Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen
Abstract Background Arsenic exposure has become a matter of worldwide concern, which is associated with immune-related diseases. However, little is known about its effect on inflammatory immune-related homeostasis. The purpose of our study was to understand the potential tuning of above responses ex...
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BMC
2020-07-01
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Series: | Cell & Bioscience |
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Online Access: | http://link.springer.com/article/10.1186/s13578-020-00448-6 |
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doaj-798f01618baf4c44b65906eedbc03a82 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nan Yan Guowei Xu Chenchen Zhang Xuping Liu Xin Li Lin Sun Da Wang Xiaoxu Duan Bing Li |
spellingShingle |
Nan Yan Guowei Xu Chenchen Zhang Xuping Liu Xin Li Lin Sun Da Wang Xiaoxu Duan Bing Li Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen Cell & Bioscience Arsenic Inflammation Immunosuppression Spleen |
author_facet |
Nan Yan Guowei Xu Chenchen Zhang Xuping Liu Xin Li Lin Sun Da Wang Xiaoxu Duan Bing Li |
author_sort |
Nan Yan |
title |
Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen |
title_short |
Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen |
title_full |
Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen |
title_fullStr |
Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen |
title_full_unstemmed |
Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen |
title_sort |
chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen |
publisher |
BMC |
series |
Cell & Bioscience |
issn |
2045-3701 |
publishDate |
2020-07-01 |
description |
Abstract Background Arsenic exposure has become a matter of worldwide concern, which is associated with immune-related diseases. However, little is known about its effect on inflammatory immune-related homeostasis. The purpose of our study was to understand the potential tuning of above responses exerted by chronic arsenic exposure. Methods Kunming mice were treated with 25 and 50 mg/L sodium arsenite for 1, 3 and 12 months via drinking water. At different endpoints of arsenic exposure, all animals and the whole spleen of the mice were weighed. The total arsenic levels of spleen were determined by the HPLC-HG-AFS method. Splenic NF-κB, MAPK and NRF2 protein levels by treatment of 25 mg/L NaAsO2 for 1, 3 and 12 months and 25 mg/L and 50 mg/L NaAsO2 for 12 months were assessed by western blot. Total RNA of spleen was isolated and relative mRNA levels of Foxp3, Il-10, Tnf-α, Il-6, Ifn-γ, Il-1β and Il-12 were measured by real-time PCR. Results Our results shown that NF-κB were continuously activated with treatment of 25 mg/L arsenic from 1, 3 to 12 months and 50 mg/L arsenic for 12 months. The transcription factor Foxp3 increased at 1 month but decreased at 3 and 12 months no matter 25 or 50 mg/L arsenic exposure. However, cytokine Il-10 always showed increased trend in mice treated with 25 or 50 mg/L arsenic for 1, 3 and 12 months. The transcriptional profiles of Tnf-α, Il-1β, Il-6, Ifn-γ and Il-12 revealed transient elevation at 1 and 3 months but shown significant decrease at 12 months on the whole. In addition, the sustained activation of inflammatory MAPK and anti-oxidative Nrf2 signaling pathways were observed in mice exposed to arsenic for 1, 3 and 12 months. Conclusion In summary, our experiment in vivo suggested chronic arsenic exposure induces the time-dependent modulation of the inflammation and immunosuppression in spleen, which may be related to the activation of Tregs induced by MAPK/NF-κB as well as the increased transcription level of Foxp3 and Il-10. |
topic |
Arsenic Inflammation Immunosuppression Spleen |
url |
http://link.springer.com/article/10.1186/s13578-020-00448-6 |
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doaj-798f01618baf4c44b65906eedbc03a822020-11-25T03:18:50ZengBMCCell & Bioscience2045-37012020-07-0110111010.1186/s13578-020-00448-6Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleenNan Yan0Guowei Xu1Chenchen Zhang2Xuping Liu3Xin Li4Lin Sun5Da Wang6Xiaoxu Duan7Bing Li8Environment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-Related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical UniversityEnvironment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-Related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical UniversityEnvironment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-Related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical UniversityEnvironment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-Related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical UniversityEnvironment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-Related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical UniversityEnvironment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-Related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical UniversityEnvironment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-Related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical UniversityDepartment of Toxicology, School of Public Health, Shenyang Medical CollegeEnvironment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-Related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical UniversityAbstract Background Arsenic exposure has become a matter of worldwide concern, which is associated with immune-related diseases. However, little is known about its effect on inflammatory immune-related homeostasis. The purpose of our study was to understand the potential tuning of above responses exerted by chronic arsenic exposure. Methods Kunming mice were treated with 25 and 50 mg/L sodium arsenite for 1, 3 and 12 months via drinking water. At different endpoints of arsenic exposure, all animals and the whole spleen of the mice were weighed. The total arsenic levels of spleen were determined by the HPLC-HG-AFS method. Splenic NF-κB, MAPK and NRF2 protein levels by treatment of 25 mg/L NaAsO2 for 1, 3 and 12 months and 25 mg/L and 50 mg/L NaAsO2 for 12 months were assessed by western blot. Total RNA of spleen was isolated and relative mRNA levels of Foxp3, Il-10, Tnf-α, Il-6, Ifn-γ, Il-1β and Il-12 were measured by real-time PCR. Results Our results shown that NF-κB were continuously activated with treatment of 25 mg/L arsenic from 1, 3 to 12 months and 50 mg/L arsenic for 12 months. The transcription factor Foxp3 increased at 1 month but decreased at 3 and 12 months no matter 25 or 50 mg/L arsenic exposure. However, cytokine Il-10 always showed increased trend in mice treated with 25 or 50 mg/L arsenic for 1, 3 and 12 months. The transcriptional profiles of Tnf-α, Il-1β, Il-6, Ifn-γ and Il-12 revealed transient elevation at 1 and 3 months but shown significant decrease at 12 months on the whole. In addition, the sustained activation of inflammatory MAPK and anti-oxidative Nrf2 signaling pathways were observed in mice exposed to arsenic for 1, 3 and 12 months. Conclusion In summary, our experiment in vivo suggested chronic arsenic exposure induces the time-dependent modulation of the inflammation and immunosuppression in spleen, which may be related to the activation of Tregs induced by MAPK/NF-κB as well as the increased transcription level of Foxp3 and Il-10.http://link.springer.com/article/10.1186/s13578-020-00448-6ArsenicInflammationImmunosuppressionSpleen |