Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from <i>Sargassum aquifolium</i>
Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer. Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan. The determinat...
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doaj-78eee94cd53b48b3af4ece94341b244f2021-04-12T23:02:26ZengMDPI AGMarine Drugs1660-33972021-04-011921521510.3390/md19040215Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from <i>Sargassum aquifolium</i>Hui-Hua Hsiao0Tien-Chiu Wu1Yung-Hsiang Tsai2Chia-Hung Kuo3Ren-Han Huang4Yong-Han Hong5Chun-Yung Huang6Faculty of Medicine, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, TaiwanMackay Memorial Hospital Emergency Department, No. 92, Sec. 2, Zhongshan North Rd., Taipei City 10449, TaiwanDepartment of Nutrition, Yanchao Campus, I-Shou University, No. 8, Yida Rd., Jiaosu Village, Yanchao District, Kaohsiung City 82445, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, TaiwanIntensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer. Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan. The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging. In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2). SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses. The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome <i>c</i> release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades. Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway. Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer.https://www.mdpi.com/1660-3397/19/4/215anti-lung cancerapoptosisbrown algaefucoidanhuman lung carcinoma A-549 cellsoversulfation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui-Hua Hsiao Tien-Chiu Wu Yung-Hsiang Tsai Chia-Hung Kuo Ren-Han Huang Yong-Han Hong Chun-Yung Huang |
spellingShingle |
Hui-Hua Hsiao Tien-Chiu Wu Yung-Hsiang Tsai Chia-Hung Kuo Ren-Han Huang Yong-Han Hong Chun-Yung Huang Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from <i>Sargassum aquifolium</i> Marine Drugs anti-lung cancer apoptosis brown algae fucoidan human lung carcinoma A-549 cells oversulfation |
author_facet |
Hui-Hua Hsiao Tien-Chiu Wu Yung-Hsiang Tsai Chia-Hung Kuo Ren-Han Huang Yong-Han Hong Chun-Yung Huang |
author_sort |
Hui-Hua Hsiao |
title |
Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from <i>Sargassum aquifolium</i> |
title_short |
Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from <i>Sargassum aquifolium</i> |
title_full |
Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from <i>Sargassum aquifolium</i> |
title_fullStr |
Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from <i>Sargassum aquifolium</i> |
title_full_unstemmed |
Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from <i>Sargassum aquifolium</i> |
title_sort |
effect of oversulfation on the composition, structure, and in vitro anti-lung cancer activity of fucoidans extracted from <i>sargassum aquifolium</i> |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2021-04-01 |
description |
Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer. Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan. The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging. In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2). SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses. The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome <i>c</i> release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades. Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway. Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer. |
topic |
anti-lung cancer apoptosis brown algae fucoidan human lung carcinoma A-549 cells oversulfation |
url |
https://www.mdpi.com/1660-3397/19/4/215 |
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