Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment.
<h4>Background</h4>In view of the potential immunosuppressive and regenerative properties of mesenchymal stem cells (MSC), we investigated whether transplantation of adipose tissue-derived stem cells (ASC) could be used to control the granulomatous reaction in the liver of mice infected...
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doaj-7959d0ab9b584983ba53552645a1411f2021-03-03T08:25:03ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352020-08-01148e000863510.1371/journal.pntd.0008635Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment.Vitor Hugo Simões MirandaTalita Rocha GomesDirli Emerick EllerLorena de Cássia Neres FerrazAna Thereza ChavesKelly Alves BicalhoCarlos Eduardo Calzavara SilvaAlexander BirbrairMarcelo Antônio Pascoal XavierAlfredo Miranda de GoesRodrigo Corrêa-OliveiraÉrica Alessandra Rocha AlvesAdriana Bozzi<h4>Background</h4>In view of the potential immunosuppressive and regenerative properties of mesenchymal stem cells (MSC), we investigated whether transplantation of adipose tissue-derived stem cells (ASC) could be used to control the granulomatous reaction in the liver of mice infected with Schistosoma mansoni after Praziquantel (PZQ) treatment.<h4>Methodology/prinicpal findings</h4>C57BL/6 mice infected with S. mansoni were treated with PZQ and transplanted intravenously with ASC from uninfected mice. Liver morpho-physiological and immunological analyses were performed. The combined PZQ/ASC therapy significantly reduced the volume of hepatic granulomas, as well as liver damage as measured by ALT levels. We also observed that ASC accelerated the progression of the granulomatous inflammation to the advanced/curative phase. The faster healing interfered with the expression of CD28 and CTLA-4 molecules in CD4+ T lymphocytes, and the levels of IL-10 and IL-17 cytokines, mainly in the livers of PZQ/ASC-treated mice.<h4>Conclusions</h4>Our results show that ASC therapy after PZQ treatment results in smaller granulomas with little tissue damage, suggesting the potential of ASC for the development of novel therapeutic approaches to minimize hepatic lesions as well as a granulomatous reaction following S. mansoni infection. Further studies using the chronic model of schistosomiasis are required to corroborate the therapeutic use of ASC for schistosomiasis.https://doi.org/10.1371/journal.pntd.0008635 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vitor Hugo Simões Miranda Talita Rocha Gomes Dirli Emerick Eller Lorena de Cássia Neres Ferraz Ana Thereza Chaves Kelly Alves Bicalho Carlos Eduardo Calzavara Silva Alexander Birbrair Marcelo Antônio Pascoal Xavier Alfredo Miranda de Goes Rodrigo Corrêa-Oliveira Érica Alessandra Rocha Alves Adriana Bozzi |
spellingShingle |
Vitor Hugo Simões Miranda Talita Rocha Gomes Dirli Emerick Eller Lorena de Cássia Neres Ferraz Ana Thereza Chaves Kelly Alves Bicalho Carlos Eduardo Calzavara Silva Alexander Birbrair Marcelo Antônio Pascoal Xavier Alfredo Miranda de Goes Rodrigo Corrêa-Oliveira Érica Alessandra Rocha Alves Adriana Bozzi Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment. PLoS Neglected Tropical Diseases |
author_facet |
Vitor Hugo Simões Miranda Talita Rocha Gomes Dirli Emerick Eller Lorena de Cássia Neres Ferraz Ana Thereza Chaves Kelly Alves Bicalho Carlos Eduardo Calzavara Silva Alexander Birbrair Marcelo Antônio Pascoal Xavier Alfredo Miranda de Goes Rodrigo Corrêa-Oliveira Érica Alessandra Rocha Alves Adriana Bozzi |
author_sort |
Vitor Hugo Simões Miranda |
title |
Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment. |
title_short |
Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment. |
title_full |
Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment. |
title_fullStr |
Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment. |
title_full_unstemmed |
Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment. |
title_sort |
liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Neglected Tropical Diseases |
issn |
1935-2727 1935-2735 |
publishDate |
2020-08-01 |
description |
<h4>Background</h4>In view of the potential immunosuppressive and regenerative properties of mesenchymal stem cells (MSC), we investigated whether transplantation of adipose tissue-derived stem cells (ASC) could be used to control the granulomatous reaction in the liver of mice infected with Schistosoma mansoni after Praziquantel (PZQ) treatment.<h4>Methodology/prinicpal findings</h4>C57BL/6 mice infected with S. mansoni were treated with PZQ and transplanted intravenously with ASC from uninfected mice. Liver morpho-physiological and immunological analyses were performed. The combined PZQ/ASC therapy significantly reduced the volume of hepatic granulomas, as well as liver damage as measured by ALT levels. We also observed that ASC accelerated the progression of the granulomatous inflammation to the advanced/curative phase. The faster healing interfered with the expression of CD28 and CTLA-4 molecules in CD4+ T lymphocytes, and the levels of IL-10 and IL-17 cytokines, mainly in the livers of PZQ/ASC-treated mice.<h4>Conclusions</h4>Our results show that ASC therapy after PZQ treatment results in smaller granulomas with little tissue damage, suggesting the potential of ASC for the development of novel therapeutic approaches to minimize hepatic lesions as well as a granulomatous reaction following S. mansoni infection. Further studies using the chronic model of schistosomiasis are required to corroborate the therapeutic use of ASC for schistosomiasis. |
url |
https://doi.org/10.1371/journal.pntd.0008635 |
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