Microfluidics-Based Single-Cell Research for Intercellular Interaction

Intercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophis...

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Main Authors:	Long Pang, Jing Ding, Xi-Xian Liu, Zhixuan Kou, Lulu Guo, Xi Xu, Shih-Kang Fan
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-08-01
Series:	Frontiers in Cell and Developmental Biology
Subjects:	cell-cell communication cell culture single-cell manipulation single-cell analysis microfluidic technology
Online Access:	https://www.frontiersin.org/articles/10.3389/fcell.2021.680307/full

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spelling	doaj-78b9ba935aa9457b8e14db91720a02312021-08-12T09:23:11ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-08-01910.3389/fcell.2021.680307680307Microfluidics-Based Single-Cell Research for Intercellular InteractionLong Pang0Long Pang1Jing Ding2Xi-Xian Liu3Zhixuan Kou4Zhixuan Kou5Lulu Guo6Lulu Guo7Xi Xu8Xi Xu9Shih-Kang Fan10School of Basic Medical Science, The Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, ChinaXi’an Key Laboratory of Pathogenic Microorganism and Tumor Immunity, Xi’an Medical University, Xi’an, ChinaDepartment of Mechanical and Nuclear Engineering, Kansas State University, Manhattan, KS, United StatesKey Laboratory of Thermo-Fluid Science and Engineering of MOE, School of Energy and Power Engineering, Xi’an Jiaotong University, Xi’an, ChinaSchool of Basic Medical Science, The Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, ChinaXi’an Key Laboratory of Pathogenic Microorganism and Tumor Immunity, Xi’an Medical University, Xi’an, ChinaSchool of Basic Medical Science, The Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, ChinaXi’an Key Laboratory of Pathogenic Microorganism and Tumor Immunity, Xi’an Medical University, Xi’an, ChinaSchool of Basic Medical Science, The Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, ChinaXi’an Key Laboratory of Pathogenic Microorganism and Tumor Immunity, Xi’an Medical University, Xi’an, ChinaDepartment of Mechanical and Nuclear Engineering, Kansas State University, Manhattan, KS, United StatesIntercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophisticated procedures. Microfluidics technique is considered as one of the powerful measures capable of precisely capturing and manipulating cells and achieving low reagent consumption and high throughput with decidedly integrated functional components. Over the past few years, microfluidics-based systems for intercellular interaction study at a single-cell level have become frequently adopted. This review focuses on microfluidic single-cell studies for intercellular interaction in a 2D or 3D environment with a variety of cell manipulating techniques and applications. The challenges to be overcome are highlighted.https://www.frontiersin.org/articles/10.3389/fcell.2021.680307/fullcell-cell communicationcell culturesingle-cell manipulationsingle-cell analysismicrofluidic technology
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Long Pang Long Pang Jing Ding Xi-Xian Liu Zhixuan Kou Zhixuan Kou Lulu Guo Lulu Guo Xi Xu Xi Xu Shih-Kang Fan
spellingShingle	Long Pang Long Pang Jing Ding Xi-Xian Liu Zhixuan Kou Zhixuan Kou Lulu Guo Lulu Guo Xi Xu Xi Xu Shih-Kang Fan Microfluidics-Based Single-Cell Research for Intercellular Interaction Frontiers in Cell and Developmental Biology cell-cell communication cell culture single-cell manipulation single-cell analysis microfluidic technology
author_facet	Long Pang Long Pang Jing Ding Xi-Xian Liu Zhixuan Kou Zhixuan Kou Lulu Guo Lulu Guo Xi Xu Xi Xu Shih-Kang Fan
author_sort	Long Pang
title	Microfluidics-Based Single-Cell Research for Intercellular Interaction
title_short	Microfluidics-Based Single-Cell Research for Intercellular Interaction
title_full	Microfluidics-Based Single-Cell Research for Intercellular Interaction
title_fullStr	Microfluidics-Based Single-Cell Research for Intercellular Interaction
title_full_unstemmed	Microfluidics-Based Single-Cell Research for Intercellular Interaction
title_sort	microfluidics-based single-cell research for intercellular interaction
publisher	Frontiers Media S.A.
series	Frontiers in Cell and Developmental Biology
issn	2296-634X
publishDate	2021-08-01
description	Intercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophisticated procedures. Microfluidics technique is considered as one of the powerful measures capable of precisely capturing and manipulating cells and achieving low reagent consumption and high throughput with decidedly integrated functional components. Over the past few years, microfluidics-based systems for intercellular interaction study at a single-cell level have become frequently adopted. This review focuses on microfluidic single-cell studies for intercellular interaction in a 2D or 3D environment with a variety of cell manipulating techniques and applications. The challenges to be overcome are highlighted.
topic	cell-cell communication cell culture single-cell manipulation single-cell analysis microfluidic technology
url	https://www.frontiersin.org/articles/10.3389/fcell.2021.680307/full
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Microfluidics-Based Single-Cell Research for Intercellular Interaction

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