Differentiating Cerebellar Impact on Thalamic Nuclei

The cerebellum plays a role in coordination of movements and non-motor functions. Cerebellar nuclei (CN) axons connect to various parts of the thalamo-cortical network, but detailed information on the characteristics of cerebello-thalamic connections is lacking. Here, we assessed the cerebellar inpu...

Full description

Bibliographic Details
Main Authors: Simona V. Gornati, Carmen B. Schäfer, Oscar H.J. Eelkman Rooda, Alex L. Nigg, Chris I. De Zeeuw, Freek E. Hoebeek
Format: Article
Language:English
Published: Elsevier 2018-05-01
Series:Cell Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124718306764
id doaj-7958ff97741b40bd9eafa8a7509b0b4a
record_format Article
spelling doaj-7958ff97741b40bd9eafa8a7509b0b4a2020-11-25T01:30:16ZengElsevierCell Reports2211-12472018-05-012392690270410.1016/j.celrep.2018.04.098Differentiating Cerebellar Impact on Thalamic NucleiSimona V. Gornati0Carmen B. Schäfer1Oscar H.J. Eelkman Rooda2Alex L. Nigg3Chris I. De Zeeuw4Freek E. Hoebeek5Department of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsDepartment of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsDepartment of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsDepartment of Pathology, Optical Imaging Center, Erasmus MC, 3015 AA Rotterdam, The NetherlandsDepartment of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsDepartment of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The NetherlandsThe cerebellum plays a role in coordination of movements and non-motor functions. Cerebellar nuclei (CN) axons connect to various parts of the thalamo-cortical network, but detailed information on the characteristics of cerebello-thalamic connections is lacking. Here, we assessed the cerebellar input to the ventrolateral (VL), ventromedial (VM), and centrolateral (CL) thalamus. Confocal and electron microscopy showed an increased density and size of CN axon terminals in VL compared to VM or CL. Electrophysiological recordings in vitro revealed that optogenetic CN stimulation resulted in enhanced charge transfer and action potential firing in VL neurons compared to VM or CL neurons, despite that the paired-pulse ratio was not significantly different. Together, these findings indicate that the impact of CN input onto neurons of different thalamic nuclei varies substantially, which highlights the possibility that cerebellar output differentially controls various parts of the thalamo-cortical network.http://www.sciencedirect.com/science/article/pii/S2211124718306764optogeneticsthalamuscerebellar nucleimorphologysynaptic transmission
collection DOAJ
language English
format Article
sources DOAJ
author Simona V. Gornati
Carmen B. Schäfer
Oscar H.J. Eelkman Rooda
Alex L. Nigg
Chris I. De Zeeuw
Freek E. Hoebeek
spellingShingle Simona V. Gornati
Carmen B. Schäfer
Oscar H.J. Eelkman Rooda
Alex L. Nigg
Chris I. De Zeeuw
Freek E. Hoebeek
Differentiating Cerebellar Impact on Thalamic Nuclei
Cell Reports
optogenetics
thalamus
cerebellar nuclei
morphology
synaptic transmission
author_facet Simona V. Gornati
Carmen B. Schäfer
Oscar H.J. Eelkman Rooda
Alex L. Nigg
Chris I. De Zeeuw
Freek E. Hoebeek
author_sort Simona V. Gornati
title Differentiating Cerebellar Impact on Thalamic Nuclei
title_short Differentiating Cerebellar Impact on Thalamic Nuclei
title_full Differentiating Cerebellar Impact on Thalamic Nuclei
title_fullStr Differentiating Cerebellar Impact on Thalamic Nuclei
title_full_unstemmed Differentiating Cerebellar Impact on Thalamic Nuclei
title_sort differentiating cerebellar impact on thalamic nuclei
publisher Elsevier
series Cell Reports
issn 2211-1247
publishDate 2018-05-01
description The cerebellum plays a role in coordination of movements and non-motor functions. Cerebellar nuclei (CN) axons connect to various parts of the thalamo-cortical network, but detailed information on the characteristics of cerebello-thalamic connections is lacking. Here, we assessed the cerebellar input to the ventrolateral (VL), ventromedial (VM), and centrolateral (CL) thalamus. Confocal and electron microscopy showed an increased density and size of CN axon terminals in VL compared to VM or CL. Electrophysiological recordings in vitro revealed that optogenetic CN stimulation resulted in enhanced charge transfer and action potential firing in VL neurons compared to VM or CL neurons, despite that the paired-pulse ratio was not significantly different. Together, these findings indicate that the impact of CN input onto neurons of different thalamic nuclei varies substantially, which highlights the possibility that cerebellar output differentially controls various parts of the thalamo-cortical network.
topic optogenetics
thalamus
cerebellar nuclei
morphology
synaptic transmission
url http://www.sciencedirect.com/science/article/pii/S2211124718306764
work_keys_str_mv AT simonavgornati differentiatingcerebellarimpactonthalamicnuclei
AT carmenbschafer differentiatingcerebellarimpactonthalamicnuclei
AT oscarhjeelkmanrooda differentiatingcerebellarimpactonthalamicnuclei
AT alexlnigg differentiatingcerebellarimpactonthalamicnuclei
AT chrisidezeeuw differentiatingcerebellarimpactonthalamicnuclei
AT freekehoebeek differentiatingcerebellarimpactonthalamicnuclei
_version_ 1725092451083878400