On‐site genetic analysis for species identification using lab‐on‐a‐chip
Abstract This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (Ceratotherium simum). The development of a microfluidic device, which can be used in the field, offers a portable and cost‐effective solution for DNA analysis and s...
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Online Access: | https://doi.org/10.1002/ece3.7053 |
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doaj-7995c337de594df089788d05181135072021-04-02T19:33:29ZengWileyEcology and Evolution2045-77582021-02-011141535154310.1002/ece3.7053On‐site genetic analysis for species identification using lab‐on‐a‐chipRyan Wimbles0Louise M. Melling1Bradley Cain2Naomi Davies3Jason Doherty4Bridget Johnson5Kirsty J. Shaw6Department of Natural Sciences Manchester Metropolitan University Manchester UKDepartment of Natural Sciences Manchester Metropolitan University Manchester UKDepartment of Natural Sciences Manchester Metropolitan University Manchester UKKnowsley Safari Prescot UKKnowsley Safari Prescot UKKnowsley Safari Prescot UKDepartment of Natural Sciences Manchester Metropolitan University Manchester UKAbstract This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (Ceratotherium simum). The development of a microfluidic device, which can be used in the field, offers a portable and cost‐effective solution for DNA analysis and species identification to aid conservation efforts. Optimization of the DNA extraction processes produced equivalent yields compared to conventional kit‐based methods within just 5 minutes. The use of a color‐changing loop‐mediated isothermal amplification reaction for simultaneous detection of the cytochrome B sequence of C. simum enabled positive results to be obtained within as little as 30 minutes. Field testing was performed at Knowsley Safari to demonstrate real‐world applicability of the microfluidic device for testing of biological samples.https://doi.org/10.1002/ece3.7053conservation geneticsidentificationlab‐on‐a‐chipmicrofluidicsrhinoceros |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ryan Wimbles Louise M. Melling Bradley Cain Naomi Davies Jason Doherty Bridget Johnson Kirsty J. Shaw |
spellingShingle |
Ryan Wimbles Louise M. Melling Bradley Cain Naomi Davies Jason Doherty Bridget Johnson Kirsty J. Shaw On‐site genetic analysis for species identification using lab‐on‐a‐chip Ecology and Evolution conservation genetics identification lab‐on‐a‐chip microfluidics rhinoceros |
author_facet |
Ryan Wimbles Louise M. Melling Bradley Cain Naomi Davies Jason Doherty Bridget Johnson Kirsty J. Shaw |
author_sort |
Ryan Wimbles |
title |
On‐site genetic analysis for species identification using lab‐on‐a‐chip |
title_short |
On‐site genetic analysis for species identification using lab‐on‐a‐chip |
title_full |
On‐site genetic analysis for species identification using lab‐on‐a‐chip |
title_fullStr |
On‐site genetic analysis for species identification using lab‐on‐a‐chip |
title_full_unstemmed |
On‐site genetic analysis for species identification using lab‐on‐a‐chip |
title_sort |
on‐site genetic analysis for species identification using lab‐on‐a‐chip |
publisher |
Wiley |
series |
Ecology and Evolution |
issn |
2045-7758 |
publishDate |
2021-02-01 |
description |
Abstract This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (Ceratotherium simum). The development of a microfluidic device, which can be used in the field, offers a portable and cost‐effective solution for DNA analysis and species identification to aid conservation efforts. Optimization of the DNA extraction processes produced equivalent yields compared to conventional kit‐based methods within just 5 minutes. The use of a color‐changing loop‐mediated isothermal amplification reaction for simultaneous detection of the cytochrome B sequence of C. simum enabled positive results to be obtained within as little as 30 minutes. Field testing was performed at Knowsley Safari to demonstrate real‐world applicability of the microfluidic device for testing of biological samples. |
topic |
conservation genetics identification lab‐on‐a‐chip microfluidics rhinoceros |
url |
https://doi.org/10.1002/ece3.7053 |
work_keys_str_mv |
AT ryanwimbles onsitegeneticanalysisforspeciesidentificationusinglabonachip AT louisemmelling onsitegeneticanalysisforspeciesidentificationusinglabonachip AT bradleycain onsitegeneticanalysisforspeciesidentificationusinglabonachip AT naomidavies onsitegeneticanalysisforspeciesidentificationusinglabonachip AT jasondoherty onsitegeneticanalysisforspeciesidentificationusinglabonachip AT bridgetjohnson onsitegeneticanalysisforspeciesidentificationusinglabonachip AT kirstyjshaw onsitegeneticanalysisforspeciesidentificationusinglabonachip |
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1721548603318599680 |