New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers
Abstract European badgers (Meles meles) have been identified as wildlife reservoirs for Mycobacterium bovis in the UK and Ireland, and may also have a role in the epidemiology of animal tuberculosis in other European regions. Thus, detection of M. bovis‐infected badgers may be required for the purpo...
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doaj-799879fbe39d492b90d62590035605d12020-11-24T21:52:15ZengWileyVeterinary Medicine and Science2053-10952019-02-0151616910.1002/vms3.134New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgersJose A. Infantes‐Lorenzo0Dipesh Dave1Immaculada Moreno2Paul Anderson3Sandrine Lesellier4Eamonn Gormley5Lucas Dominguez6Ana Balseiro7Christian Gortázar8Mercedes Dominguez9Francisco J. Salguero10VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid SpainBacteriology Department Animal and Plant Health Agency Addlestone Surrey UKUnidad de Inmunología Microbiana Centro Nacional de Microbiología Instituto de Salud Carlos III Majadahonda Madrid SpainBacteriology Department Animal and Plant Health Agency Addlestone Surrey UKBacteriology Department Animal and Plant Health Agency Addlestone Surrey UKSchool of Veterinary Medicine University College Dublin (UCD) Dublin IrelandVISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid SpainCentro de Biotecnología Animal Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA) Deva‐Gijón Asturias SpainSaBio Instituto de Investigación en Recursos Cinegéticos IREC (CSIC‐UCLM‐JCCM) Ciudad Real SpainUnidad de Inmunología Microbiana Centro Nacional de Microbiología Instituto de Salud Carlos III Majadahonda Madrid SpainDepartment of Pathology and Infectious Diseases School of Veterinary Medicine University of Surrey Guildford UKAbstract European badgers (Meles meles) have been identified as wildlife reservoirs for Mycobacterium bovis in the UK and Ireland, and may also have a role in the epidemiology of animal tuberculosis in other European regions. Thus, detection of M. bovis‐infected badgers may be required for the purposes of surveillance and monitoring of disease levels in infected populations. Current serological assays to detect M. bovis infection in live badgers, while rapid and inexpensive, show limited diagnostic sensitivity. Here we describe and evaluate new ELISA platforms for the recognition of the P22 multiprotein complex derived from the purified protein derivative (PPD) of M. bovis. The recognition of IgG against P22 multiprotein complex derived from PPD‐B was tested by ELISA in the serum of badgers from the UK, Ireland and Spain. TB infection in the badgers was indicated by the presence of M. bovis in tissues by culture and histology at post‐mortem examination and TB‐free status was established by repeated negativity in the interferon γ release assay (IGRA). In experimentally infected badgers, humoral antibody responses against P22 developed within 45 days post‐infection. The ELISA tests showed estimated sensitivity levels of 74–82% in experimentally and naturally infected badgers with specificities ranging from 75% to 100% depending on the badger population tested. The P22 multi‐antigen based ELISAs provide a sensitive and specific test platform for improved tuberculosis surveillance in badgers.https://doi.org/10.1002/vms3.134BadgersdiagnosisP22 ELISAtuberculosiswildlife reservoir |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jose A. Infantes‐Lorenzo Dipesh Dave Immaculada Moreno Paul Anderson Sandrine Lesellier Eamonn Gormley Lucas Dominguez Ana Balseiro Christian Gortázar Mercedes Dominguez Francisco J. Salguero |
spellingShingle |
Jose A. Infantes‐Lorenzo Dipesh Dave Immaculada Moreno Paul Anderson Sandrine Lesellier Eamonn Gormley Lucas Dominguez Ana Balseiro Christian Gortázar Mercedes Dominguez Francisco J. Salguero New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers Veterinary Medicine and Science Badgers diagnosis P22 ELISA tuberculosis wildlife reservoir |
author_facet |
Jose A. Infantes‐Lorenzo Dipesh Dave Immaculada Moreno Paul Anderson Sandrine Lesellier Eamonn Gormley Lucas Dominguez Ana Balseiro Christian Gortázar Mercedes Dominguez Francisco J. Salguero |
author_sort |
Jose A. Infantes‐Lorenzo |
title |
New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers |
title_short |
New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers |
title_full |
New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers |
title_fullStr |
New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers |
title_full_unstemmed |
New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers |
title_sort |
new serological platform for detecting antibodies against mycobacterium tuberculosis complex in european badgers |
publisher |
Wiley |
series |
Veterinary Medicine and Science |
issn |
2053-1095 |
publishDate |
2019-02-01 |
description |
Abstract European badgers (Meles meles) have been identified as wildlife reservoirs for Mycobacterium bovis in the UK and Ireland, and may also have a role in the epidemiology of animal tuberculosis in other European regions. Thus, detection of M. bovis‐infected badgers may be required for the purposes of surveillance and monitoring of disease levels in infected populations. Current serological assays to detect M. bovis infection in live badgers, while rapid and inexpensive, show limited diagnostic sensitivity. Here we describe and evaluate new ELISA platforms for the recognition of the P22 multiprotein complex derived from the purified protein derivative (PPD) of M. bovis. The recognition of IgG against P22 multiprotein complex derived from PPD‐B was tested by ELISA in the serum of badgers from the UK, Ireland and Spain. TB infection in the badgers was indicated by the presence of M. bovis in tissues by culture and histology at post‐mortem examination and TB‐free status was established by repeated negativity in the interferon γ release assay (IGRA). In experimentally infected badgers, humoral antibody responses against P22 developed within 45 days post‐infection. The ELISA tests showed estimated sensitivity levels of 74–82% in experimentally and naturally infected badgers with specificities ranging from 75% to 100% depending on the badger population tested. The P22 multi‐antigen based ELISAs provide a sensitive and specific test platform for improved tuberculosis surveillance in badgers. |
topic |
Badgers diagnosis P22 ELISA tuberculosis wildlife reservoir |
url |
https://doi.org/10.1002/vms3.134 |
work_keys_str_mv |
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