Effect of Type I Diabetes on the Proteome of Mouse Oocytes

• Main Authors: Guangjian Jiang, Guangli Zhang, Tian An, Zhongchen He, Lihua Kang, Xiuyan Yang, Yujie Gu, Dongwei Zhang, Yangang Wang, Sihua Gao
• Format: Article
• Language: English
• Published: Cell Physiol Biochem Press GmbH & Co KG 2016-11-01
• Series: Cellular Physiology and Biochemistry
• Subjects: Oocyte; Type I diabetes; Histone variant; Metabolism; Streptozotocin
• Online Access: http://www.karger.com/Article/FullText/447924

Background: Type I diabetes is a global public health concern that affects young people of reproductive age and can damage oocytes, reducing their maturation rate and blocking embryonic development. Understanding the effects of type I diabetes on oocytes is important to facilitate the maintenance of reproductive capacity in female diabetic patients. Methods: To analyze the effects of type I diabetes on mammalian oocytes, protein profile changes in mice with streptozotocin-induced type I diabetes were investigated using proteomic tools; non-diabetic mouse oocytes were used as controls. Immunofluorescence analysis for the spindle and mitochondria of oocytes. Results: We found that type I diabetes severely disturbed the metabolic processes of mouse oocytes. We also observed significant changes in levels of histone H1, H2A/B, and H3 variants in diabetic oocytes (fold change: > 0.4 or Conclusion: Our results indicate that type I diabetes disrupts metabolic processes, spindle formation, mitochondria distribution and modulates epigenetic code in oocytes. Such effects could have a major impact on the reproductive dynamics of female patients with type I diabetes.