KINETIC STUDY OF THE ENZYME CONVERSION OF STEAM EXPLODED PAULOWNIA TOMENTOSA TO GLUCOSE
Paulownia Tomentosa was pretreated by steam explosion. The cellulase complex NS 50013 and the β-glucosidase NS 50010 of Novozymes AS were used for the enzymatic conversion of cellulose to glucose. The kinetics of enzyme conversion was studied using the exponential kinetic equation valid for processe...
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North Carolina State University
2011-11-01
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doaj-79f599c0366e4b6385fd0a8bdb9257472020-11-24T23:00:01ZengNorth Carolina State UniversityBioResources1930-21262011-11-0171412421KINETIC STUDY OF THE ENZYME CONVERSION OF STEAM EXPLODED PAULOWNIA TOMENTOSA TO GLUCOSEGreta Radeva,Ivo Valchev,Stoiko Petrin,Eva Valcheva,Petya TsekovaPaulownia Tomentosa was pretreated by steam explosion. The cellulase complex NS 50013 and the β-glucosidase NS 50010 of Novozymes AS were used for the enzymatic conversion of cellulose to glucose. The kinetics of enzyme conversion was studied using the exponential kinetic equation valid for processes taking place at uniformly inhomogeneous surfaces. The kinetic coefficient of inhomogeneity accounts for the energy and entropy inhomogeneity of the system and depends on the temperature. It was established that both the activation energy and the pre-exponential factor increase simultaneously with increasing of conversion degree. A compensation effect between pre-exponential factor and activation energy was observed. The energetic hindrances established cannot be completely compensated by the positive effect of the pre-exponential factor increase. Hence, the activation energy has a determining influence on how quickly the rate of hydrolysis decreases.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_1_0412_Radeva_VPVT_Kinetic_Enzym_Conver_Steam_Expl_Paulownia/1314PaulowniaCellulaseKineticsExponential equationCompensation effect |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Greta Radeva, Ivo Valchev, Stoiko Petrin, Eva Valcheva, Petya Tsekova |
spellingShingle |
Greta Radeva, Ivo Valchev, Stoiko Petrin, Eva Valcheva, Petya Tsekova KINETIC STUDY OF THE ENZYME CONVERSION OF STEAM EXPLODED PAULOWNIA TOMENTOSA TO GLUCOSE BioResources Paulownia Cellulase Kinetics Exponential equation Compensation effect |
author_facet |
Greta Radeva, Ivo Valchev, Stoiko Petrin, Eva Valcheva, Petya Tsekova |
author_sort |
Greta Radeva, |
title |
KINETIC STUDY OF THE ENZYME CONVERSION OF STEAM EXPLODED PAULOWNIA TOMENTOSA TO GLUCOSE |
title_short |
KINETIC STUDY OF THE ENZYME CONVERSION OF STEAM EXPLODED PAULOWNIA TOMENTOSA TO GLUCOSE |
title_full |
KINETIC STUDY OF THE ENZYME CONVERSION OF STEAM EXPLODED PAULOWNIA TOMENTOSA TO GLUCOSE |
title_fullStr |
KINETIC STUDY OF THE ENZYME CONVERSION OF STEAM EXPLODED PAULOWNIA TOMENTOSA TO GLUCOSE |
title_full_unstemmed |
KINETIC STUDY OF THE ENZYME CONVERSION OF STEAM EXPLODED PAULOWNIA TOMENTOSA TO GLUCOSE |
title_sort |
kinetic study of the enzyme conversion of steam exploded paulownia tomentosa to glucose |
publisher |
North Carolina State University |
series |
BioResources |
issn |
1930-2126 |
publishDate |
2011-11-01 |
description |
Paulownia Tomentosa was pretreated by steam explosion. The cellulase complex NS 50013 and the β-glucosidase NS 50010 of Novozymes AS were used for the enzymatic conversion of cellulose to glucose. The kinetics of enzyme conversion was studied using the exponential kinetic equation valid for processes taking place at uniformly inhomogeneous surfaces. The kinetic coefficient of inhomogeneity accounts for the energy and entropy inhomogeneity of the system and depends on the temperature. It was established that both the activation energy and the pre-exponential factor increase simultaneously with increasing of conversion degree. A compensation effect between pre-exponential factor and activation energy was observed. The energetic hindrances established cannot be completely compensated by the positive effect of the pre-exponential factor increase. Hence, the activation energy has a determining influence on how quickly the rate of hydrolysis decreases. |
topic |
Paulownia Cellulase Kinetics Exponential equation Compensation effect |
url |
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_1_0412_Radeva_VPVT_Kinetic_Enzym_Conver_Steam_Expl_Paulownia/1314 |
work_keys_str_mv |
AT gretaradeva kineticstudyoftheenzymeconversionofsteamexplodedpaulowniatomentosatoglucose AT ivovalchev kineticstudyoftheenzymeconversionofsteamexplodedpaulowniatomentosatoglucose AT stoikopetrin kineticstudyoftheenzymeconversionofsteamexplodedpaulowniatomentosatoglucose AT evavalcheva kineticstudyoftheenzymeconversionofsteamexplodedpaulowniatomentosatoglucose AT petyatsekova kineticstudyoftheenzymeconversionofsteamexplodedpaulowniatomentosatoglucose |
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1725642883269132288 |