Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome
Reactive oxygen species (ROS) such as hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) can exert opposed effects depending on the dosage: low levels can be involved in signalling and adaptive processes, while higher levels can exert deleterious effects in cells and tissues. Our...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/10/12/1920 |
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doaj-795d14d622fc4dd5b55ad5f2fb2ac3bc |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Carla Busquets-Cortés Xavier Capó Emma Argelich Miguel D. Ferrer David Mateos Cristina Bouzas Manuela Abbate Josep A. Tur Antoni Sureda Antoni Pons |
| spellingShingle |
Carla Busquets-Cortés Xavier Capó Emma Argelich Miguel D. Ferrer David Mateos Cristina Bouzas Manuela Abbate Josep A. Tur Antoni Sureda Antoni Pons Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome Nutrients gene expression glucose oxidase hydrogen peroxide inflammation mitochondrial biogenesis neutrophils PBMCs ROS |
| author_facet |
Carla Busquets-Cortés Xavier Capó Emma Argelich Miguel D. Ferrer David Mateos Cristina Bouzas Manuela Abbate Josep A. Tur Antoni Sureda Antoni Pons |
| author_sort |
Carla Busquets-Cortés |
| title |
Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome |
| title_short |
Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome |
| title_full |
Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome |
| title_fullStr |
Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome |
| title_full_unstemmed |
Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic Syndrome |
| title_sort |
effects of millimolar steady-state hydrogen peroxide exposure on inflammatory and redox gene expression in immune cells from humans with metabolic syndrome |
| publisher |
MDPI AG |
| series |
Nutrients |
| issn |
2072-6643 |
| publishDate |
2018-12-01 |
| description |
Reactive oxygen species (ROS) such as hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) can exert opposed effects depending on the dosage: low levels can be involved in signalling and adaptive processes, while higher levels can exert deleterious effects in cells and tissues. Our aim was to emulate a chronic ex vivo oxidative stress situation through a 2 h exposure of immune cells to sustained H<sub>2</sub>O<sub>2</sub> produced by glucose oxidase (GOX), at high or low production rate, in order to determine dissimilar responses of peripheral blood mononuclear cells (PBMCs) and neutrophils on ROS and cytokine production, and mitochondrial dynamics-related proteins, pro/anti-inflammatory and anti-oxidant gene expression. Immune cells were obtained from subjects with metabolic syndrome. H<sub>2</sub>O<sub>2</sub> at low concentrations can trigger a transient anti-inflammatory adiponectin secretion and reduced gene expression of toll-like receptors (TLRs) in PBMCs but may act as a stimulator of proinflammatory genes (IL6, IL8) and mitochondrial dynamics-related proteins (Mtf2, NRF2, Tfam). H<sub>2</sub>O<sub>2</sub> at a high concentration enhances the expression of pro-inflammatory genes (TLR2 and IL1β) and diminishes the expression of mitochondrial dynamics-related proteins (Mtf1, Tfam) and antioxidant enzymes (Cu/Zn SOD) in PBMCs. The GOX treatments produce dissimilar changes in immune cells: Neutrophils were more resistant to H<sub>2</sub>O<sub>2</sub> effects and exhibited a more constant response in terms of gene expression than PBMCs. We observe emerging roles of H<sub>2</sub>O<sub>2</sub> in mitochondrial dynamics and redox and inflammation processes in immune cells. |
| topic |
gene expression glucose oxidase hydrogen peroxide inflammation mitochondrial biogenesis neutrophils PBMCs ROS |
| url |
https://www.mdpi.com/2072-6643/10/12/1920 |
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doaj-795d14d622fc4dd5b55ad5f2fb2ac3bc2020-11-24T22:59:42ZengMDPI AGNutrients2072-66432018-12-011012192010.3390/nu10121920nu10121920Effects of Millimolar Steady-State Hydrogen Peroxide Exposure on Inflammatory and Redox Gene Expression in Immune Cells from Humans with Metabolic SyndromeCarla Busquets-Cortés0Xavier Capó1Emma Argelich2Miguel D. Ferrer3David Mateos4Cristina Bouzas5Manuela Abbate6Josep A. Tur7Antoni Sureda8Antoni Pons9Research Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainResearch Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, 07122 Palma de Mallorca, SpainReactive oxygen species (ROS) such as hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) can exert opposed effects depending on the dosage: low levels can be involved in signalling and adaptive processes, while higher levels can exert deleterious effects in cells and tissues. Our aim was to emulate a chronic ex vivo oxidative stress situation through a 2 h exposure of immune cells to sustained H<sub>2</sub>O<sub>2</sub> produced by glucose oxidase (GOX), at high or low production rate, in order to determine dissimilar responses of peripheral blood mononuclear cells (PBMCs) and neutrophils on ROS and cytokine production, and mitochondrial dynamics-related proteins, pro/anti-inflammatory and anti-oxidant gene expression. Immune cells were obtained from subjects with metabolic syndrome. H<sub>2</sub>O<sub>2</sub> at low concentrations can trigger a transient anti-inflammatory adiponectin secretion and reduced gene expression of toll-like receptors (TLRs) in PBMCs but may act as a stimulator of proinflammatory genes (IL6, IL8) and mitochondrial dynamics-related proteins (Mtf2, NRF2, Tfam). H<sub>2</sub>O<sub>2</sub> at a high concentration enhances the expression of pro-inflammatory genes (TLR2 and IL1β) and diminishes the expression of mitochondrial dynamics-related proteins (Mtf1, Tfam) and antioxidant enzymes (Cu/Zn SOD) in PBMCs. The GOX treatments produce dissimilar changes in immune cells: Neutrophils were more resistant to H<sub>2</sub>O<sub>2</sub> effects and exhibited a more constant response in terms of gene expression than PBMCs. We observe emerging roles of H<sub>2</sub>O<sub>2</sub> in mitochondrial dynamics and redox and inflammation processes in immune cells.https://www.mdpi.com/2072-6643/10/12/1920gene expressionglucose oxidasehydrogen peroxideinflammationmitochondrial biogenesisneutrophilsPBMCsROS |