Myroides odoratimimus Forms Structurally Complex and Inherently Antibiotic-Resistant Biofilm in a Wound-Like in vitro Model
Myroides odoratimimus is an aerobic, non-fermenting Gram-negative multidrug-resistant bacterium widely distributed in nature that rarely causes infections in immunocompromised patients. We recently described in a diabetic patient a case of recurrent calcaneal ulcer infection caused by a M. odoratimi...
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doaj-7832bfe7d05544a28c509db811c8b9e52020-11-25T00:04:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-12-01810.3389/fmicb.2017.02591322335Myroides odoratimimus Forms Structurally Complex and Inherently Antibiotic-Resistant Biofilm in a Wound-Like in vitro ModelArianna Pompilio0Arianna Pompilio1Giuseppe Galardi2Giuseppe Galardi3Fabio Verginelli4Fabio Verginelli5Maurizio Muzzi6Andrea Di Giulio7Giovanni Di Bonaventura8Giovanni Di Bonaventura9Department of Medical, Oral & Biotechnological Sciences, “G. d'Annunzio” University of Chieti-Pescara, Chieti, ItalyAging Research Center and Translational Medicine, “G. d'Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Medical, Oral & Biotechnological Sciences, “G. d'Annunzio” University of Chieti-Pescara, Chieti, ItalyAging Research Center and Translational Medicine, “G. d'Annunzio” University of Chieti-Pescara, Chieti, ItalyAging Research Center and Translational Medicine, “G. d'Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Pharmacy, “G. d'Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Science, LIME, University of Roma Tre, Rome, ItalyDepartment of Science, LIME, University of Roma Tre, Rome, ItalyDepartment of Medical, Oral & Biotechnological Sciences, “G. d'Annunzio” University of Chieti-Pescara, Chieti, ItalyAging Research Center and Translational Medicine, “G. d'Annunzio” University of Chieti-Pescara, Chieti, ItalyMyroides odoratimimus is an aerobic, non-fermenting Gram-negative multidrug-resistant bacterium widely distributed in nature that rarely causes infections in immunocompromised patients. We recently described in a diabetic patient a case of recurrent calcaneal ulcer infection caused by a M. odoratimimus strain showing potential for biofilm formation. For the first time, we therefore evaluated the ability of M. odoratimimus to form biofilm under different pH values and glucose concentrations using an in vitro “skin-like” model, and its susceptibility to levofloxacin, meropenem, and tigecycline. The expression of some antibiotic-resistance related genes was also monitored by RT-PCR during planktonic-to-biofilm transition. Our results indicated that M. odoratimimus can produce relevant amounts of biofilm biomass, in a time-dependent manner, especially at acidic pH and regardless of glucose concentration tested. The comparative analysis of MIC and MBC values between planktonic and sessile cells showed that resistance to antibiotics increased during the planktonic-to-biofilm transition. Viable cell count indicated that none of the tested antibiotics were able to completely eradicate preformed biofilms, although meropenem and levofloxacin were the most active causing a significant, dose-independent, reduction of biofilm's viability, as also confirmed by microscopic analysis. RT-PCR showed that antibiotic-resistance related gyrA and acrB genes are over-expressed during the transition from planktonic to sessile (biofilm) lifestyle. Overall, our findings showed that M. odoratimimus can form relevant amounts of inherently antibiotic-resistant biofilm under conditions relevant to wound site, therefore suggesting a role in the pathogenesis of chronic ulcer infections.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02591/fullMyroides odoratimimusantibiotic-resistancebiofilmwound-like in vitro modelrecurrent ulcer infection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arianna Pompilio Arianna Pompilio Giuseppe Galardi Giuseppe Galardi Fabio Verginelli Fabio Verginelli Maurizio Muzzi Andrea Di Giulio Giovanni Di Bonaventura Giovanni Di Bonaventura |
spellingShingle |
Arianna Pompilio Arianna Pompilio Giuseppe Galardi Giuseppe Galardi Fabio Verginelli Fabio Verginelli Maurizio Muzzi Andrea Di Giulio Giovanni Di Bonaventura Giovanni Di Bonaventura Myroides odoratimimus Forms Structurally Complex and Inherently Antibiotic-Resistant Biofilm in a Wound-Like in vitro Model Frontiers in Microbiology Myroides odoratimimus antibiotic-resistance biofilm wound-like in vitro model recurrent ulcer infection |
author_facet |
Arianna Pompilio Arianna Pompilio Giuseppe Galardi Giuseppe Galardi Fabio Verginelli Fabio Verginelli Maurizio Muzzi Andrea Di Giulio Giovanni Di Bonaventura Giovanni Di Bonaventura |
author_sort |
Arianna Pompilio |
title |
Myroides odoratimimus Forms Structurally Complex and Inherently Antibiotic-Resistant Biofilm in a Wound-Like in vitro Model |
title_short |
Myroides odoratimimus Forms Structurally Complex and Inherently Antibiotic-Resistant Biofilm in a Wound-Like in vitro Model |
title_full |
Myroides odoratimimus Forms Structurally Complex and Inherently Antibiotic-Resistant Biofilm in a Wound-Like in vitro Model |
title_fullStr |
Myroides odoratimimus Forms Structurally Complex and Inherently Antibiotic-Resistant Biofilm in a Wound-Like in vitro Model |
title_full_unstemmed |
Myroides odoratimimus Forms Structurally Complex and Inherently Antibiotic-Resistant Biofilm in a Wound-Like in vitro Model |
title_sort |
myroides odoratimimus forms structurally complex and inherently antibiotic-resistant biofilm in a wound-like in vitro model |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-12-01 |
description |
Myroides odoratimimus is an aerobic, non-fermenting Gram-negative multidrug-resistant bacterium widely distributed in nature that rarely causes infections in immunocompromised patients. We recently described in a diabetic patient a case of recurrent calcaneal ulcer infection caused by a M. odoratimimus strain showing potential for biofilm formation. For the first time, we therefore evaluated the ability of M. odoratimimus to form biofilm under different pH values and glucose concentrations using an in vitro “skin-like” model, and its susceptibility to levofloxacin, meropenem, and tigecycline. The expression of some antibiotic-resistance related genes was also monitored by RT-PCR during planktonic-to-biofilm transition. Our results indicated that M. odoratimimus can produce relevant amounts of biofilm biomass, in a time-dependent manner, especially at acidic pH and regardless of glucose concentration tested. The comparative analysis of MIC and MBC values between planktonic and sessile cells showed that resistance to antibiotics increased during the planktonic-to-biofilm transition. Viable cell count indicated that none of the tested antibiotics were able to completely eradicate preformed biofilms, although meropenem and levofloxacin were the most active causing a significant, dose-independent, reduction of biofilm's viability, as also confirmed by microscopic analysis. RT-PCR showed that antibiotic-resistance related gyrA and acrB genes are over-expressed during the transition from planktonic to sessile (biofilm) lifestyle. Overall, our findings showed that M. odoratimimus can form relevant amounts of inherently antibiotic-resistant biofilm under conditions relevant to wound site, therefore suggesting a role in the pathogenesis of chronic ulcer infections. |
topic |
Myroides odoratimimus antibiotic-resistance biofilm wound-like in vitro model recurrent ulcer infection |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.02591/full |
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