Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder Carcinomas
Neo-angiogenesis may have an important role in the poor prognosis of gallbladder carcinoma. An enhanced expression of COX-2 was found in precancerous lesions and in gallbladder carcinoma, likely to be involved in carcinogenesis as well as in angiogenesis. To study the relationships between the COX-2...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2006-10-01
|
Series: | Bosnian Journal of Basic Medical Sciences |
Subjects: | |
Online Access: | http://www.bjbms.org/ojs/index.php/bjbms/article/view/3122 |
id |
doaj-789ac273289848478086bc9fd3c8d0a8 |
---|---|
record_format |
Article |
spelling |
doaj-789ac273289848478086bc9fd3c8d0a82020-11-24T21:35:10ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBosnian Journal of Basic Medical Sciences1512-86011840-48122006-10-016410.17305/bjbms.2006.3122682Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder CarcinomasMateja Legan0Boštjan Luzar1Vera Ferlan-Marolt2Andrej Cör3Institute of Histology and Embryology, Faculty of Medicine, University of LjubljanaInstitute of Pathology, Faculty of Medicine, University of LjubljanaInstitute of Pathology, Faculty of Medicine, University of LjubljanaInstitute of Histology and Embryology, Faculty of Medicine, University of LjubljanaNeo-angiogenesis may have an important role in the poor prognosis of gallbladder carcinoma. An enhanced expression of COX-2 was found in precancerous lesions and in gallbladder carcinoma, likely to be involved in carcinogenesis as well as in angiogenesis. To study the relationships between the COX-2 expression and degree of vascularization, as well as to evaluate their role in the prognosis of patients with gallbladder carcinoma. 27 cases of gallbladder adenocarcinoma were included, classified grading I-III according the WHO classification. The COX-2 and endothelial antigen CD105 expressions were assessed immunohistochemically. COX-2 expression was evaluated according to the percentage and staining intensity of positive cells into "COX-2 positive" and "COX-2 negative" groups. In order to assess tumor microvessel density (MVD), CD105 positively stained microvessels were counted for each specimen in predominantly vascular areas (hot spots) at 200 x magnification. The MVD ranged from 9 to 46 microvessels/field. 15 tumors belonged to the hypervascular group (MVD > or = 25) and 12 to the hypovascular group. There were 16 (59.2%) COX-2 positive cases. There was difference in the degree of angiogenesis between COX-2 positive vs. COX-2 negative group: 11 (68.8%) out of 16 "COX-2 positive" tumors were hypervascular, in comparison with just 4 (36.4%) of "COX-2 negative" tumors. Our data show that the MVD corresponds to the COX-2 overexpression in gallbladder carcinomas. Augmented tumor neovascularization induced by COX-2 might be responsible for the poor prognosis in gallbladder carcinoma patients.http://www.bjbms.org/ojs/index.php/bjbms/article/view/3122gallbladder carcinomacyclooxygenasetumor angiogenesisendoglinintratumoral microvessel density |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mateja Legan Boštjan Luzar Vera Ferlan-Marolt Andrej Cör |
spellingShingle |
Mateja Legan Boštjan Luzar Vera Ferlan-Marolt Andrej Cör Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder Carcinomas Bosnian Journal of Basic Medical Sciences gallbladder carcinoma cyclooxygenase tumor angiogenesis endoglin intratumoral microvessel density |
author_facet |
Mateja Legan Boštjan Luzar Vera Ferlan-Marolt Andrej Cör |
author_sort |
Mateja Legan |
title |
Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder Carcinomas |
title_short |
Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder Carcinomas |
title_full |
Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder Carcinomas |
title_fullStr |
Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder Carcinomas |
title_full_unstemmed |
Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder Carcinomas |
title_sort |
cyclooxygenase-2 expression determines neoangiogenesis in gallbladder carcinomas |
publisher |
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
series |
Bosnian Journal of Basic Medical Sciences |
issn |
1512-8601 1840-4812 |
publishDate |
2006-10-01 |
description |
Neo-angiogenesis may have an important role in the poor prognosis of gallbladder carcinoma. An enhanced expression of COX-2 was found in precancerous lesions and in gallbladder carcinoma, likely to be involved in carcinogenesis as well as in angiogenesis. To study the relationships between the COX-2 expression and degree of vascularization, as well as to evaluate their role in the prognosis of patients with gallbladder carcinoma. 27 cases of gallbladder adenocarcinoma were included, classified grading I-III according the WHO classification. The COX-2 and endothelial antigen CD105 expressions were assessed immunohistochemically. COX-2 expression was evaluated according to the percentage and staining intensity of positive cells into "COX-2 positive" and "COX-2 negative" groups. In order to assess tumor microvessel density (MVD), CD105 positively stained microvessels were counted for each specimen in predominantly vascular areas (hot spots) at 200 x magnification. The MVD ranged from 9 to 46 microvessels/field. 15 tumors belonged to the hypervascular group (MVD > or = 25) and 12 to the hypovascular group. There were 16 (59.2%) COX-2 positive cases. There was difference in the degree of angiogenesis between COX-2 positive vs. COX-2 negative group: 11 (68.8%) out of 16 "COX-2 positive" tumors were hypervascular, in comparison with just 4 (36.4%) of "COX-2 negative" tumors. Our data show that the MVD corresponds to the COX-2 overexpression in gallbladder carcinomas. Augmented tumor neovascularization induced by COX-2 might be responsible for the poor prognosis in gallbladder carcinoma patients. |
topic |
gallbladder carcinoma cyclooxygenase tumor angiogenesis endoglin intratumoral microvessel density |
url |
http://www.bjbms.org/ojs/index.php/bjbms/article/view/3122 |
work_keys_str_mv |
AT matejalegan cyclooxygenase2expressiondeterminesneoangiogenesisingallbladdercarcinomas AT bostjanluzar cyclooxygenase2expressiondeterminesneoangiogenesisingallbladdercarcinomas AT veraferlanmarolt cyclooxygenase2expressiondeterminesneoangiogenesisingallbladdercarcinomas AT andrejcor cyclooxygenase2expressiondeterminesneoangiogenesisingallbladdercarcinomas |
_version_ |
1725946252426739712 |