Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses
Background: In this work, the xylanase production by Penicillium chrysogenum F-15 strain was investigated using agroindustrial biomass as substrate. The xylanase was purified, characterized and applied in hemicellulose hydrolysis. Results: The highest xylanase production was obtained when cultivatio...
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doaj-793c5078d87f4d60ad9276c2300a91d72020-11-25T00:05:06ZengElsevierElectronic Journal of Biotechnology0717-34582018-05-01333945Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicellulosesCárol Cabral Terrone0Caroline de Freitas1César Rafael Fanchini Terrasan2Alex Fernando de Almeida3Eleonora Cano Carmona4Departamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP, Av. 24A, 1515, Bela Vista, 13506-900, Rio Claro, SP, BrazilDepartamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP, Av. 24A, 1515, Bela Vista, 13506-900, Rio Claro, SP, BrazilDepartamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP, Av. 24A, 1515, Bela Vista, 13506-900, Rio Claro, SP, BrazilDepartamento de Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Tocantins - UFT, Rua Badejós, Chácaras 69/72, Zona Rural, 77402-970, Gurupi, TO, BrazilDepartamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP, Av. 24A, 1515, Bela Vista, 13506-900, Rio Claro, SP, Brazil; Corresponding author.Background: In this work, the xylanase production by Penicillium chrysogenum F-15 strain was investigated using agroindustrial biomass as substrate. The xylanase was purified, characterized and applied in hemicellulose hydrolysis. Results: The highest xylanase production was obtained when cultivation was carried out with sugar cane bagasse as carbon source, at pH 6.0 and 20°C, under static condition for 8 d. The enzyme was purified by a sequence of ion exchange and size exclusion chromatography, presenting final specific activity of 834.2 U·mg·prot-1. The molecular mass of the purified enzyme estimated by SDS-PAGE was 22.1 kDa. The optimum activity was at pH 6.5 and 45°C. The enzyme was stable at 40°C with half-life of 35 min, and in the pH range from 4.5 to 10.0. The activity was increased in the presence of Mg+2 and Mn+2 and reducing agents such as DTT and β-mercaptoethanol, but it was reduced by Cu+2 and Pb+2. The xylanase presented Km of 2.3 mM and Vmax of 731.8 U·mg·prot-1 with birchwood xylan as substrate. This xylanase presented differences in its properties when it was compared to the xylanases from other P. chrysogenum strains. Conclusion: The xylanase from P. chrysogenum F-15 showed lower enzymatic activity on commercial xylan than on hemicellulose from agroindustry biomass and its biochemistry characteristics, such as stability at 40°C and pH from 4.0 to 10.0, shows the potential of this enzyme for application in food, feed, pulp and paper industries and for bioethanol production.How to cite: Terrone CC, Freitas C, Terrasan CRF, et al. Agroindustrial biomass for xylanase production by Penicillium chrysogenum: purification, biochemical properties and hydrolysis of hemicelluloses. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.04.001. Keywords: Agroindustrial biomass, Enzyme production, Hemicellulose hydrolysis, Hemicelluloses, Penicillium chrysogenum, pH stability, Sugar cane bagasse, Thermal stability, Xylanase production, Xylanolytic enzymehttp://www.sciencedirect.com/science/article/pii/S0717345818300113 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cárol Cabral Terrone Caroline de Freitas César Rafael Fanchini Terrasan Alex Fernando de Almeida Eleonora Cano Carmona |
spellingShingle |
Cárol Cabral Terrone Caroline de Freitas César Rafael Fanchini Terrasan Alex Fernando de Almeida Eleonora Cano Carmona Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses Electronic Journal of Biotechnology |
author_facet |
Cárol Cabral Terrone Caroline de Freitas César Rafael Fanchini Terrasan Alex Fernando de Almeida Eleonora Cano Carmona |
author_sort |
Cárol Cabral Terrone |
title |
Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses |
title_short |
Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses |
title_full |
Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses |
title_fullStr |
Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses |
title_full_unstemmed |
Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses |
title_sort |
agroindustrial biomass for xylanase production by penicillium chrysogenum: purification, biochemical properties and hydrolysis of hemicelluloses |
publisher |
Elsevier |
series |
Electronic Journal of Biotechnology |
issn |
0717-3458 |
publishDate |
2018-05-01 |
description |
Background: In this work, the xylanase production by Penicillium chrysogenum F-15 strain was investigated using agroindustrial biomass as substrate. The xylanase was purified, characterized and applied in hemicellulose hydrolysis. Results: The highest xylanase production was obtained when cultivation was carried out with sugar cane bagasse as carbon source, at pH 6.0 and 20°C, under static condition for 8 d. The enzyme was purified by a sequence of ion exchange and size exclusion chromatography, presenting final specific activity of 834.2 U·mg·prot-1. The molecular mass of the purified enzyme estimated by SDS-PAGE was 22.1 kDa. The optimum activity was at pH 6.5 and 45°C. The enzyme was stable at 40°C with half-life of 35 min, and in the pH range from 4.5 to 10.0. The activity was increased in the presence of Mg+2 and Mn+2 and reducing agents such as DTT and β-mercaptoethanol, but it was reduced by Cu+2 and Pb+2. The xylanase presented Km of 2.3 mM and Vmax of 731.8 U·mg·prot-1 with birchwood xylan as substrate. This xylanase presented differences in its properties when it was compared to the xylanases from other P. chrysogenum strains. Conclusion: The xylanase from P. chrysogenum F-15 showed lower enzymatic activity on commercial xylan than on hemicellulose from agroindustry biomass and its biochemistry characteristics, such as stability at 40°C and pH from 4.0 to 10.0, shows the potential of this enzyme for application in food, feed, pulp and paper industries and for bioethanol production.How to cite: Terrone CC, Freitas C, Terrasan CRF, et al. Agroindustrial biomass for xylanase production by Penicillium chrysogenum: purification, biochemical properties and hydrolysis of hemicelluloses. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.04.001. Keywords: Agroindustrial biomass, Enzyme production, Hemicellulose hydrolysis, Hemicelluloses, Penicillium chrysogenum, pH stability, Sugar cane bagasse, Thermal stability, Xylanase production, Xylanolytic enzyme |
url |
http://www.sciencedirect.com/science/article/pii/S0717345818300113 |
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