Comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in Pueraria thomsonii Benth.
Pueraria thomsonii Benth is an important medicinal plant. Transcriptome sequencing, unigene assembly, the annotation of transcripts and the study of gene expression profiles play vital roles in gene function research. However, the full-length transcriptome of P. thomsonii remains unknown. Here, we o...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0217593 |
id |
doaj-790cdd72dcdc4f9493f390a1fa1871ce |
---|---|
record_format |
Article |
spelling |
doaj-790cdd72dcdc4f9493f390a1fa1871ce2021-03-03T20:38:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01146e021759310.1371/journal.pone.0217593Comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in Pueraria thomsonii Benth.Meijun HeYiwei YaoYanni LiMeng YangYu LiBin WuDazhao YuPueraria thomsonii Benth is an important medicinal plant. Transcriptome sequencing, unigene assembly, the annotation of transcripts and the study of gene expression profiles play vital roles in gene function research. However, the full-length transcriptome of P. thomsonii remains unknown. Here, we obtained 44,339 nonredundant transcripts of P. thomsonii by using the PacBio RS II Isoform and Illumina sequencing platforms, of which 43,195 were annotated genes. Compared with the expression levels in the plant roots, those of transcripts with a |fold change| ≥ 4 and FDR < 0.01 in the leaves or stems were assigned as differentially expressed transcripts (DETs). In total, we found 9,225 DETs, 32 of which came from structural genes that were potentially involved in isoflavone biosynthesis. The expression profiles of 8 structural genes from the RNA-Seq data were validated by qRT-PCR. We identified 437 transcription factors (TFs) that were positively or negatively correlated with at least 1 of the structural genes involved in isoflavone biosynthesis using Pearson correlation coefficients (r) (r > 0.8 or r < -0.8). We also identified a total of 32 microRNAs (miRNAs), which targeted 805 transcripts. These miRNAs caused enriched function in 'ATP binding', 'defense response', 'ADP binding', and 'signal transduction'. Interestingly, MIR156a potentially promoted isoflavone biosynthesis by repressing SBP, and MIR319 promoted isoflavone biosynthesis by repressing TCP and HB-HD-ZIP. Finally, we identified 2,690 alternative splicing events, including that of the structural genes of trans-cinnamate 4-monooxygenase and pullulanase, which are potentially involved in the biosynthesis of isoflavone and starch, respectively, and of three TFs potentially involved in isoflavone biosynthesis. Together, these results provide us with comprehensive insight into the gene expression and regulation of P. thomsonii.https://doi.org/10.1371/journal.pone.0217593 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meijun He Yiwei Yao Yanni Li Meng Yang Yu Li Bin Wu Dazhao Yu |
spellingShingle |
Meijun He Yiwei Yao Yanni Li Meng Yang Yu Li Bin Wu Dazhao Yu Comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in Pueraria thomsonii Benth. PLoS ONE |
author_facet |
Meijun He Yiwei Yao Yanni Li Meng Yang Yu Li Bin Wu Dazhao Yu |
author_sort |
Meijun He |
title |
Comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in Pueraria thomsonii Benth. |
title_short |
Comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in Pueraria thomsonii Benth. |
title_full |
Comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in Pueraria thomsonii Benth. |
title_fullStr |
Comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in Pueraria thomsonii Benth. |
title_full_unstemmed |
Comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in Pueraria thomsonii Benth. |
title_sort |
comprehensive transcriptome analysis reveals genes potentially involved in isoflavone biosynthesis in pueraria thomsonii benth. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Pueraria thomsonii Benth is an important medicinal plant. Transcriptome sequencing, unigene assembly, the annotation of transcripts and the study of gene expression profiles play vital roles in gene function research. However, the full-length transcriptome of P. thomsonii remains unknown. Here, we obtained 44,339 nonredundant transcripts of P. thomsonii by using the PacBio RS II Isoform and Illumina sequencing platforms, of which 43,195 were annotated genes. Compared with the expression levels in the plant roots, those of transcripts with a |fold change| ≥ 4 and FDR < 0.01 in the leaves or stems were assigned as differentially expressed transcripts (DETs). In total, we found 9,225 DETs, 32 of which came from structural genes that were potentially involved in isoflavone biosynthesis. The expression profiles of 8 structural genes from the RNA-Seq data were validated by qRT-PCR. We identified 437 transcription factors (TFs) that were positively or negatively correlated with at least 1 of the structural genes involved in isoflavone biosynthesis using Pearson correlation coefficients (r) (r > 0.8 or r < -0.8). We also identified a total of 32 microRNAs (miRNAs), which targeted 805 transcripts. These miRNAs caused enriched function in 'ATP binding', 'defense response', 'ADP binding', and 'signal transduction'. Interestingly, MIR156a potentially promoted isoflavone biosynthesis by repressing SBP, and MIR319 promoted isoflavone biosynthesis by repressing TCP and HB-HD-ZIP. Finally, we identified 2,690 alternative splicing events, including that of the structural genes of trans-cinnamate 4-monooxygenase and pullulanase, which are potentially involved in the biosynthesis of isoflavone and starch, respectively, and of three TFs potentially involved in isoflavone biosynthesis. Together, these results provide us with comprehensive insight into the gene expression and regulation of P. thomsonii. |
url |
https://doi.org/10.1371/journal.pone.0217593 |
work_keys_str_mv |
AT meijunhe comprehensivetranscriptomeanalysisrevealsgenespotentiallyinvolvedinisoflavonebiosynthesisinpuerariathomsoniibenth AT yiweiyao comprehensivetranscriptomeanalysisrevealsgenespotentiallyinvolvedinisoflavonebiosynthesisinpuerariathomsoniibenth AT yannili comprehensivetranscriptomeanalysisrevealsgenespotentiallyinvolvedinisoflavonebiosynthesisinpuerariathomsoniibenth AT mengyang comprehensivetranscriptomeanalysisrevealsgenespotentiallyinvolvedinisoflavonebiosynthesisinpuerariathomsoniibenth AT yuli comprehensivetranscriptomeanalysisrevealsgenespotentiallyinvolvedinisoflavonebiosynthesisinpuerariathomsoniibenth AT binwu comprehensivetranscriptomeanalysisrevealsgenespotentiallyinvolvedinisoflavonebiosynthesisinpuerariathomsoniibenth AT dazhaoyu comprehensivetranscriptomeanalysisrevealsgenespotentiallyinvolvedinisoflavonebiosynthesisinpuerariathomsoniibenth |
_version_ |
1714821409583136768 |