Microfluidics-Based Single-Cell Research for Intercellular Interaction
Intercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophis...
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Frontiers Media S.A.
2021-08-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.680307/full |
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doaj-78b9ba935aa9457b8e14db91720a02312021-08-12T09:23:11ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-08-01910.3389/fcell.2021.680307680307Microfluidics-Based Single-Cell Research for Intercellular InteractionLong Pang0Long Pang1Jing Ding2Xi-Xian Liu3Zhixuan Kou4Zhixuan Kou5Lulu Guo6Lulu Guo7Xi Xu8Xi Xu9Shih-Kang Fan10School of Basic Medical Science, The Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, ChinaXi’an Key Laboratory of Pathogenic Microorganism and Tumor Immunity, Xi’an Medical University, Xi’an, ChinaDepartment of Mechanical and Nuclear Engineering, Kansas State University, Manhattan, KS, United StatesKey Laboratory of Thermo-Fluid Science and Engineering of MOE, School of Energy and Power Engineering, Xi’an Jiaotong University, Xi’an, ChinaSchool of Basic Medical Science, The Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, ChinaXi’an Key Laboratory of Pathogenic Microorganism and Tumor Immunity, Xi’an Medical University, Xi’an, ChinaSchool of Basic Medical Science, The Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, ChinaXi’an Key Laboratory of Pathogenic Microorganism and Tumor Immunity, Xi’an Medical University, Xi’an, ChinaSchool of Basic Medical Science, The Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, ChinaXi’an Key Laboratory of Pathogenic Microorganism and Tumor Immunity, Xi’an Medical University, Xi’an, ChinaDepartment of Mechanical and Nuclear Engineering, Kansas State University, Manhattan, KS, United StatesIntercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophisticated procedures. Microfluidics technique is considered as one of the powerful measures capable of precisely capturing and manipulating cells and achieving low reagent consumption and high throughput with decidedly integrated functional components. Over the past few years, microfluidics-based systems for intercellular interaction study at a single-cell level have become frequently adopted. This review focuses on microfluidic single-cell studies for intercellular interaction in a 2D or 3D environment with a variety of cell manipulating techniques and applications. The challenges to be overcome are highlighted.https://www.frontiersin.org/articles/10.3389/fcell.2021.680307/fullcell-cell communicationcell culturesingle-cell manipulationsingle-cell analysismicrofluidic technology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Long Pang Long Pang Jing Ding Xi-Xian Liu Zhixuan Kou Zhixuan Kou Lulu Guo Lulu Guo Xi Xu Xi Xu Shih-Kang Fan |
spellingShingle |
Long Pang Long Pang Jing Ding Xi-Xian Liu Zhixuan Kou Zhixuan Kou Lulu Guo Lulu Guo Xi Xu Xi Xu Shih-Kang Fan Microfluidics-Based Single-Cell Research for Intercellular Interaction Frontiers in Cell and Developmental Biology cell-cell communication cell culture single-cell manipulation single-cell analysis microfluidic technology |
author_facet |
Long Pang Long Pang Jing Ding Xi-Xian Liu Zhixuan Kou Zhixuan Kou Lulu Guo Lulu Guo Xi Xu Xi Xu Shih-Kang Fan |
author_sort |
Long Pang |
title |
Microfluidics-Based Single-Cell Research for Intercellular Interaction |
title_short |
Microfluidics-Based Single-Cell Research for Intercellular Interaction |
title_full |
Microfluidics-Based Single-Cell Research for Intercellular Interaction |
title_fullStr |
Microfluidics-Based Single-Cell Research for Intercellular Interaction |
title_full_unstemmed |
Microfluidics-Based Single-Cell Research for Intercellular Interaction |
title_sort |
microfluidics-based single-cell research for intercellular interaction |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-08-01 |
description |
Intercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophisticated procedures. Microfluidics technique is considered as one of the powerful measures capable of precisely capturing and manipulating cells and achieving low reagent consumption and high throughput with decidedly integrated functional components. Over the past few years, microfluidics-based systems for intercellular interaction study at a single-cell level have become frequently adopted. This review focuses on microfluidic single-cell studies for intercellular interaction in a 2D or 3D environment with a variety of cell manipulating techniques and applications. The challenges to be overcome are highlighted. |
topic |
cell-cell communication cell culture single-cell manipulation single-cell analysis microfluidic technology |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.680307/full |
work_keys_str_mv |
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