Genome Sequencing of <em>Streptomyces olivaceus</em> SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining
Marine-sourced actinomycete genus <i>Streptomyces</i> continues to be an important source of new natural products. Here we report the complete genome sequence of deep-sea-derived <i>Streptomyces olivaceus</i> SCSIO T05, harboring 37 putative biosynthetic gene clusters (BGCs)....
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doaj-799ed0625397450491a86b80ef6aa7ca2020-11-25T03:25:11ZengMDPI AGMarine Drugs1660-33972019-10-01171059310.3390/md17100593md17100593Genome Sequencing of <em>Streptomyces olivaceus</em> SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome MiningChunyan Zhang0Wenjuan Ding1Xiangjing Qin2Jianhua Ju3CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaMarine-sourced actinomycete genus <i>Streptomyces</i> continues to be an important source of new natural products. Here we report the complete genome sequence of deep-sea-derived <i>Streptomyces olivaceus</i> SCSIO T05, harboring 37 putative biosynthetic gene clusters (BGCs). A cryptic BGC for type I polyketides was activated by metabolic engineering methods, enabling the discovery of a known compound, lobophorin CR4 (<b>1</b>). Genome mining yielded a putative lobophorin BGC (<i>lbp</i>) that missed the functional FAD-dependent oxidoreductase to generate the <span style="font-variant: small-caps;">d</span>-kijanose, leading to the production of lobophorin CR4 without the attachment of <span style="font-variant: small-caps;">d</span>-kijanose to C17-OH. Using the gene-disruption method, we confirmed that the <i>lbp</i> BGC accounts for lobophorin biosynthesis. We conclude that metabolic engineering and genome mining provide an effective approach to activate cryptic BGCs.https://www.mdpi.com/1660-3397/17/10/593genome sequencinggene disruptionlobophorinmetabolic engineeringgenome mining |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chunyan Zhang Wenjuan Ding Xiangjing Qin Jianhua Ju |
spellingShingle |
Chunyan Zhang Wenjuan Ding Xiangjing Qin Jianhua Ju Genome Sequencing of <em>Streptomyces olivaceus</em> SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining Marine Drugs genome sequencing gene disruption lobophorin metabolic engineering genome mining |
author_facet |
Chunyan Zhang Wenjuan Ding Xiangjing Qin Jianhua Ju |
author_sort |
Chunyan Zhang |
title |
Genome Sequencing of <em>Streptomyces olivaceus</em> SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining |
title_short |
Genome Sequencing of <em>Streptomyces olivaceus</em> SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining |
title_full |
Genome Sequencing of <em>Streptomyces olivaceus</em> SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining |
title_fullStr |
Genome Sequencing of <em>Streptomyces olivaceus</em> SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining |
title_full_unstemmed |
Genome Sequencing of <em>Streptomyces olivaceus</em> SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining |
title_sort |
genome sequencing of <em>streptomyces olivaceus</em> scsio t05 and activated production of lobophorin cr4 via metabolic engineering and genome mining |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2019-10-01 |
description |
Marine-sourced actinomycete genus <i>Streptomyces</i> continues to be an important source of new natural products. Here we report the complete genome sequence of deep-sea-derived <i>Streptomyces olivaceus</i> SCSIO T05, harboring 37 putative biosynthetic gene clusters (BGCs). A cryptic BGC for type I polyketides was activated by metabolic engineering methods, enabling the discovery of a known compound, lobophorin CR4 (<b>1</b>). Genome mining yielded a putative lobophorin BGC (<i>lbp</i>) that missed the functional FAD-dependent oxidoreductase to generate the <span style="font-variant: small-caps;">d</span>-kijanose, leading to the production of lobophorin CR4 without the attachment of <span style="font-variant: small-caps;">d</span>-kijanose to C17-OH. Using the gene-disruption method, we confirmed that the <i>lbp</i> BGC accounts for lobophorin biosynthesis. We conclude that metabolic engineering and genome mining provide an effective approach to activate cryptic BGCs. |
topic |
genome sequencing gene disruption lobophorin metabolic engineering genome mining |
url |
https://www.mdpi.com/1660-3397/17/10/593 |
work_keys_str_mv |
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