The role of ADAMTS genes in the end stage of hip osteoarthritis
Purpose: The aim of this study is to investigate which ADAMTS genes play a major role in the development of primary hip osteoarthritis, by comparing the tissue and blood samples in patients with hip osteoarthritis and a control group. Material and methods: Human articular cartilage was obtained from...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
AVES Yayincilik
2019-03-01
|
Series: | Acta Orthopaedica et Traumatologica Turcica |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1017995X18301688 |
id |
doaj-7803112a433b4b689b475d103644d523 |
---|---|
record_format |
Article |
spelling |
doaj-7803112a433b4b689b475d103644d5232020-11-25T02:47:41ZengAVES YayincilikActa Orthopaedica et Traumatologica Turcica1017-995X2019-03-01532140144The role of ADAMTS genes in the end stage of hip osteoarthritisTacettin Ayanoglu0Hakan Atalar1Erdinç Esen2Muhammet Baybars Ataoğlu3Sacit Turanlı4Kadir Demircan5Department of Orthopaedics and Traumatology, Yozgat City Hospital, Yozgat, Turkey; Corresponding author. Department of Orthopedics and Traumatology, Yozgat City Hospital, 66100, Yozgat, Turkey. Fax: +903122129008.Department of Orthopedics and Traumatology, Gazi University Faculty of Medicine, Ankara, TurkeyDepartment of Orthopedics and Traumatology, Gazi University Faculty of Medicine, Ankara, TurkeyDepartment of Orthopedics and Traumatology, Gazi University Faculty of Medicine, Ankara, TurkeyDepartment of Orthopedics and Traumatology, Gazi University Faculty of Medicine, Ankara, TurkeyDepartment of Medical Biology, Gazi University Faculty of Medicine, Ankara, TurkeyPurpose: The aim of this study is to investigate which ADAMTS genes play a major role in the development of primary hip osteoarthritis, by comparing the tissue and blood samples in patients with hip osteoarthritis and a control group. Material and methods: Human articular cartilage was obtained from femoral heads of 15 patients with end stage osteoarthritis undergoing total hip replacement. As the control group, the cartilages was obtained from femoral heads of 15 patients, who did not have osteoarthritis or degenerative changes in hip joint, undergoing hip replacement following the fracture of the femoral neck. After the cartilage samples were taken from the resection materials, the DNA polymorphisms in the patients' cartilage samples were tested by Polymerase Chain Reaction (PCR), the serum levels of aggrecanase genes were analyzed with Enzyme-Linked ImmunoSorbent Assay (ELISA). Results: The level of ADAMTS5 and ADAMTS9 genes were found significantly lower as a result of ELISA analysis degenerative arthritis group than the control group (p < 0,05). ADAMTS 1, 4, 8, 15 were similar between the two groups in ELISA analysis (p > 0,05). As a result of quantitative real time RT-PCR analysis, the level of ADAMTS8 mRNA increased 3.5 fold in hip degenerative arthritis group when compared with femoral neck fractures group. ADAMTS1, ADAMTS4 and ADAMTS5 expression levels in hip degenerative arthritis group were decreased 2.5, 2 and 2.5 fold, respectively. ADAMTS9, 15 were found to be similar between two groups. Concluson: As a result of this study on hip osteoarthritis, the ADAMTS8 levels was found to be significantly higher in the end stage of hip osteoarthritis. Unlike similar studies on knee osteoarthritis, ADAMTS1,4,5 levels were found to be lower. Keywords: ADAMTS genes, Osteoarthritis, Genetic, Hip, PCR, ELISAhttp://www.sciencedirect.com/science/article/pii/S1017995X18301688 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tacettin Ayanoglu Hakan Atalar Erdinç Esen Muhammet Baybars Ataoğlu Sacit Turanlı Kadir Demircan |
spellingShingle |
Tacettin Ayanoglu Hakan Atalar Erdinç Esen Muhammet Baybars Ataoğlu Sacit Turanlı Kadir Demircan The role of ADAMTS genes in the end stage of hip osteoarthritis Acta Orthopaedica et Traumatologica Turcica |
author_facet |
Tacettin Ayanoglu Hakan Atalar Erdinç Esen Muhammet Baybars Ataoğlu Sacit Turanlı Kadir Demircan |
author_sort |
Tacettin Ayanoglu |
title |
The role of ADAMTS genes in the end stage of hip osteoarthritis |
title_short |
The role of ADAMTS genes in the end stage of hip osteoarthritis |
title_full |
The role of ADAMTS genes in the end stage of hip osteoarthritis |
title_fullStr |
The role of ADAMTS genes in the end stage of hip osteoarthritis |
title_full_unstemmed |
The role of ADAMTS genes in the end stage of hip osteoarthritis |
title_sort |
role of adamts genes in the end stage of hip osteoarthritis |
publisher |
AVES Yayincilik |
series |
Acta Orthopaedica et Traumatologica Turcica |
issn |
1017-995X |
publishDate |
2019-03-01 |
description |
Purpose: The aim of this study is to investigate which ADAMTS genes play a major role in the development of primary hip osteoarthritis, by comparing the tissue and blood samples in patients with hip osteoarthritis and a control group. Material and methods: Human articular cartilage was obtained from femoral heads of 15 patients with end stage osteoarthritis undergoing total hip replacement. As the control group, the cartilages was obtained from femoral heads of 15 patients, who did not have osteoarthritis or degenerative changes in hip joint, undergoing hip replacement following the fracture of the femoral neck. After the cartilage samples were taken from the resection materials, the DNA polymorphisms in the patients' cartilage samples were tested by Polymerase Chain Reaction (PCR), the serum levels of aggrecanase genes were analyzed with Enzyme-Linked ImmunoSorbent Assay (ELISA). Results: The level of ADAMTS5 and ADAMTS9 genes were found significantly lower as a result of ELISA analysis degenerative arthritis group than the control group (p < 0,05). ADAMTS 1, 4, 8, 15 were similar between the two groups in ELISA analysis (p > 0,05). As a result of quantitative real time RT-PCR analysis, the level of ADAMTS8 mRNA increased 3.5 fold in hip degenerative arthritis group when compared with femoral neck fractures group. ADAMTS1, ADAMTS4 and ADAMTS5 expression levels in hip degenerative arthritis group were decreased 2.5, 2 and 2.5 fold, respectively. ADAMTS9, 15 were found to be similar between two groups. Concluson: As a result of this study on hip osteoarthritis, the ADAMTS8 levels was found to be significantly higher in the end stage of hip osteoarthritis. Unlike similar studies on knee osteoarthritis, ADAMTS1,4,5 levels were found to be lower. Keywords: ADAMTS genes, Osteoarthritis, Genetic, Hip, PCR, ELISA |
url |
http://www.sciencedirect.com/science/article/pii/S1017995X18301688 |
work_keys_str_mv |
AT tacettinayanoglu theroleofadamtsgenesintheendstageofhiposteoarthritis AT hakanatalar theroleofadamtsgenesintheendstageofhiposteoarthritis AT erdincesen theroleofadamtsgenesintheendstageofhiposteoarthritis AT muhammetbaybarsataoglu theroleofadamtsgenesintheendstageofhiposteoarthritis AT sacitturanlı theroleofadamtsgenesintheendstageofhiposteoarthritis AT kadirdemircan theroleofadamtsgenesintheendstageofhiposteoarthritis AT tacettinayanoglu roleofadamtsgenesintheendstageofhiposteoarthritis AT hakanatalar roleofadamtsgenesintheendstageofhiposteoarthritis AT erdincesen roleofadamtsgenesintheendstageofhiposteoarthritis AT muhammetbaybarsataoglu roleofadamtsgenesintheendstageofhiposteoarthritis AT sacitturanlı roleofadamtsgenesintheendstageofhiposteoarthritis AT kadirdemircan roleofadamtsgenesintheendstageofhiposteoarthritis |
_version_ |
1724751956607500288 |