Comparison of Anti-tumor Properties of the Cell walls of Saccharomyces Cerevisiae and Saccharomyces Boulardi Probiotics, individually and in Combination with Iron Nanoparticles on K562 Cancer cell line

• Main Authors: Salar Mokriani, Amir Tokmachi, Majid Nojavan
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2014-01-01
• Series: Basic & Clinical Cancer Research
• Subjects: Saccharomyces cerevisiae; S. boulardi; iron nanoparticles; Growth inhibition; K562 cell line
• Online Access: https://bccr.tums.ac.ir/index.php/bccrj/article/view/115

Background: Chronic myeloid leukemia is a common cancer in human and chemical therapy is a routine method for treatment that has some side effects. So the goal of this study was the use of natural compound such as cell wall obtained from Saccharomyces cerevisiae (S. cerevisiae) and Saccharomyces boulardi (S. boulardi) and iron nanoparticles on the growth inhibition of K562 cell line. Methods: For cell wall preparation both yeast were cultured in a basic medium at aerobic condition and 28 °C. Then the medium was centrifuged and precipitant washed with sterile buffer and the cells disrupted by sunicator. Also iron nanoparticles were prepared by biological method. Anti cancer property of different concentrations of the yeasts cell wall with iron nanoparticles were assayed by MTT and electrophoresis methods. Results: The results showed that S. boulardi cell wall significantly (p=0.029) inhibits the growth of K562 cell line compared to S. cerevisiae. Also iron nanoparticles significantly (p=0.021) inhibit K562 cell line. Results revealed that combination the iron nanoparticles with both yeasts cell wall decreased anti cancer property but this was not significant at the level of p<0.05. Conclusion: Based on this finding it should be concluded that combination of iron nanoparticles with Saccharomyces cell walls could inhibit the growth of K562 cell line in vitro. But these anti cancer properties would warrant further study on the clinical application of yeast cell wall.