Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits
Abstract Descemet’s membrane (DM) helps maintain phenotype and function of corneal endothelial cells under physiological conditions, while little is known about the function of DM in corneal endothelial wound healing process. In the current study, we performed in vivo rabbit corneal endothelial cell...
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2017-08-01
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doaj-797519f7b29a44628ed3c0dc83b2fcb12020-12-08T02:00:27ZengNature Publishing GroupScientific Reports2045-23222017-08-017111310.1038/s41598-017-07557-2Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in RabbitsJingyao Chen0Zhiyuan Li1Liying Zhang2Shangkun Ou3Yanzi Wang4Xin He5Dulei Zou6Changkai Jia7Qianqian Hu8Shu Yang9Xian Li10Juan Li11Junqi Wang12Huimin Sun13Yongxiong Chen14Ying-Ting Zhu15Scheffer C. G. Tseng16Zuguo Liu17Wei Li18Eye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeOcular Surface CenterOcular Surface CenterEye Institute of Xiamen University, Xiamen University Medical CollegeEye Institute of Xiamen University, Xiamen University Medical CollegeAbstract Descemet’s membrane (DM) helps maintain phenotype and function of corneal endothelial cells under physiological conditions, while little is known about the function of DM in corneal endothelial wound healing process. In the current study, we performed in vivo rabbit corneal endothelial cell (CEC) injury via CEC scraping, in which DM remained intact after CECs removal, or via DM stripping, in which DM was removed together with CECs. We found rabbit corneas in the CEC scraping group healed with transparency restoration, while there was posterior fibrosis tissue formation in the corneas after DM stripping on day 14. Following CEC scraping on day 3, cells that had migrated toward the central cornea underwent a transient fibrotic endothelial-mesenchymal transition (EMT) which was reversed back to an endothelial phenotype on day 14. However, in the corneas injured via DM stripping, most of the cells in the posterior fibrosis tissue did not originate from the corneal endothelium, and they maintained fibroblastic phenotype on day 14. We concluded that corneal endothelial wound healing in rabbits has different outcomes depending upon the presence or absence of Descemet’s membrane. Descemet’s membrane supports corneal endothelial cell regeneration in rabbits after endothelial injury.https://doi.org/10.1038/s41598-017-07557-2 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jingyao Chen Zhiyuan Li Liying Zhang Shangkun Ou Yanzi Wang Xin He Dulei Zou Changkai Jia Qianqian Hu Shu Yang Xian Li Juan Li Junqi Wang Huimin Sun Yongxiong Chen Ying-Ting Zhu Scheffer C. G. Tseng Zuguo Liu Wei Li |
spellingShingle |
Jingyao Chen Zhiyuan Li Liying Zhang Shangkun Ou Yanzi Wang Xin He Dulei Zou Changkai Jia Qianqian Hu Shu Yang Xian Li Juan Li Junqi Wang Huimin Sun Yongxiong Chen Ying-Ting Zhu Scheffer C. G. Tseng Zuguo Liu Wei Li Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits Scientific Reports |
author_facet |
Jingyao Chen Zhiyuan Li Liying Zhang Shangkun Ou Yanzi Wang Xin He Dulei Zou Changkai Jia Qianqian Hu Shu Yang Xian Li Juan Li Junqi Wang Huimin Sun Yongxiong Chen Ying-Ting Zhu Scheffer C. G. Tseng Zuguo Liu Wei Li |
author_sort |
Jingyao Chen |
title |
Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits |
title_short |
Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits |
title_full |
Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits |
title_fullStr |
Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits |
title_full_unstemmed |
Descemet’s Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits |
title_sort |
descemet’s membrane supports corneal endothelial cell regeneration in rabbits |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Descemet’s membrane (DM) helps maintain phenotype and function of corneal endothelial cells under physiological conditions, while little is known about the function of DM in corneal endothelial wound healing process. In the current study, we performed in vivo rabbit corneal endothelial cell (CEC) injury via CEC scraping, in which DM remained intact after CECs removal, or via DM stripping, in which DM was removed together with CECs. We found rabbit corneas in the CEC scraping group healed with transparency restoration, while there was posterior fibrosis tissue formation in the corneas after DM stripping on day 14. Following CEC scraping on day 3, cells that had migrated toward the central cornea underwent a transient fibrotic endothelial-mesenchymal transition (EMT) which was reversed back to an endothelial phenotype on day 14. However, in the corneas injured via DM stripping, most of the cells in the posterior fibrosis tissue did not originate from the corneal endothelium, and they maintained fibroblastic phenotype on day 14. We concluded that corneal endothelial wound healing in rabbits has different outcomes depending upon the presence or absence of Descemet’s membrane. Descemet’s membrane supports corneal endothelial cell regeneration in rabbits after endothelial injury. |
url |
https://doi.org/10.1038/s41598-017-07557-2 |
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