Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis
Advances in biotechnology have increased the demand for suitable analytical techniques for the analysis of genetically modified organisms. Study of the substantial equivalence, discrimination between transgenic and non-transgenic cultivars, study of the unintended effects caused by a genetic modifi...
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doaj-793131fe06a242778fe5151cae1f5fb02020-11-24T22:50:04ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-12-011512238512387710.3390/ijms151223851ijms151223851Characterization and Study of Transgenic Cultivars by Capillary and Microchip ElectrophoresisElena Domínguez Vega0Maria Luisa Marina1Division of BioAnalytical Chemistry, AIMMS Research Group of BioMolecular Analysis, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The NetherlandsDepartment of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, SpainAdvances in biotechnology have increased the demand for suitable analytical techniques for the analysis of genetically modified organisms. Study of the substantial equivalence, discrimination between transgenic and non-transgenic cultivars, study of the unintended effects caused by a genetic modification or their response to diverse situations or stress conditions (e.g., environmental, climatic, infections) are some of the concerns that need to be addressed. Capillary electrophoresis (CE) is emerging as an alternative to conventional techniques for the study and characterization of genetically modified organisms. This article reviews the most recent applications of CE for the analysis and characterization of transgenic cultivars in the last five years. Different strategies have been described depending on the level analyzed (DNA, proteins or metabolites). Capillary gel electrophoresis (CGE) has shown to be particularly useful for the analysis of DNA fragments amplified by PCR. Metabolites and proteins have been mainly separated using capillary zone electrophoresis (CZE) using UV and MS detection. Electrophoretic chips have also proven their ability in the analysis of transgenic cultivars and a section describing the new applications is also included.http://www.mdpi.com/1422-0067/15/12/23851capillary electrophoresismicrochip electrophoresistransgenic cultivarsGMOsafety assessmentfood |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elena Domínguez Vega Maria Luisa Marina |
spellingShingle |
Elena Domínguez Vega Maria Luisa Marina Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis International Journal of Molecular Sciences capillary electrophoresis microchip electrophoresis transgenic cultivars GMO safety assessment food |
author_facet |
Elena Domínguez Vega Maria Luisa Marina |
author_sort |
Elena Domínguez Vega |
title |
Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis |
title_short |
Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis |
title_full |
Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis |
title_fullStr |
Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis |
title_full_unstemmed |
Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis |
title_sort |
characterization and study of transgenic cultivars by capillary and microchip electrophoresis |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2014-12-01 |
description |
Advances in biotechnology have increased the demand for suitable analytical techniques for the analysis of genetically modified organisms. Study of the substantial equivalence, discrimination between transgenic and non-transgenic cultivars, study of the unintended effects caused by a genetic modification or their response to diverse situations or stress conditions (e.g., environmental, climatic, infections) are some of the concerns that need to be addressed. Capillary electrophoresis (CE) is emerging as an alternative to conventional techniques for the study and characterization of genetically modified organisms. This article reviews the most recent applications of CE for the analysis and characterization of transgenic cultivars in the last five years. Different strategies have been described depending on the level analyzed (DNA, proteins or metabolites). Capillary gel electrophoresis (CGE) has shown to be particularly useful for the analysis of DNA fragments amplified by PCR. Metabolites and proteins have been mainly separated using capillary zone electrophoresis (CZE) using UV and MS detection. Electrophoretic chips have also proven their ability in the analysis of transgenic cultivars and a section describing the new applications is also included. |
topic |
capillary electrophoresis microchip electrophoresis transgenic cultivars GMO safety assessment food |
url |
http://www.mdpi.com/1422-0067/15/12/23851 |
work_keys_str_mv |
AT elenadominguezvega characterizationandstudyoftransgeniccultivarsbycapillaryandmicrochipelectrophoresis AT marialuisamarina characterizationandstudyoftransgeniccultivarsbycapillaryandmicrochipelectrophoresis |
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