Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial Infarction
Cell therapy raises hope to reduce the harmful effects of acute myocardial ischemia. Stem and progenitor cells (SPCs) may be a valuable source of trophic factors. In this study, we assessed the plasma levels of selected trophic factors in patients undergoing application of autologous bone marrow (BM...
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doaj-782c8266c6e747c88579a5cc4c3cfaf82020-11-24T21:36:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-07-012013333010.3390/ijms20133330ijms20133330Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial InfarctionBartłomiej Baumert0Krzysztof Przybycień1Edyta Paczkowska2Maciej Kotowski3Ewa Pius-Sadowska4Krzysztof Safranow5Jarosław Peregud-Pogorzelski6Zdzisława Kornacewicz-Jach7Małgorzata Peregud-Pogorzelska8Bogusław Machaliński9Department of General Pathology, Pomeranian Medical University, 71-111 Szczecin, PolandDepartment of Cardiology, Pomeranian Medical University, 71-111 Szczecin, PolandDepartment of General Pathology, Pomeranian Medical University, 71-111 Szczecin, PolandDepartment of General Pathology, Pomeranian Medical University, 71-111 Szczecin, PolandDepartment of General Pathology, Pomeranian Medical University, 71-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University, 71-111 Szczecin, PolandDepartment of Paediatric Oncology, Pomeranian Medical University, 71-252 Szczecin, PolandDepartment of Cardiology, Pomeranian Medical University, 71-111 Szczecin, PolandDepartment of Cardiology, Pomeranian Medical University, 71-111 Szczecin, PolandDepartment of General Pathology, Pomeranian Medical University, 71-111 Szczecin, PolandCell therapy raises hope to reduce the harmful effects of acute myocardial ischemia. Stem and progenitor cells (SPCs) may be a valuable source of trophic factors. In this study, we assessed the plasma levels of selected trophic factors in patients undergoing application of autologous bone marrow (BM)-derived, lineage-negative (Lin<sup>−</sup>) stem/progenitor cells into the coronary artery in the acute phase of myocardial infarction. The study group consisted of 15 patients with acute myocardial infarction (AMI) who underwent percutaneous revascularization and, afterwards, Lin<sup>−</sup> stem/progenitor cell administration into the infarct-related artery. The control group consisted of 19 patients. BM Lin<sup>−</sup> cells were isolated using immunomagnetic methods. Peripheral blood was collected on day 0, 2, 4, and 7 and after the first and third month to assess the concentration of selected trophic factors using multiplex fluorescent bead-based immunoassays. We found in the Lin<sup>−</sup> group that several angiogenic trophic factors (vascular endothelial growth factor, Angiopoietin-1, basic fibroblast growth factor, platelet-derived growth factor-aa) plasma level significantly increased to the 4th day after myocardial infarction. In parallel, we noticed a tendency where the plasma levels of the brain-derived neurotrophic factor were increased in the Lin<sup>–</sup> group. The obtained results suggest that the administered SPCs may be a valuable source of angiogenic trophic factors for damaged myocardium, although this observation requires further in-depth studies.https://www.mdpi.com/1422-0067/20/13/3330angiogenic trophic factorsneurotrophinscell therapymyocardial infarctionstem cells |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bartłomiej Baumert Krzysztof Przybycień Edyta Paczkowska Maciej Kotowski Ewa Pius-Sadowska Krzysztof Safranow Jarosław Peregud-Pogorzelski Zdzisława Kornacewicz-Jach Małgorzata Peregud-Pogorzelska Bogusław Machaliński |
spellingShingle |
Bartłomiej Baumert Krzysztof Przybycień Edyta Paczkowska Maciej Kotowski Ewa Pius-Sadowska Krzysztof Safranow Jarosław Peregud-Pogorzelski Zdzisława Kornacewicz-Jach Małgorzata Peregud-Pogorzelska Bogusław Machaliński Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial Infarction International Journal of Molecular Sciences angiogenic trophic factors neurotrophins cell therapy myocardial infarction stem cells |
author_facet |
Bartłomiej Baumert Krzysztof Przybycień Edyta Paczkowska Maciej Kotowski Ewa Pius-Sadowska Krzysztof Safranow Jarosław Peregud-Pogorzelski Zdzisława Kornacewicz-Jach Małgorzata Peregud-Pogorzelska Bogusław Machaliński |
author_sort |
Bartłomiej Baumert |
title |
Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial Infarction |
title_short |
Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial Infarction |
title_full |
Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial Infarction |
title_fullStr |
Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial Infarction |
title_full_unstemmed |
Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial Infarction |
title_sort |
novel evidence of the increase in angiogenic factor plasma levels after lineage-negative stem/progenitor cell intracoronary infusion in patients with acute myocardial infarction |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-07-01 |
description |
Cell therapy raises hope to reduce the harmful effects of acute myocardial ischemia. Stem and progenitor cells (SPCs) may be a valuable source of trophic factors. In this study, we assessed the plasma levels of selected trophic factors in patients undergoing application of autologous bone marrow (BM)-derived, lineage-negative (Lin<sup>−</sup>) stem/progenitor cells into the coronary artery in the acute phase of myocardial infarction. The study group consisted of 15 patients with acute myocardial infarction (AMI) who underwent percutaneous revascularization and, afterwards, Lin<sup>−</sup> stem/progenitor cell administration into the infarct-related artery. The control group consisted of 19 patients. BM Lin<sup>−</sup> cells were isolated using immunomagnetic methods. Peripheral blood was collected on day 0, 2, 4, and 7 and after the first and third month to assess the concentration of selected trophic factors using multiplex fluorescent bead-based immunoassays. We found in the Lin<sup>−</sup> group that several angiogenic trophic factors (vascular endothelial growth factor, Angiopoietin-1, basic fibroblast growth factor, platelet-derived growth factor-aa) plasma level significantly increased to the 4th day after myocardial infarction. In parallel, we noticed a tendency where the plasma levels of the brain-derived neurotrophic factor were increased in the Lin<sup>–</sup> group. The obtained results suggest that the administered SPCs may be a valuable source of angiogenic trophic factors for damaged myocardium, although this observation requires further in-depth studies. |
topic |
angiogenic trophic factors neurotrophins cell therapy myocardial infarction stem cells |
url |
https://www.mdpi.com/1422-0067/20/13/3330 |
work_keys_str_mv |
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