Process Intensification for an Insect Antimicrobial Peptide Elastin-Like Polypeptide Fusion Produced in Redox-Engineered Escherichia coli
Peptides and proteins containing disulfide bonds can be produced in Escherichia coli by targeting the oxidizing periplasm, co-expressing isomerases or chaperons, refolding from inclusion bodies, or by using redox-engineered E. coli strains. Thus far, protein expression in glutathione reductase and t...
Main Authors: | Mathias Joachim, Nicolas Maguire, Johannes Schäfer, Doreen Gerlach, Peter Czermak |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-06-01
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Series: | Frontiers in Bioengineering and Biotechnology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00150/full |
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