Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley

To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources great...

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Main Authors:	Ruiju Lu, Zhiwei Chen, Runhong Gao, Ting He, Yifei Wang, Hongwei Xu, Guimei Guo, Yingbo Li, Chenghong Liu, Jianhua Huang
Format:	Article
Language:	English
Published:	Hindawi Limited 2016-01-01
Series:	BioMed Research International
Online Access:	http://dx.doi.org/10.1155/2016/1801646

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record_format	Article
spelling	doaj-788c1ff36d5c4655a13098d53fe5e7522020-11-24T22:29:07ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/18016461801646Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in BarleyRuiju Lu0Zhiwei Chen1Runhong Gao2Ting He3Yifei Wang4Hongwei Xu5Guimei Guo6Yingbo Li7Chenghong Liu8Jianhua Huang9Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaTo establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would significantly decrease the callus induction. And the further optimization experiments revealed that the increasing of organic nitrogen sources was much important in callus induction while it seemed different in plant regeneration. Based on the great effects of organic nitrogen on callus induction, the medium of N6-ANO1/4-2000 might be the best choice for the microspore culture system. In addition, the phylogenetic analysis indicated that there were clear differences of genetic backgrounds among these barley genotypes, and it also suggested that this medium for microspore culture had widespread utilization in different barley genotypes.http://dx.doi.org/10.1155/2016/1801646
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ruiju Lu Zhiwei Chen Runhong Gao Ting He Yifei Wang Hongwei Xu Guimei Guo Yingbo Li Chenghong Liu Jianhua Huang
spellingShingle	Ruiju Lu Zhiwei Chen Runhong Gao Ting He Yifei Wang Hongwei Xu Guimei Guo Yingbo Li Chenghong Liu Jianhua Huang Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley BioMed Research International
author_facet	Ruiju Lu Zhiwei Chen Runhong Gao Ting He Yifei Wang Hongwei Xu Guimei Guo Yingbo Li Chenghong Liu Jianhua Huang
author_sort	Ruiju Lu
title	Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley
title_short	Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley
title_full	Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley
title_fullStr	Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley
title_full_unstemmed	Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley
title_sort	genotypes-independent optimization of nitrogen supply for isolated microspore cultures in barley
publisher	Hindawi Limited
series	BioMed Research International
issn	2314-6133 2314-6141
publishDate	2016-01-01
description	To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would significantly decrease the callus induction. And the further optimization experiments revealed that the increasing of organic nitrogen sources was much important in callus induction while it seemed different in plant regeneration. Based on the great effects of organic nitrogen on callus induction, the medium of N6-ANO1/4-2000 might be the best choice for the microspore culture system. In addition, the phylogenetic analysis indicated that there were clear differences of genetic backgrounds among these barley genotypes, and it also suggested that this medium for microspore culture had widespread utilization in different barley genotypes.
url	http://dx.doi.org/10.1155/2016/1801646
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Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley

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