Differentiating Cerebellar Impact on Thalamic Nuclei
The cerebellum plays a role in coordination of movements and non-motor functions. Cerebellar nuclei (CN) axons connect to various parts of the thalamo-cortical network, but detailed information on the characteristics of cerebello-thalamic connections is lacking. Here, we assessed the cerebellar inpu...
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doaj-7958ff97741b40bd9eafa8a7509b0b4a2020-11-25T01:30:16ZengElsevierCell Reports2211-12472018-05-012392690270410.1016/j.celrep.2018.04.098Differentiating Cerebellar Impact on Thalamic NucleiSimona V. Gornati0Carmen B. Schäfer1Oscar H.J. Eelkman Rooda2Alex L. Nigg3Chris I. De Zeeuw4Freek E. Hoebeek5Department of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsDepartment of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsDepartment of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsDepartment of Pathology, Optical Imaging Center, Erasmus MC, 3015 AA Rotterdam, The NetherlandsDepartment of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsDepartment of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsThe cerebellum plays a role in coordination of movements and non-motor functions. Cerebellar nuclei (CN) axons connect to various parts of the thalamo-cortical network, but detailed information on the characteristics of cerebello-thalamic connections is lacking. Here, we assessed the cerebellar input to the ventrolateral (VL), ventromedial (VM), and centrolateral (CL) thalamus. Confocal and electron microscopy showed an increased density and size of CN axon terminals in VL compared to VM or CL. Electrophysiological recordings in vitro revealed that optogenetic CN stimulation resulted in enhanced charge transfer and action potential firing in VL neurons compared to VM or CL neurons, despite that the paired-pulse ratio was not significantly different. Together, these findings indicate that the impact of CN input onto neurons of different thalamic nuclei varies substantially, which highlights the possibility that cerebellar output differentially controls various parts of the thalamo-cortical network.http://www.sciencedirect.com/science/article/pii/S2211124718306764optogeneticsthalamuscerebellar nucleimorphologysynaptic transmission |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simona V. Gornati Carmen B. Schäfer Oscar H.J. Eelkman Rooda Alex L. Nigg Chris I. De Zeeuw Freek E. Hoebeek |
spellingShingle |
Simona V. Gornati Carmen B. Schäfer Oscar H.J. Eelkman Rooda Alex L. Nigg Chris I. De Zeeuw Freek E. Hoebeek Differentiating Cerebellar Impact on Thalamic Nuclei Cell Reports optogenetics thalamus cerebellar nuclei morphology synaptic transmission |
author_facet |
Simona V. Gornati Carmen B. Schäfer Oscar H.J. Eelkman Rooda Alex L. Nigg Chris I. De Zeeuw Freek E. Hoebeek |
author_sort |
Simona V. Gornati |
title |
Differentiating Cerebellar Impact on Thalamic Nuclei |
title_short |
Differentiating Cerebellar Impact on Thalamic Nuclei |
title_full |
Differentiating Cerebellar Impact on Thalamic Nuclei |
title_fullStr |
Differentiating Cerebellar Impact on Thalamic Nuclei |
title_full_unstemmed |
Differentiating Cerebellar Impact on Thalamic Nuclei |
title_sort |
differentiating cerebellar impact on thalamic nuclei |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2018-05-01 |
description |
The cerebellum plays a role in coordination of movements and non-motor functions. Cerebellar nuclei (CN) axons connect to various parts of the thalamo-cortical network, but detailed information on the characteristics of cerebello-thalamic connections is lacking. Here, we assessed the cerebellar input to the ventrolateral (VL), ventromedial (VM), and centrolateral (CL) thalamus. Confocal and electron microscopy showed an increased density and size of CN axon terminals in VL compared to VM or CL. Electrophysiological recordings in vitro revealed that optogenetic CN stimulation resulted in enhanced charge transfer and action potential firing in VL neurons compared to VM or CL neurons, despite that the paired-pulse ratio was not significantly different. Together, these findings indicate that the impact of CN input onto neurons of different thalamic nuclei varies substantially, which highlights the possibility that cerebellar output differentially controls various parts of the thalamo-cortical network. |
topic |
optogenetics thalamus cerebellar nuclei morphology synaptic transmission |
url |
http://www.sciencedirect.com/science/article/pii/S2211124718306764 |
work_keys_str_mv |
AT simonavgornati differentiatingcerebellarimpactonthalamicnuclei AT carmenbschafer differentiatingcerebellarimpactonthalamicnuclei AT oscarhjeelkmanrooda differentiatingcerebellarimpactonthalamicnuclei AT alexlnigg differentiatingcerebellarimpactonthalamicnuclei AT chrisidezeeuw differentiatingcerebellarimpactonthalamicnuclei AT freekehoebeek differentiatingcerebellarimpactonthalamicnuclei |
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