Isolation and Characterization of Neural Progenitor Cells From Bone Marrow in Cell Replacement Therapy of Brain Injury
Many studies supported that bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into neural cells, but few researchers detected mature and function of nerve cells, especially in vivo study. Some researchers even suggested that BM-MSCs transplantation would not be able to differentiate int...
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doaj-7995de5b85d44bb7bc6571af4ebfe4ba2020-11-25T01:40:39ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022020-03-011410.3389/fncel.2020.00049517922Isolation and Characterization of Neural Progenitor Cells From Bone Marrow in Cell Replacement Therapy of Brain InjuryWen-fang Bai0Yuling Zhang1Yuling Zhang2Weicheng Xu3Weikun Li4Meihui Li5Fengying Yuan6Xun Luo7Xun Luo8Mingsheng Zhang9Department of Rehabilitation Medicine, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Institute of Geriatrics, Guangzhou, ChinaSchool of Medical Instrument and Food Engineering, The University of Shanghai for Science and Technology, Shanghai, ChinaStroke Biological Recovery Laboratory, Spaulding Rehabilitation Hospital, a Teaching Affiliate of Harvard Medical School, Charlestown, MA, United StatesDepartment of Rehabilitation Medicine, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Institute of Geriatrics, Guangzhou, ChinaDepartment of Rehabilitation Medicine, Zengcheng District People’s Hospital of Guangzhou, Guangzhou, ChinaDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, ChinaDepartment of Rehabilitation Medicine, Guangdong Provincial Hospital of Integrated Traditional Chinese and Western Medicine, Foshan, ChinaKerry Rehabilitation Medicine Research Institute, Shenzhen, ChinaShenzhen Sanming Project Group, Spaulding Rehabilitation Hospital, a Teaching Affiliate of Harvard Medical School, Charlestown, MA, United StatesDepartment of Rehabilitation Medicine, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Institute of Geriatrics, Guangzhou, ChinaMany studies supported that bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into neural cells, but few researchers detected mature and function of nerve cells, especially in vivo study. Some researchers even suggested that BM-MSCs transplantation would not be able to differentiate into functional neural cells. To figure out the dispute, this study examined bone marrow-derived sphere-like cells, harvested via neural stem cell suspension culture, then identified as bone marrow-derived neural progenitor cells (BM-NPCs) by finding the expression of neural progenitor cells genes and proteins, neural progenitor cells characteristic and nerve cell differentiation induced through both methods. Moreover, BM-NPCs transplantation showed long-term survival and improved the ethological and histological indexes of brain injury rats, demonstrating functional nervous cells differentiated from BM-NPCs. These in vitro and in vivo results confirmed BM-NPCs differentiating into mature and functional nerve cells. This study provided valuable experimental data for BM-NPCs, suggesting a potential alternative treatment of central nervous injury disease.https://www.frontiersin.org/article/10.3389/fncel.2020.00049/fullmesenchymal stem cellsbone marrow-derived neural progenitor cellsnerve cellsdifferentiationcell transplantation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen-fang Bai Yuling Zhang Yuling Zhang Weicheng Xu Weikun Li Meihui Li Fengying Yuan Xun Luo Xun Luo Mingsheng Zhang |
spellingShingle |
Wen-fang Bai Yuling Zhang Yuling Zhang Weicheng Xu Weikun Li Meihui Li Fengying Yuan Xun Luo Xun Luo Mingsheng Zhang Isolation and Characterization of Neural Progenitor Cells From Bone Marrow in Cell Replacement Therapy of Brain Injury Frontiers in Cellular Neuroscience mesenchymal stem cells bone marrow-derived neural progenitor cells nerve cells differentiation cell transplantation |
author_facet |
Wen-fang Bai Yuling Zhang Yuling Zhang Weicheng Xu Weikun Li Meihui Li Fengying Yuan Xun Luo Xun Luo Mingsheng Zhang |
author_sort |
Wen-fang Bai |
title |
Isolation and Characterization of Neural Progenitor Cells From Bone Marrow in Cell Replacement Therapy of Brain Injury |
title_short |
Isolation and Characterization of Neural Progenitor Cells From Bone Marrow in Cell Replacement Therapy of Brain Injury |
title_full |
Isolation and Characterization of Neural Progenitor Cells From Bone Marrow in Cell Replacement Therapy of Brain Injury |
title_fullStr |
Isolation and Characterization of Neural Progenitor Cells From Bone Marrow in Cell Replacement Therapy of Brain Injury |
title_full_unstemmed |
Isolation and Characterization of Neural Progenitor Cells From Bone Marrow in Cell Replacement Therapy of Brain Injury |
title_sort |
isolation and characterization of neural progenitor cells from bone marrow in cell replacement therapy of brain injury |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2020-03-01 |
description |
Many studies supported that bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into neural cells, but few researchers detected mature and function of nerve cells, especially in vivo study. Some researchers even suggested that BM-MSCs transplantation would not be able to differentiate into functional neural cells. To figure out the dispute, this study examined bone marrow-derived sphere-like cells, harvested via neural stem cell suspension culture, then identified as bone marrow-derived neural progenitor cells (BM-NPCs) by finding the expression of neural progenitor cells genes and proteins, neural progenitor cells characteristic and nerve cell differentiation induced through both methods. Moreover, BM-NPCs transplantation showed long-term survival and improved the ethological and histological indexes of brain injury rats, demonstrating functional nervous cells differentiated from BM-NPCs. These in vitro and in vivo results confirmed BM-NPCs differentiating into mature and functional nerve cells. This study provided valuable experimental data for BM-NPCs, suggesting a potential alternative treatment of central nervous injury disease. |
topic |
mesenchymal stem cells bone marrow-derived neural progenitor cells nerve cells differentiation cell transplantation |
url |
https://www.frontiersin.org/article/10.3389/fncel.2020.00049/full |
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