Multipotent PDGFRβ-expressing cells in the circulation of stroke patients
Tissue pericytes respond to injury, and support vascular and tissue regeneration. The presence of pericytes in the circulation may provide an attractive framework for tissue regeneration. Here, we detected multipotent pericyte-like cells in the circulating blood and determined its profiles during ce...
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doaj-793e777764dd4403af5702f89933f41a2021-03-22T12:36:08ZengElsevierNeurobiology of Disease1095-953X2011-02-01412489497Multipotent PDGFRβ-expressing cells in the circulation of stroke patientsKeun-Hwa Jung0Kon Chu1Soon-Tae Lee2Jae-Jun Bahn3Daejong Jeon4Jin-Hee Kim5Soyun Kim6Chong-Hyun Won7Manho Kim8Sang Kun Lee9Jae-Kyu Roh10Department of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South KoreaDepartment of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South KoreaDepartment of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South KoreaDepartment of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South KoreaDepartment of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of KoreaDepartment of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South KoreaDepartment of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South KoreaDepartment of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South KoreaDepartment of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South KoreaDepartment of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South KoreaDepartment of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South Korea; Corresponding author. Department of Neurology, Seoul National University Hospital, 28,Yongon-Dong, Chongro-Gu, Seoul, 110-744, Republic of Korea.Tissue pericytes respond to injury, and support vascular and tissue regeneration. The presence of pericytes in the circulation may provide an attractive framework for tissue regeneration. Here, we detected multipotent pericyte-like cells in the circulating blood and determined its profiles during cerebral ischemia. Pericyte-like cells were isolated from the peripheral blood of acute stroke patients or asymptomatic individuals with vascular risk factors by fluorescence or magnetic activated cell sorting with anti-PDGF receptor-beta (PDGFRβ) antibody. The morphologic and molecular features of circulating PDGFRβ+ cells were compared with tissue pericytes, and the associations with respect to quantity in the blood, culture outcome, and patient characteristics were analyzed. We found an increase in circulating PDGFRβ+ cells in acute stroke patients compared to controls and a correlation with neurologic impairment. The isolated PDGFRβ+ cells expressed mesenchymal stem cell markers, proliferated, and were multipotent under permissive culture conditions. The multipotent nature of these cells was comparable to fat-derived PDGFRβ+ cells. These cells could be obtained by pharmacologic stimulation using bone marrow mobilizer. Circulating PDGFRβ+ cells will be useful for future research involving endogenous recovery or autologous cell-based therapy.http://www.sciencedirect.com/science/article/pii/S0969996110003633PDGF receptor-betaPericytePeripheral bloodStrokeRecovery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keun-Hwa Jung Kon Chu Soon-Tae Lee Jae-Jun Bahn Daejong Jeon Jin-Hee Kim Soyun Kim Chong-Hyun Won Manho Kim Sang Kun Lee Jae-Kyu Roh |
spellingShingle |
Keun-Hwa Jung Kon Chu Soon-Tae Lee Jae-Jun Bahn Daejong Jeon Jin-Hee Kim Soyun Kim Chong-Hyun Won Manho Kim Sang Kun Lee Jae-Kyu Roh Multipotent PDGFRβ-expressing cells in the circulation of stroke patients Neurobiology of Disease PDGF receptor-beta Pericyte Peripheral blood Stroke Recovery |
author_facet |
Keun-Hwa Jung Kon Chu Soon-Tae Lee Jae-Jun Bahn Daejong Jeon Jin-Hee Kim Soyun Kim Chong-Hyun Won Manho Kim Sang Kun Lee Jae-Kyu Roh |
author_sort |
Keun-Hwa Jung |
title |
Multipotent PDGFRβ-expressing cells in the circulation of stroke patients |
title_short |
Multipotent PDGFRβ-expressing cells in the circulation of stroke patients |
title_full |
Multipotent PDGFRβ-expressing cells in the circulation of stroke patients |
title_fullStr |
Multipotent PDGFRβ-expressing cells in the circulation of stroke patients |
title_full_unstemmed |
Multipotent PDGFRβ-expressing cells in the circulation of stroke patients |
title_sort |
multipotent pdgfrβ-expressing cells in the circulation of stroke patients |
publisher |
Elsevier |
series |
Neurobiology of Disease |
issn |
1095-953X |
publishDate |
2011-02-01 |
description |
Tissue pericytes respond to injury, and support vascular and tissue regeneration. The presence of pericytes in the circulation may provide an attractive framework for tissue regeneration. Here, we detected multipotent pericyte-like cells in the circulating blood and determined its profiles during cerebral ischemia. Pericyte-like cells were isolated from the peripheral blood of acute stroke patients or asymptomatic individuals with vascular risk factors by fluorescence or magnetic activated cell sorting with anti-PDGF receptor-beta (PDGFRβ) antibody. The morphologic and molecular features of circulating PDGFRβ+ cells were compared with tissue pericytes, and the associations with respect to quantity in the blood, culture outcome, and patient characteristics were analyzed. We found an increase in circulating PDGFRβ+ cells in acute stroke patients compared to controls and a correlation with neurologic impairment. The isolated PDGFRβ+ cells expressed mesenchymal stem cell markers, proliferated, and were multipotent under permissive culture conditions. The multipotent nature of these cells was comparable to fat-derived PDGFRβ+ cells. These cells could be obtained by pharmacologic stimulation using bone marrow mobilizer. Circulating PDGFRβ+ cells will be useful for future research involving endogenous recovery or autologous cell-based therapy. |
topic |
PDGF receptor-beta Pericyte Peripheral blood Stroke Recovery |
url |
http://www.sciencedirect.com/science/article/pii/S0969996110003633 |
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