An analysis of expression patterns of genes encoding proteins with catalytic activities

• Main Authors: Ekinci Deniz, Emircupani Tufan, Comakli Veysel, Gulcin Ilhami, Budak Harun, Ozdemir Hasan, Beydemir Sukru, Ciftci Mehmet, Hernandez Ana, Cankaya Murat, Kuzu Muslum, Jiang Qiuhong, Eichele Gregor, Kufrevioglu Omer
• Format: Article
• Language: English
• Published: BMC 2007-07-01
• Series: BMC Genomics
• Online Access: http://www.biomedcentral.com/1471-2164/8/232

<p>Abstract</p> <p>Background</p> <p><it>In situ </it>hybridization (ISH) is a powerful method for visualizing gene expression patterns at the organismal level with cellular resolution. When automated, it is capable of determining the expression of a large number of genes.</p> <p>Results</p> <p>The expression patterns of 662 genes that encode enzymes were determined by ISH in the mid-gestation mouse embryo, a stage that models the complexity of the adult organism. Forty-five percent of transcripts encoding metabolic enzymes (n = 297) showed a regional expression pattern. A similar percentage was found for the 190 kinases that were also analyzed. Many mRNAs encoding glycolytic and TCA cycle enzymes exhibited a characteristic expression pattern. The annotated expression patterns were deposited on the Genepaint database and are retrievable by user-defined queries including gene name and sites of expression.</p> <p>Conclusion</p> <p>The 662 expression patterns discussed here comprised gene products with activities associated with catalysis. Preliminary analysis of these data revealed that a significant number of genes encoding housekeeping functions such as biosynthesis and catabolism were expressed regionally, so they could be used as tissue-specific gene markers. We found no difference in tissue specificity between mRNAs encoding housekeeping functions and those encoding components of signal transduction pathways, as exemplified by the kinases.</p>