Generation of IDUA knockout cell lines by CRISPR/Cas mediated genome editing
Background: The hurler syndrome is the most common form of lysosomal storage diseases (LSDs). In the present experiment, we intended to generate an IDUA targeting CRISPR-Cas system and deactivate the target gene using it. Materials and Methods: IUDA targeting sgRNA pair were cloned into the px335 pl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Lorestan University of Medical Science
2021-01-01
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| Series: | Yafteh |
| Subjects: | |
| Online Access: | http://yafte.lums.ac.ir/article-1-3103-en.html |