An In-Vitro Study of the Antibacterial Efficacy of Cavity Liners Against Streptococcus Mutans and Lactobacillus Casei
Background and aim: The main factor that influences the durability of dental restorations is secondary caries. Antibacterial activity of dental materials is important from the clinical aspect, as it might inhibit recurrent caries. The aim of the present study was to compare the antibacterial activit...
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doaj-7a047ba8983d4383bb9040d74542e5d32020-11-24T22:28:49ZengIslamic Azad UniversityJournal of Research in Dental and Maxillofacial Sciences2383-27542017-07-01222328An In-Vitro Study of the Antibacterial Efficacy of Cavity Liners Against Streptococcus Mutans and Lactobacillus CaseiN kazeroonizadeh0M Kazemian1P Mirzakoochaki brojen2 Postgraduate student, Department of operative dentistry, faculty of dentistry, Isfahan (Khorasgan ) branch, Islamic Azad university, Isfahan , Iran Azad university of esfahan Operative dentistry Dept, faculty of dentistry, Isfahan (Khorasgan ) branch, Islamic Azad university, Isfahan , Iran Background and aim: The main factor that influences the durability of dental restorations is secondary caries. Antibacterial activity of dental materials is important from the clinical aspect, as it might inhibit recurrent caries. The aim of the present study was to compare the antibacterial activity of four fluoride-releasing dental cavity liners against Streptococcus mutans (S. mutans) and Lactobacillus casei (L. casei). Materials and methods: In this experimental in-vitro study, the agar diffusion test was used to compare the antibacterial efficacy of four dental cavity liners against S. mutans and L. casei. Indicator strains of S. mutans (ATCC35668) and L. casei (ATCC393) were obtained in the form of lyophilized culture. They were grown separately in 15 ml of Brain Heart Infusion (BHI) agar at 37 °C for 48 hours. Antibacterial activities of Ionobond (VOCO), Ionoseal (VOCO), Ionosit (DMG), and Vitrebond (3M) dental cavity liners were evaluated at 24 and 48 hours and at 7 days by measuring the diameter of the inhibition zone in millimeters (mm). Data were collected and analyzed using the repeated measure ANOVA and T-test. The level of significance was set at p<0.05. Results: The antibacterial efficacy of the four studied dental cavity liners differed at different time intervals (p<0.001), but there were no statically significant differences in the antibacterial activity against the two bacteria types (p=0.342), or between the four types of dental cavity liners (p=0.07). Conclusion: According to the results of the present research, the antibacterial activities of Ionobond, Ionoseal, Ionosit and Vitrebond dental cavity liners were not significantly different and decreased over time.http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-567-1&slc_lang=en&sid=1Dental linersAntibacterial effectStreptococcus mutansLactobacillus caseiFluoride-releasing dental materials |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
N kazeroonizadeh M Kazemian P Mirzakoochaki brojen |
spellingShingle |
N kazeroonizadeh M Kazemian P Mirzakoochaki brojen An In-Vitro Study of the Antibacterial Efficacy of Cavity Liners Against Streptococcus Mutans and Lactobacillus Casei Journal of Research in Dental and Maxillofacial Sciences Dental liners Antibacterial effect Streptococcus mutans Lactobacillus casei Fluoride-releasing dental materials |
author_facet |
N kazeroonizadeh M Kazemian P Mirzakoochaki brojen |
author_sort |
N kazeroonizadeh |
title |
An In-Vitro Study of the Antibacterial Efficacy of Cavity Liners Against Streptococcus Mutans and Lactobacillus Casei |
title_short |
An In-Vitro Study of the Antibacterial Efficacy of Cavity Liners Against Streptococcus Mutans and Lactobacillus Casei |
title_full |
An In-Vitro Study of the Antibacterial Efficacy of Cavity Liners Against Streptococcus Mutans and Lactobacillus Casei |
title_fullStr |
An In-Vitro Study of the Antibacterial Efficacy of Cavity Liners Against Streptococcus Mutans and Lactobacillus Casei |
title_full_unstemmed |
An In-Vitro Study of the Antibacterial Efficacy of Cavity Liners Against Streptococcus Mutans and Lactobacillus Casei |
title_sort |
in-vitro study of the antibacterial efficacy of cavity liners against streptococcus mutans and lactobacillus casei |
publisher |
Islamic Azad University |
series |
Journal of Research in Dental and Maxillofacial Sciences |
issn |
2383-2754 |
publishDate |
2017-07-01 |
description |
Background and aim: The main factor that influences the durability of dental restorations is secondary caries. Antibacterial activity of dental materials is important from the clinical aspect, as it might inhibit recurrent caries. The aim of the present study was to compare the antibacterial activity of four fluoride-releasing dental cavity liners against Streptococcus mutans (S. mutans) and Lactobacillus casei (L. casei).
Materials and methods: In this experimental in-vitro study, the agar diffusion test was used to compare the antibacterial efficacy of four dental cavity liners against S. mutans and L. casei. Indicator strains of S. mutans (ATCC35668) and L. casei (ATCC393) were obtained in the form of lyophilized culture. They were grown separately in 15 ml of Brain Heart Infusion (BHI) agar at 37 °C for 48 hours. Antibacterial activities of Ionobond (VOCO), Ionoseal (VOCO), Ionosit (DMG), and Vitrebond (3M) dental cavity liners were evaluated at 24 and 48 hours and at 7 days by measuring the diameter of the inhibition zone in millimeters (mm). Data were collected and analyzed using the repeated measure ANOVA and T-test. The level of significance was set at p<0.05.
Results: The antibacterial efficacy of the four studied dental cavity liners differed at different time intervals (p<0.001), but there were no statically significant differences in the antibacterial activity against the two bacteria types (p=0.342), or between the four types of dental cavity liners (p=0.07).
Conclusion: According to the results of the present research, the antibacterial activities of Ionobond, Ionoseal, Ionosit and Vitrebond dental cavity liners were not significantly different and decreased over time. |
topic |
Dental liners Antibacterial effect Streptococcus mutans Lactobacillus casei Fluoride-releasing dental materials |
url |
http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-567-1&slc_lang=en&sid=1 |
work_keys_str_mv |
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