INFLIXIMAB AND TUMOUR NECROSIS FACTOR ALPHA MEASUREMENT ON INTESTINAL MUCOSA: A NEW TOOL FOR THE CLINIC?
Background: Infliximab (IFX) trough levels measurement could partially explain mechanisms of loss in response to this drug. However, little information exists on its concentration at the mucosal level or mucosal pharmacokinetics. Objective: The aim of this study was to investigate whether IFX cou...
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European Medical Journal
2016-12-01
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doaj-78c971ddbb0e43c4b4f35e0efa9e0edd2020-12-10T15:45:09ZengEuropean Medical JournalEuropean Medical Journal Gastroenterology2054-62032016-12-0151107115INFLIXIMAB AND TUMOUR NECROSIS FACTOR ALPHA MEASUREMENT ON INTESTINAL MUCOSA: A NEW TOOL FOR THE CLINIC?Silvia Pecere0Valentina Petito1Arianna Amato2Andrea Poscia3Alessandro Armuzzi4Loris Riccardo Lopetuso5Alessandro Sgambato6Giovanni Cammarota7Alfredo Papa8Antonio Gasbarrini9Franco Scaldaferri10Internal Medicine Department, Gastroenterology Division, Catholic University of the Sacred Heart Rome, Rome, ItalyInternal Medicine Department, Gastroenterology Division, Catholic University of the Sacred Heart Rome, Rome, ItalyInstitute of Anesthesia and Resuscitation, University of the Sacred Heart Rome, Rome, ItalyInstitute of Hygiene, Catholic University of the Sacred Heart Rome, Rome, ItalyInternal Medicine Department, Gastroenterology Division, Catholic University of the Sacred Heart Rome, Rome, ItalyInternal Medicine Department, Gastroenterology Division, Catholic University of the Sacred Heart Rome, Rome, ItalyInstitute of Pathology, Catholic University of the Sacred Heart Rome, Rome, ItalyInternal Medicine Department, Gastroenterology Division, Catholic University of the Sacred Heart Rome, Rome, ItalyInternal Medicine Department, Gastroenterology Division, Catholic University of the Sacred Heart Rome, Rome, ItalyInternal Medicine Department, Gastroenterology Division, Catholic University of the Sacred Heart Rome, Rome, ItalyInternal Medicine Department, Gastroenterology Division, Catholic University of the Sacred Heart Rome, Rome, ItalyBackground: Infliximab (IFX) trough levels measurement could partially explain mechanisms of loss in response to this drug. However, little information exists on its concentration at the mucosal level or mucosal pharmacokinetics. Objective: The aim of this study was to investigate whether IFX could be measured within intestinal mucosa, and whether a correlation between mucosal level, serum level, and clinical response could be hypothesised. Methods: Fifteen consecutive patients with inflammatory bowel disease receiving stable doses of IFX who underwent endoscopy were enrolled. Biopsies were taken from an affected and an unaffected area and cultured for 48 hours, and serum samples were also collected. IFX and tumour necrosis factor alpha (TNF-α) levels were measured using commercially available enzyme-linked immunosorbent assay kits. Results: IFX levels were detected in 80% of the colonic biopsy supernatants and in 60% of the serum samples. TNF-α intestinal mucosal levels were detectable in 100% of patients, while TNF-α serum levels were detectable in 75%. Mucosal and serum levels of IFX and TNF-α did not correlate; no correlation was found between the last infusion and serum or intestinal mucosal levels. Levels of IFX were more frequently undetectable in the mucosa of patients not responding to IFX therapy. Conclusions: Detectable levels of IFX and TNF-α can be found in intestinal mucosa. IFX mucosa levels could be useful to stratify patients into responders and non-responders to IXF therapy.https://www.emjreviews.com/gastroenterology/article/infliximab-and-tumour-necrosis-factor-alpha-measurement-on-intestinal-mucosa-a-new-tool-for-the-clinic/inflammatory bowel disease (ibd)trough levelsinfliximab (ifx) mucosal levelsifx serum levelstumour necrosis factor alpha (tnf-α)intestinal mucosa |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Silvia Pecere Valentina Petito Arianna Amato Andrea Poscia Alessandro Armuzzi Loris Riccardo Lopetuso Alessandro Sgambato Giovanni Cammarota Alfredo Papa Antonio Gasbarrini Franco Scaldaferri |
spellingShingle |
Silvia Pecere Valentina Petito Arianna Amato Andrea Poscia Alessandro Armuzzi Loris Riccardo Lopetuso Alessandro Sgambato Giovanni Cammarota Alfredo Papa Antonio Gasbarrini Franco Scaldaferri INFLIXIMAB AND TUMOUR NECROSIS FACTOR ALPHA MEASUREMENT ON INTESTINAL MUCOSA: A NEW TOOL FOR THE CLINIC? European Medical Journal Gastroenterology inflammatory bowel disease (ibd) trough levels infliximab (ifx) mucosal levels ifx serum levels tumour necrosis factor alpha (tnf-α) intestinal mucosa |
author_facet |
Silvia Pecere Valentina Petito Arianna Amato Andrea Poscia Alessandro Armuzzi Loris Riccardo Lopetuso Alessandro Sgambato Giovanni Cammarota Alfredo Papa Antonio Gasbarrini Franco Scaldaferri |
author_sort |
Silvia Pecere |
title |
INFLIXIMAB AND TUMOUR NECROSIS FACTOR ALPHA MEASUREMENT ON INTESTINAL MUCOSA: A NEW TOOL FOR THE CLINIC? |
title_short |
INFLIXIMAB AND TUMOUR NECROSIS FACTOR ALPHA MEASUREMENT ON INTESTINAL MUCOSA: A NEW TOOL FOR THE CLINIC? |
title_full |
INFLIXIMAB AND TUMOUR NECROSIS FACTOR ALPHA MEASUREMENT ON INTESTINAL MUCOSA: A NEW TOOL FOR THE CLINIC? |
title_fullStr |
INFLIXIMAB AND TUMOUR NECROSIS FACTOR ALPHA MEASUREMENT ON INTESTINAL MUCOSA: A NEW TOOL FOR THE CLINIC? |
title_full_unstemmed |
INFLIXIMAB AND TUMOUR NECROSIS FACTOR ALPHA MEASUREMENT ON INTESTINAL MUCOSA: A NEW TOOL FOR THE CLINIC? |
title_sort |
infliximab and tumour necrosis factor alpha measurement on intestinal mucosa: a new tool for the clinic? |
publisher |
European Medical Journal |
series |
European Medical Journal Gastroenterology |
issn |
2054-6203 |
publishDate |
2016-12-01 |
description |
Background: Infliximab (IFX) trough levels measurement could partially explain mechanisms of loss in response to this drug. However, little information exists on its concentration at the mucosal level or mucosal pharmacokinetics.
Objective: The aim of this study was to investigate whether IFX could be measured within intestinal mucosa, and whether a correlation between mucosal level, serum level, and clinical response could be hypothesised.
Methods: Fifteen consecutive patients with inflammatory bowel disease receiving stable doses of IFX who underwent endoscopy were enrolled. Biopsies were taken from an affected and an unaffected area and cultured for 48 hours, and serum samples were also collected. IFX and tumour necrosis factor alpha (TNF-α) levels were measured using commercially available enzyme-linked immunosorbent assay kits.
Results: IFX levels were detected in 80% of the colonic biopsy supernatants and in 60% of the serum samples. TNF-α intestinal mucosal levels were detectable in 100% of patients, while TNF-α serum levels were detectable in 75%. Mucosal and serum levels of IFX and TNF-α did not correlate; no correlation was found between the last infusion and serum or intestinal mucosal levels. Levels of IFX were more frequently undetectable in the mucosa of patients not responding to IFX therapy.
Conclusions: Detectable levels of IFX and TNF-α can be found in intestinal mucosa. IFX mucosa levels could be useful to stratify patients into responders and non-responders to IXF therapy. |
topic |
inflammatory bowel disease (ibd) trough levels infliximab (ifx) mucosal levels ifx serum levels tumour necrosis factor alpha (tnf-α) intestinal mucosa |
url |
https://www.emjreviews.com/gastroenterology/article/infliximab-and-tumour-necrosis-factor-alpha-measurement-on-intestinal-mucosa-a-new-tool-for-the-clinic/ |
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