genome Atlas (TCGA) database. The KNG1-vector was transfected into the two glioma cells. The viability, apoptosis and cell cycle of glioma cells and microvessel density (MVD) were detected by cell counting kit-8 assay, flow cytometry and immunohistochemistry, respectively. The expression were measured by quantitative real-time PCR and Western blot, respectively. A tumor mouse model was established to determine apoptosis rate of brain tissue by terminal deoxynucleotidyl transfer-mediated dUTP nick end labeling (TUNEL) analysis. Results KNG1 was identified as the core gene and lowly expressed in the glioma cells. Overexpression of KNG1 inhibited cell viability and angiogenesis of glioma cells. Overexpression of KNG1 promoted the apoptosis and G1 phase cell cycle arrest of glioma cells. Moreover, the expressions of VEGF, cyclinD1, ki67, caspase-3/9 and XIAP were regulated by overexpression of KNG1. In addition, overexpression of KNG1 inhibited the activity of PI3K/Akt. Furthermore, overexpression of KNG1 decreased the tumor growth and promoted the apoptosis of decreased by overexpression of KNG1 in vivo. . Conclusions Overexpression of KNG1 suppresses glioma progression by inhibiting the proliferation and promoting apoptosis of glioma cells, providing a therapeutic strategy for the malignant glioma. |
topic |
KNG1 Glioma Apoptosis Angiogenesis |
url |
http://link.springer.com/article/10.1186/s13046-018-0833-0 |
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